Enhancement of stem cell differentiation to osteogenic lineage on hydroxyapatite-coated hybrid PLGA/gelatin nanofiber scaffolds

Sanaei‐rad, Parisa; Kashi, Tahereh Sadat Jafarzadeh; Seyedjafari, Ehsan; Soleimani, Masoud

doi:10.1016/j.biologicals.2016.09.002

Cited by 24 publications

(17 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…58 The reason for the superior cytocompatibility and osteoconductivity of HAP/mPLGA scaffold is probably due to presence of HAP favored by the oCS-Col I multilayer coating, which have been shown previously on other systems to promote attachment, spreading, proliferation, and osteogenic differentiation of stem cells. 21,59 Conversely, the surface modified with oCS-Col I multilayers might also have a supporting effect on cytocompatibility and BMSCs differentiation towards osteogenic lineage, as evidenced in our previous study. 35 Taking all these findings together, this study shows that the oCS-Col I multilayers on the surface of PLGA scaffolds, favored the deposition and the uniform distribution of nHAP coating, which enhances the mechanical and osteoconductivity properties.…”

Section: Discussionmentioning

confidence: 52%

“…OCN and OPN are important proteins involved in biomineralization, which are expressed later during osteogenesis . The reason for the superior cytocompatibility and osteoconductivity of HAP/mPLGA scaffold is probably due to presence of HAP favored by the oCS‐Col I multilayer coating, which have been shown previously on other systems to promote attachment, spreading, proliferation, and osteogenic differentiation of stem cells . Conversely, the surface modified with oCS‐Col I multilayers might also have a supporting effect on cytocompatibility and BMSCs differentiation towards osteogenic lineage, as evidenced in our previous study …”

Section: Discussionmentioning

confidence: 94%

“…For instance, blending of biopolymers such as collagen or gelatin with PLGA‐improved cell attachment and growth of the scaffolds . Furthermore, the mixing of inorganic nanoparticles like HAP with PLGA allows the production of composite scaffolds providing a microenvironment for better osteoblast attachment and differentiation, but also increased mechanical strength …”

Section: Introductionmentioning

confidence: 99%

“…18,19 Furthermore, the mixing of inorganic nanoparticles like HAP with PLGA allows the production of composite scaffolds providing a microenvironment for better osteoblast attachment and differentiation, but also increased mechanical strength. 20,21 Biomineralization is a powerful approach to produce biomimetic composites containing organic-inorganic materials for bone tissue engineering, as they can recapitulate the composition of bone, and thus support osteogenesis and improve the mechanical properties. 20,21 Several in vitro methods, including single-step, 22 bioinspired, 23 simulated body fluid (SBF), 24 and alternate-soaking mineralization 25 have been used to fabricate mineralized scaffolds.…”

mentioning

confidence: 99%

“…20,21 Biomineralization is a powerful approach to produce biomimetic composites containing organic-inorganic materials for bone tissue engineering, as they can recapitulate the composition of bone, and thus support osteogenesis and improve the mechanical properties. 20,21 Several in vitro methods, including single-step, 22 bioinspired, 23 simulated body fluid (SBF), 24 and alternate-soaking mineralization 25 have been used to fabricate mineralized scaffolds. However, the deposition of apatite minerals to polymeric materials at the micro and nanoscale remains a challenge.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Biomineralization improves mechanical and osteogenic properties of multilayer‐modified PLGA porous scaffolds

Kong

Wei

Groth

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Poly-(lactide-co-glycolide acid) (PLGA) has been widely investigated as scaffold material for bone tissue engineering owing to its biosafety, biodegradability, and biocompatibility. However, the bioinert surface of PLGA may fail in regulating cellular behavior and directing osteointegration between the scaffold and the host tissue. In this article, oxidized chondroitin sulfate (oCS) and type I collagen (Col I) were assembled onto PLGA surface via layer by layer technique (LbL) as an adhesive coating for the attachment of inorganic minerals. The multilayer-modified PLGA scaffold was mineralized in vitro to ensure the deposition of nanohydroxyapatite (nHAP). It was found that nHAP crystals were more uniformly and firmly attached on the multilayer-modified PLGA as compared with the pure PLGA scaffold, which remarkably improved PLGA surface and mechanical properties. Additionally, in vitro biocompatibility of PLGA scaffold, in terms of bone mesenchymal stem cells (BMSCs) attachment, spreading and proliferation was greatly enhanced by nHAP coating and multilayer deposition. Furthermore, the fabricated composite scaffold also shows the ability to promote the osteogenic differentiation of BMSCs through the up-regulation of osteogenic marker genes. Thus, this novel biomimetic composite scaffold might achieve a desirable therapeutic result for bone tissue regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2714-2725, 2018.

show abstract