Enhancing butanol production with Clostridium pasteurianum CH4 using sequential glucose–glycerol addition and simultaneous dual-substrate cultivation strategies

Kao, Wei Chen; Lin, De Shun; Cheng, Chung-Wei; Chen, Bor Yann; Lin, Ching-Nan; Chang, Jo Shu

doi:10.1016/j.biortech.2012.09.108

Cited by 45 publications

(31 citation statements)

References 23 publications

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“…(12). A high R 2 of greater than 0.9523 was obtained in this case with a random distribution of residual as shown in Fig.…”

Section: Resultsmentioning

confidence: 90%

“…Since it is a molecule rich in functionalities with three AOH groups, a great deal of research has been conducted to find new applications of glycerol for commercially viable, value-added products [2]. Currently, several processes have been developed to convert glycerol to other compounds such as through a catalytic reforming process [3][4][5], pyrolysis [6,7] or gasification [8,9] to produce hydrogen (H 2 ), biological process to produce ethanol and butanol [10][11][12], dehydration to produce acrolein [13,14], and hydrogenolysis to produce propanediol (PD) [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

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Route of glycerol conversion and product generation via TiO2-induced photocatalytic oxidation in the presence of H2O2

Jedsukontorn

Meeyoo

Saito

et al. 2015

Chemical Engineering Journal

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“…(12). A high R 2 of greater than 0.9523 was obtained in this case with a random distribution of residual as shown in Fig.…”

Section: Resultsmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Route of glycerol conversion and product generation via TiO2-induced photocatalytic oxidation in the presence of H2O2

Jedsukontorn

Meeyoo

Saito

et al. 2015

Chemical Engineering Journal

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“…The use of mixture of glucose and glycerol has also been investigated with C. pasteurianum to enhance butanol production [16, 17] and with C. beijerinckii NCIMB 8052 for in situ detoxification of furfural [27]. However, 1,3-PDO and acetone were still produced by C. pasteurianum and C. beijerinckii , respectively, grown on the mixture of glucose and glycerol.…”

Section: Resultsmentioning

confidence: 99%

“…Although C. pasteurianum is different from other butanol-producing clostridia in terms of utilizing glycerol as a sole carbon source for butanol production, it also produces 1,3-PDO as a major byproduct, instead of acetone. Attempts have been made to decrease the production of 1,3-PDO using a chemically mutated strain [15] and using a mixture of glucose and glycerol as substrates [16, 17]; but a certain amount of 1,3-propanediol was still produced.…”

Section: Introductionmentioning

confidence: 99%

Effective isopropanol–butanol (IB) fermentation with high butanol content using a newly isolated Clostridium sp. A1424

Youn

Lee

Kim

et al. 2016

Biotechnol Biofuels

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BackgroundAcetone–butanol–ethanol fermentation has been studied for butanol production. Alternatively, to achieve acetone-free butanol production, use of clostridium strains producing butanol and 1,3-propanediol (1,3-PDO) from glycerol, natural and engineered isopropanol–butanol–ethanol (IBE) producers has been attempted; however, residual 1,3-PDO and acetone, low IBE production by natural IBE producers, and complicated gene modification are limitations.ResultsHere, we report an effective isopropanol and butanol (IB) fermentation using a newly isolated Clostridium sp. A1424 capable of producing IB from various substrates with a small residual acetone. Notably, this strain also utilized glycerol and produced butanol and 1,3-PDO. After 46.35 g/L of glucose consumption at pH 5.5-controlled batch fermentation, Clostridium sp. A1424 produced 9.43 g/L of butanol and 13.92 g/L of IB at the productivity of 0.29 and 0.44 g/L/h, respectively, which are the highest values in glucose-based batch fermentations using natural IB producers. More interestingly, using glucose–glycerol mixtures at ratios ranging from 20:2 to 14:8 led to not only acetone-free and 1,3-PDO-free IB fermentation but also enhanced IB production along with a much higher butanol content (butanol/isopropanol ratio of 1.81 with glucose vs. 2.07–6.14 with glucose–glycerol mixture). Furthermore, when the mixture of glucose and crude glycerol at the ratio of 14:8 (total concentration of 35.68 g/L) was used, high butanol/isopropanol ratio (3.44) and butanol titer (9.86 g/L) were achieved with 1.4-fold enhanced butanol yield (0.28 g/g) and productivity (0.41 g/L/h) compared to those with glucose only at pH 5.5.ConclusionsA newly isolated Clostridium sp. A1424 was able to produce butanol and isopropanol from various carbon sources. The productivity and titer of butanol and total alcohol obtained in this study were higher than the previously reported results obtained using other natural IB producers. Use of the mixture of glucose and glycerol was successful to achieve acetone-free, 1,3-PDO-free, and enhanced IB production with higher yield, productivity, and selectivity of butanol compared to those with glucose only, providing great advantages from the perspective of carbon recovery to alcohols. This notable result could be accomplished by isolating an effective IB producer Clostridium sp. A1424 as well as by utilizing glucose–glycerol mixtures.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0650-7) contains supplementary material, which is available to authorized users.

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“…A második csoportnál is egy Clostridium tőnt ki az azonosítás során, azonban itt már találtam konkrét egyezést, a Clostridium pasteurianum faj mindkét vizsgált klón esetében. Ezt a fajt leginkább oldószergyártáshoz, valamint hidrogéntermelı fermentációknál alkalmazzák elıszeretettel [71,72], ezért is érdekes a jelenléte ebben a környezetben, fıként a megemelkedett hidrogén koncentrációt figyelembe véve.…”

Section: Otu-11 (302 Bp) éS Otu-12 (435 Bp)unclassified

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Enhancing butanol production with Clostridium pasteurianum CH4 using sequential glucose–glycerol addition and simultaneous dual-substrate cultivation strategies

Cited by 45 publications

References 23 publications

Route of glycerol conversion and product generation via TiO2-induced photocatalytic oxidation in the presence of H2O2

Route of glycerol conversion and product generation via TiO2-induced photocatalytic oxidation in the presence of H2O2

Effective isopropanol–butanol (IB) fermentation with high butanol content using a newly isolated Clostridium sp. A1424

Biogáztermelő mikrobaközösségek molekuláris biológiai vizsgálata

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