Enhancing magnetic resonance imaging tumor detection with fluorescence intensity and lifetime imaging

Erten, Ahmet; Hall, D.; Hoh, Carl K.; Cao, Hop S. Tran; Kaushal, Sharmeela; Esener, Sadik C.; Hoffman, Robert M.; Bouvet, Michael; Chen, James; Kesari, Santosh; Makale, Milan

doi:10.1117/1.3509111

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…28, 29, 30, 31, 32, 33 Alteration of the microenvironment of the fluorescent moiety on target binding can result in a shortening or lengthening of the fluorescence lifetime. This difference can be used to distinguish between unbound and bound agent in the target tissue as it is independent of intensity variations or local concentrations of the fluorophore.…”

Section: Discussionmentioning

confidence: 99%

Optical imaging of cell death in traumatic brain injury using a heat shock protein-90 alkylator

et al. 2013

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Traumatic brain injury is a major public health concern and is characterised by both apoptotic and necrotic cell death in the lesion. Anatomical imaging is usually used to assess traumatic brain injuries and there is a need for imaging modalities that provide complementary cellular information. We sought to non-invasively image cell death in a mouse model of traumatic brain injury using a near-infrared fluorescent conjugate of a synthetic heat shock protein-90 alkylator, 4-(N-(S-glutathionylacetyl) amino) phenylarsonous acid (GSAO). GSAO labels both apoptotic and necrotic cells coincident with loss of plasma membrane integrity. The optical GSAO specifically labelled apoptotic and necrotic cells in culture and did not accumulate in healthy organs or tissues in the living mouse body. The conjugate is a very effective imager of cell death in brain lesions. The optical GSAO was detected by fluorescence intensity and GSAO bound to dying/dead cells was detected from prolongation of the fluorescence lifetime. An optimal signal-to-background ratio was achieved as early as 3 h after injection of the probe and the signal intensity positively correlated with both lesion size and probe concentration. This optical GSAO offers a convenient and robust means to non-invasively image apoptotic and necrotic cell death in brain and other lesions.

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Section: Discussionmentioning

confidence: 99%

Optical imaging of cell death in traumatic brain injury using a heat shock protein-90 alkylator

et al. 2013

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“…Low dimensional semiconductor nanoparticles and/or quantum dots (QDs) have received great attention in cancer treatment nanotechnology. For instance, as-synthesized or modified nanoparticles are widely applied in tumor imaging, drug delivery 1,2 and diagnosis and treatment of cancer 3,4 . In this context, low dimensional nanomaterials based nano-formulated drugs are promising candidates to treat disease via targeted drug delivery 5,6 .…”

Section: Introductionmentioning

confidence: 99%

“…Low-dimensional semiconductor nanoparticles and/or quantum dots (QDs) have received great attention in cancer treatment nanotechnology. For instance, as-synthesized or modified nanoparticles are widely applied in tumor imaging, drug delivery, , and diagnosis and treatment of cancer. , In this context, low-dimensional nanomaterials-based nanoformulated drugs are promising candidates to treat disease via targeted drug delivery. , Targeted drug delivery-based biological treatment is highly precise and prevents side effects originating from systemic distribution of cytotoxic drugs and effectively controls cancer cell proliferation or tumor angiogenesis. , …”

Section: Introductionmentioning

confidence: 99%

Green-Synthesis-Derived CdS Quantum Dots Using Tea Leaf Extract: Antimicrobial, Bioimaging, and Therapeutic Applications in Lung Cancer Cells

Shivaji¹,

Suganya²,

Ponmurugan

et al. 2018

ACS Appl. Nano Mater.

132

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Low dimensional semiconductor quantum dots (<10 nm) have received great attention for potential use in biomedical applications (diagnosis and therapy) for which larger nanoparticles (>10 nm) are not suitable. Here, we demonstrate a green, biogenic synthesis route for making CdS quantum dots (QDs) with 2-5 nm particle size using tea leaf extract (Camellia sinensis) as a toxic-free particle stabilizing agent. We have explored the biological activity of these CdS QDs in different applications, namely; a) antibacterial activity b) bioimaging and c) apoptosis of lung cancer cells. The antibacterial activity of the CdS QDs has been studied against different types of bacteria growth, showing that CdS QDs effectively inhibit the bacterial growth and exhibit cytotoxicity towards A549 cancer cells when compared to a control (no QD treatment). We have compared this cytotoxicity effect on A549 cancer cells with a standard drug, cisplatin, showing comparable results. Additionally, these CdS QDs produce high contrast fluorescence images of A549 cancer cells indicating a strong interaction with the cancer cell. To further understand the role of CdS QDs in bioimaging and cytotoxicity effect in A549 cells, fluorescence emission and flow cytometry analysis were carried out. The fluorescence emission of CdS QDs were recorded with λexc= 410 nm, showing concentration dependence fluorescence emission centered at 670 nm. From the flow cytometry analysis, it is confirmed that the CdS QDs are arresting the A549 cell growth at the S phase of cell cycle, inhibiting further growth of lung cancer cell. The multifunctional advantages of Camellia sinensis extract mediated green CdS QDs will be of widespread interest in implementing in-vivo based bioimaging and therapeutic cancer treatment applications.

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“…Fluorescence lifetime holds promise in investigating the micro-environment of a lesion as demonstrated in fluorescence lifetime imaging microscopy (Cubeddu and et al 2002). For example, using a planar imaging system, Erten et al demonstrated that fluorescence intensity and lifetime images enhance the sensitivity of MRI in tumor detection (Erten et al 2010). However, they also mention that challenges remain since varying the tissue thickness modifies the apparent lifetime and fluorophore intensity.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

A photo-multiplier tube-based hybrid MRI and frequency domain fluorescence tomography system for small animal imaging

et al. 2011

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Fluorescence tomography (FT) is a promising molecular imaging technique that can spatially resolve both fluorophore concentration and lifetime parameters. However, recovered fluorophore parameters highly depend on the size and depth of the object due to the ill-posedness of the FT inverse problem. Structural a priori information from another high spatial resolution imaging modality has been demonstrated to significantly improve FT reconstruction accuracy. In this study, we have constructed a combined magnetic resonance imaging (MRI) and FT system for small animal imaging. A photo-multiplier tube (PMT) is used as the detector to acquire frequency domain FT measurements. This is the first MR-compatible time-resolved FT system that can reconstruct both fluorescence concentration and lifetime maps simultaneously. The performance of the hybrid system is evaluated with phantom studies. Two different fluorophores, Indocyanine Green (ICG) and 3-3′ Diethylthiatricarbocyanine Iodide (DTTCI), which have similar excitation and emission spectra but different lifetimes, are utilized. The fluorescence concentration and lifetime maps are both reconstructed with and without the structural a priori information obtained from MRI for comparison. We show that the hybrid system can accurately recover both fluorescence intensity and lifetime within 10% error for two 4.2 mm-diameter cylindrical objects embedded in a 38 mm-diameter cylindrical phantom when MRI structural a priori information is utilized.

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Enhancing magnetic resonance imaging tumor detection with fluorescence intensity and lifetime imaging

Cited by 4 publications

References 22 publications

Optical imaging of cell death in traumatic brain injury using a heat shock protein-90 alkylator

Optical imaging of cell death in traumatic brain injury using a heat shock protein-90 alkylator

Green-Synthesis-Derived CdS Quantum Dots Using Tea Leaf Extract: Antimicrobial, Bioimaging, and Therapeutic Applications in Lung Cancer Cells

A photo-multiplier tube-based hybrid MRI and frequency domain fluorescence tomography system for small animal imaging

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