Enhancement of α-tocopherol content through transgenic and cell suspension culture systems in tobacco

Enhanced accumulation of phytosterols and phenolic compounds in cyclodextrin-elicited cell suspension culture of Daucus carota

“…5). However, these levels of α-tocopherol found were higher than those found in other SCC, e.g., sunflower [52], safflower [53] and tobacco [54]. When Geipel et al [52] characterized the production of α-tocopherol using photomixotrophic and heterorotrophic sunflower SCC, they found that the highest intracellular α-tocopherol levels (77 µg gDW -1 ) were obtained with a photomixotrophic cell culture grown in liquid MS supplemented with 0,5 mg L -1 naphthalene acetic acid, 0,5 mg L -1 benzylaminopurine, 15 g L -1 sucrose and maintained at 26 ºC and a light intensity of 125 µmol m -2 s -1 .…”

“…When Geipel et al [52] characterized the production of α-tocopherol using photomixotrophic and heterorotrophic sunflower SCC, they found that the highest intracellular α-tocopherol levels (77 µg gDW -1 ) were obtained with a photomixotrophic cell culture grown in liquid MS supplemented with 0,5 mg L -1 naphthalene acetic acid, 0,5 mg L -1 benzylaminopurine, 15 g L -1 sucrose and maintained at 26 ºC and a light intensity of 125 µmol m -2 s -1 . Likewise, sunflower and tobacco SCC produced 24 µg gFW -1 and 41 µg gFW -1 of α-tocopherol when 150 µM homogentisic acid alone or in combination with 200 µM phytol was added, respectively [55,54]. Moreover, Caretto et al [55] analyzed the levels of α-tocopherol obtained from different sunflower sources and observed a production level of 11,4 µg gFW -1 (100 µg gDW -1 ), 7,3 µg gFW -1 (70 µg gDW -1 ), 18,1 µg gFW -1 (180 µg gDW -1 ), and 24 µg gFW -1 (239 µg gDW -1 ) in tissues of hypocotyls, stems, leaves and SCC, respectively.…”

New strategies for the use of Linum usitatissimum cell factories for the production of bioactive compounds

“…In fact, Martinchik et al (2011) analyzed the nutritional facts of flaxseed and detected that the production of tocopherols was of 92 mg g seed À1 . When comparing these data with our tocopherol values, they are higher those found in flaxseeds and other SCC like sunflower (Geipel et al, 2014), safflower (Furuya et al, 1987) and tobacco (Harish et al, 2013). In fact, Geipel et al (2014) showed that the highest tocopherol levels (77 mg g DW À1 )…”

In vitro propagation, carotenoid, fatty acid and tocopherol content of Ajuga multiflora Bunge

“…α-Tocopherol is reported to have greater vitamin E activity and is largely present in leaf tissues of various plant species (Carvalho et al 2013 ). The content of α-tocopherol in A. multiflora leaves (101.1 μg g −1 FW) was higher than the value reported in Amaranthus caudatus (11.3 μg g −1 FW), Arabidopsis (10 μg g −1 FW), Chenopodium quinoa (1.98 μg g −1 FW), sunflower (14 μg g −1 FW) and tobacco (43 μg g −1 FW) cell cultures (Gala et al 2005 ; Antognoni et al 2008 ; Harish et al 2013 ) while lower than the value reported in Carthamus tinctorius (167.7 μg g −1 FW) and Vitis vinifera (261.5 μg g −1 FW) cell cultures (Chavan et al 2011 ; Cetin 2014 ).

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