Enhancing The Antischistosomal Activity of Carvacryl Acetate Using Nanoemulsion

Abstract

Aim: To formulate a carvacryl acetate nanoemulsion (CANE) and test its antischistosomal activity. Materials & methods: CANE was prepared and tested in vitro on Schistosoma mansoni adult worms and both human and animal cell lines. Next, CANE was administered orally to mice infected with either a prepatent infection or a patent infection of S. mansoni. Results: CANE was stable during 90 days of analysis. CANE showed in vitro anthelmintic activity, and no cytotoxic effects were observed. In vivo, CANE was more effective than the free compounds in reducing worm burden and egg production. Treatment with CANE was more effective for prepatent infections than praziquantel. Conclusion: CANE improves antiparasitic properties and may be a promising delivery system for schistosomiasis treatment.

Antihistamines H1 as Potential Anthelmintic Agents against the Zoonotic Parasite Angiostrongylus cantonensis

“…4K-UHD monitor system (LG Electronics, Taubaté, SP, Brazil). 38 Larval motility, scored for an effect ≥60%, was categorized as follows: 1 (immotile), 2 (intermittent head or tail shaking), 3 (sluggish and motile), and 4 (highly active and motile). 14 Images were captured using a Motic AE2000 inverted microscope (Vancouver, Canada) equipped with a Motic ultrahigh definition (UHD) camera.…”

“…Larval viability was assessed immediately post drug addition (time 0) and after 2, 6, 12, and 24 h using an inverted microscope (BEL Engineering INV100, Monza, MB, Italy) equipped with a BEL Engineering ultrahigh definition (UHD) camera and a 48 in. 4K-UHD monitor system (LG Electronics, Taubaté, SP, Brazil) . Larval motility, scored for an effect ≥60%, was categorized as follows: 1 (immotile), 2 (intermittent head or tail shaking), 3 (sluggish and motile), and 4 (highly active and motile) .…”

Nanoemulsion Improves the Anti-Inflammatory Effect of Intraperitoneal and Oral Administration of Carvacryl Acetate

“…Previously, our group demonstrated that the nanoemulsification process improves the anthelmintic activity of CA via oral administration using a model of mice infected with Schistosoma mansoni [ 27 ]. In the present work, the influence of the mixture of surfactants on the stability of the CA nanoemulsion (CANE) was studied.…”