Enhancing the Toughness of Regenerated Silk Fibroin Film through Uniaxial Extension

Yin, Jianwei; Chen, Er-Qiang; Porter, David; Shao, Zhengzhong

doi:10.1021/bm100643q

Cited by 81 publications

(84 citation statements)

References 39 publications

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“…27-29 In addition, after the membrane was stretched, its maximum absorbance shifted further toward 1630 cm −1 , and the birefringence also sharply increased (Fig. 2f), indicating the content of β-sheet molecular structures was significantly increased by stretching, agreeing with previous studies of natural silk materials 30-32 . As far as we can determine, the 1300% strain of our silk films is the highest strain value attained for silk materials (Fig.…”

Section: Resultssupporting

confidence: 90%

Printing of stretchable silk membranes for strain measurements

et al. 2016

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Quantifying the deformation of biological tissues under mechanical loading is crucial to understand its biomechanical response in physiological conditions and important for designing materials and treatments for biomedical applications. However, strain measurements for biological tissues subjected to large deformations and humid environments are challenging for conventional methods due to several limitations such as strain range, boundary conditions, surface bonding and biocompatibility. Here we propose the use of silk solutions and printing to synthesize prototype strain gauges for large strain measurements in biological tissues. The study shows that silk-based strain gauges can be stretched up to 1300% without failure, which is more than two orders of magnitude larger than conventional strain gauges, and the mechanics can be tuned by adjusting ion content. We demonstrate that the printing approach can accurately provide well bonded florescent features on the silk membranes using designs which can accurately measure strain in the membrane. The results show that these new strain gauges measure large deformations in the materials by eliminating the effects of sliding from the boundaries, making the measurements more accurate than direct outputs from tensile machines.

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Section: Resultssupporting

confidence: 90%

Printing of stretchable silk membranes for strain measurements

et al. 2016

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“…Furthermore, the values of 1.1±0.6 and 1.5±0.6 GPa obtained for the 6mer and 6mer+BSP films are close to the values obtained for silk fibroin films, 2.7 GPa 40 but still far from the values of 20–22 GPa for stiffness attributed to the major ampullate dragline silk from the spider Nephila clavipes 41 . This difference indicates that there remains a lot to learn about processing and assembly of silk proteins related to functional properties.…”

Section: Discussionsupporting

confidence: 79%

AFM Study of Morphology and Mechanical Properties of a Chimeric Spider Silk and Bone Sialoprotein Protein for Bone Regeneration

et al. 2011

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Atomic force microscopy (AFM) was used to assess a new chimeric protein consisting of a fusion protein of the consensus repeat for Nephila clavipes spider dragline protein and bone sialoprotein (6mer+BSP). The elastic modulus of this protein in film form was assessed through force curves, and film surface roughness was also determined. The results showed a significant difference between the elastic modulus of the chimeric silk protein, 6mer+BSP, and control films consisting of only the silk component (6mer). The behaviour of the 6mer+BSP and 6mer proteins in aqueous solution in the presence of calcium (Ca) ions was also assessed to determine interactions between the inorganic and organic components related to bone interactions, anchoring and biomaterial network formation. The results demonstrated the formation of protein networks in the presence of Ca2+ ions, characteristics that may be important in the context of controlling materials assembly and properties related to bone-formation with this new chimeric silk-BSP protein.

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“…However, the moduli of all three samples were similar. As SF possesses favorable mechanical properties, the improvements in mechanical properties following the grafting of SF were expected [19], while the modulus of the surface-modified samples was predominantly determined by the intrinsic chemistry of the bulk material, that is, PU.…”

Section: Resultsmentioning

confidence: 99%

Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering

Shen

Guo

et al. 2013

BioMed Research International

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In the field of tissue engineering, polymeric materials with high biocompatibility like polylactic acid and polyglycolic acid have been widely used for fabricating living constructs. For hypopharynx tissue engineering, skeletal muscle is one important functional part of the whole organ, which assembles the unidirectionally aligned myotubes. In this study, a polyurethane (PU) scaffold with microchannel patterns was used to provide aligning guidance for the seeded human myoblasts. Due to the low hydrophilicity of PU, the scaffold was grafted with silk fibroin (PU-SF) or gelatin (PU-Gel) to improve its cell adhesion properties. Scaffolds were observed to degrade slowly over time, and their mechanical properties and hydrophilicities were improved through the surface grafting. Also, the myoblasts seeded on PU-SF had the higher proliferative rate and better differentiation compared with those on the control or PU-Gel. Our results demonstrate that polyurethane scaffolds seeded with myoblasts hold promise to guide hypopharynx muscle regeneration.

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Enhancing the Toughness of Regenerated Silk Fibroin Film through Uniaxial Extension

Cited by 81 publications

References 39 publications

Printing of stretchable silk membranes for strain measurements

Printing of stretchable silk membranes for strain measurements

AFM Study of Morphology and Mechanical Properties of a Chimeric Spider Silk and Bone Sialoprotein Protein for Bone Regeneration

Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering

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