Enhancement of the immune response in rabbits to a malaria DNA vaccine by immunization with a needle-free jet device

Aguiar, Joao C.; Hedstrom, Richard C.; Rogers, William O.; Charoenvit, Yupin; Sacci, John B.; Lanar, David E.; Majam, Victoria; Stout, Richard; Hoffman, Stephen L.

doi:10.1016/s0264-410x(01)00273-0

Cited by 86 publications

(46 citation statements)

References 20 publications

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“…route alone. 5 In humans, because of the limitations of administering more than 100 ml by i.d. at a single site by needle, it will be difficult to administer large quantities of DNA by this route alone.…”

Section: Route Of Injections and Dose Intervalmentioning

confidence: 99%

“…1 Because of their ease and simplicity of production, stability, and potential ease of combination, DNA vaccines have seemed to be an ideal method for establishing such immune responses. Our lab has systematically studied DNA malaria vaccines, either as single plasmids or as combinations in mice, 2-4 rabbits, 5 non-human primates, [6][7][8][9] and humans. [10][11][12] Additionally, we have also studied multiple methods to enhance the immunogenicity and protective efficacy of DNA vaccines in murine and non-human primate systems.…”

Section: Introductionmentioning

confidence: 99%

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Effect on antibody and T-cell responses of mixing five GMP-produced DNA plasmids and administration with plasmid expressing GM-CSF

et al. 2004

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One potential benefit of DNA vaccines is the capacity to elicit antibody and T-cell responses against multiple antigens at the same time by mixing plasmids expressing different proteins. A possible negative effect of such mixing is interference among plasmids regarding immunogenicity. In preparation for a clinical trial, we assessed the immunogenicity of GMP-produced plasmids encoding five Plasmodium falciparum proteins, PfCSP, PfSSP2, PfEXP1, PfLSA1, and PfLSA3, given as a mixture, or alone. The mixture induced higher levels of antibodies against whole parasites than did the individual plasmids, but was associated with a decrease in antibodies to individual P. falciparum proteins. T-cell responses were in general decreased by administration of the mixture. Immune responses to individual plasmids and mixtures were generally higher in inbred mice than in outbreds. In inbred BALB/c and C57BL/6 mice, coadministration of a plasmid expressing murine granulocyte-macrophage colony-stimulating factor (mGM-CSF), increased antibody and T-cell responses, but in outbred CD-1 mice, coadministration of mGM-CSF was associated with a decrease in antibody responses. Such variability in data from studies in different strains of mice underscores the importance of genetic background on immune response and carefully considering the goals of any preclinical studies of vaccine mixtures planned for human trials.

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Section: Route Of Injections and Dose Intervalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect on antibody and T-cell responses of mixing five GMP-produced DNA plasmids and administration with plasmid expressing GM-CSF

et al. 2004

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“…In addition to these advantages, microemulsions are easy to administer and offer several benefits for oral administration. Namely, microemulsions are ideal for the oral and nasal delivery of drugs and vaccines 25,26,27 . Microemulsion techniques are capable of delivering inorganic and organic nano-sized particles with minimal agglomeration because the reaction occurs in nano-sized domains.…”

Section: Microemulsionmentioning

confidence: 99%

“…Intestinal mucosa is constantly challenged with the considerable task of allowing the exchange of nutrients, ions and liquids across the intestinal epithelium in the presence of an enormous density of potentially harmful luminal antigens and microbes 27 .…”

Section: The Mucosal Immune Systemmentioning

confidence: 99%

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The Mucosal Immune System: Modulation by Microemulsion

França¹,

Honório-França²

2012

Microemulsions - An Introduction to Properties and Applications

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Nucleic Acid Vaccines

Buglione‐Corbett¹,

Suschak²,

Wang³

et al. 2013

Pharmaceutical Sciences Encyclopedia

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The use of safe and effective vaccines has become a fundamental aspect of global health.To this end, immunization with a nucleic‐acid‐based vaccine has led to new considerations in vaccination strategies.At least in preclinical studies, antigen‐encoding DNA plasmids are capable of eliciting a broad spectrum of immune responses against antigens from a variety of pathogens. As shown in this chapter, while DNA vaccination provides several advantages over other established vaccination strategies, further optimization is necessary before it becomes the predominant strategy in human patients.DNA vaccination has shown great promise in prime‐boost strategies and may provide a solution for pathogens that were previously thought unpreventable or untreatable.Their adaptability and ease of manipulation may prove to be the aspect that pushes them to the front line of vaccination.It appears that DNA vaccines are on the precipice of redefining the field of vaccinology.

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Enhancement of the immune response in rabbits to a malaria DNA vaccine by immunization with a needle-free jet device

Cited by 86 publications

References 20 publications

Effect on antibody and T-cell responses of mixing five GMP-produced DNA plasmids and administration with plasmid expressing GM-CSF

Effect on antibody and T-cell responses of mixing five GMP-produced DNA plasmids and administration with plasmid expressing GM-CSF

The Mucosal Immune System: Modulation by Microemulsion

Nucleic Acid Vaccines

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