Enhancement of osteoblast adhesion to UV-photofunctionalized titanium via an electrostatic mechanism

Iwasa, Fuminori; Hori, Norio; Ueno, Takeshi; Minamikawa, Hajime; Yamada, Masahiro; Ogawa, Takahiro

doi:10.1016/j.biomaterials.2009.12.024

Cited by 171 publications

(200 citation statements)

References 31 publications

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“…Vinculin expression was not only higher, but also emerged earlier and was concentrated more at the tip of the cellular projections on UV-treated surfaces than on untreated surfaces. In addition, osteoblasts on UV-treated surfaces showed an increase in the area within an individual cell at both the beginning and the end of the cell adhesion period, but no significant increase in density-to-cell area at the end of the cell adhesion period, a result similar to that obtained in our earlier study [16]. This indicates that UV-treatment of titanium surfaces does not directly up-regulate vinculin expression in osteoblasts, but rather results in the formation of focal adhesion patches as cell spread progresses.…”

Section: Discussionsupporting

confidence: 90%

“…As the confocal microscopic images in this Acta Biomater study show (Fig. 4), cell adhesion was promoted and enhanced on mirror-polished titanium surfaces after UV-treatment in a similar manner to that seen on roughened surfaces in our previous report [16].…”

Section: Discussionsupporting

confidence: 86%

“…Osteoblasts must adhere to titanium surfaces with various topographies depending on the device's structural and surface design. Taken together with the results of earlier studies on rough-surface titanium such as machined, acid-etched and sandblasted titanium [12][13][14][15][16], the present results obtained using very smooth, mirror-polished titanium surfaces indicate that UV-photofunctionalization technology can be universally applied to the bio-activation of titanium bulk material/devices, regardless of surface topography.…”

Section: Discussionsupporting

confidence: 82%

“…This phenomenon may be associated with the enhanced osteoblastic cell affinity observed on UV-treated titanium surfaces. In addition, a direct interaction between the cell and the UV-treated titanium surface was proposed in our previous experiment using a serum-free culture [16]. As described above, UV-treatment is known to change the physicochemical properties of the titanium surface, resulting in super-hydrophilicity 20 Acta Biomater and a reduced atomic percentage of carbon.…”

Section: Discussionmentioning

confidence: 94%

“…In fact, UV-enhanced cell adhesion was abrogated when UV-treated titanium surfaces were electrostatically neutralized by either removing the electric charge or masking with monovalent anions, while the surfaces maintained super-hydrophilicity [16]. Clarification of how interfacial signal transmission from material to cell results in osteoblastic affinity and adhesion strengthening on UV-treated titanium surfaces will be of great interest.…”

Section: Discussionmentioning

confidence: 99%

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Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

et al. 2010

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Ultraviolet (UV)-photofunctionalization of titanium substantially enhances the strength and quality of osseointegration by promoting osteogenic cellular attachment and proliferation. However, the mechanism underlying the initial interaction between the cells and the surface of the material remains to be elucidated, especially where the influence of surface roughness is excluded as a factor.We evaluated the effect of UV-photofunctionalization on the adhesive strength and cellular stiffness of a single osteoblast and its association with extent of cell spread, cytoskeletal development and focal adhesion assembly on a very smooth titanium surface. Rat bone marrow-derived osteoblasts were cultured on UV-treated or -untreated mirror-polished titanium disks. The mean critical shear force required to initiate detachment of a single osteoblast (n = 10) was over 2000 nN on a UV-treated surface at 3 hr incubation, which was 17 times greater than that on an untreated surface.The mean total energy required to complete the detachment of osteoblasts (n = 10) was consistently over 60 pJ on a UV-treated titanium surface after 24 hr culture, which was up to 42 times greater than that on an untreated surface. Cellular shear modulus, which represents cellular stiffness, was consistently greater on a UV-treated surface than on an untreated surface after 24 hr incubation (n = 10). This strengthening of cell adhesion and cellular mechanical properties on UV-treated titanium was accompanied by enhanced cell spread and actin fiber development and increased levels of vinculin expression. These results indicate that UV-photofunctionalization substantially strengthens osteoblast retention on titanium bulk material with no topographical features and that this is associated with enhancement of intracellular structural development during the cell adhesion process.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

et al. 2010

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Surface Chemistry of Vitamin: Pyridoxal 5′‐Phosphate (Vitamin B₆) as a Multifunctional Compound for Surface Functionalization

Lee

Kim

Lee

et al. 2015

Adv Funct Materials

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Vitamins are non‐toxic compounds that perform a variety of biological functions and also available in a large quantity. Other than the usage as food supplements, few attempts have been made to use them as functional materials. In this study, we report that vitamin B6, pyridoxal 5′‐phosphate (PLP), is a multi‐functional molecule for oxide surface chemistry. PLP‐immobilized surfaces exhibit superhydrophilicity and even hemophilicity, enhancing proliferation, migration, and differentiation of mammalian cells. Unlike existing molecules used so far in surface modification, PLP has an intrinsic chemical reactivity toward biomacromolecules due to the presence of the aldehyde group. In fact, RGD peptide is covalently tethered onto PLP surfaces directly in one step without any chemical activation. Furthermore, PLP‐functionalized implant device showed rapid bone healing. As vitamin B6 is a FDA approved molecule for human usage, the surface chemistry of vitamin B6 potentially allows a fast route for surface functionalized medical devices into clinic.

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Positioning of integrin β1, caveolin‐1 and focal adhesion kinase on the adhered membrane of spreading cells

Jokhadar

Majhenc

Svetina

et al. 2013

Cell Biology International

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We have investigated the relationship between the spreading of anchorage-dependent cells and the surface-density distribution of plasma membrane adhesion proteins. The surface positioning and density of integrin β1, caveolin-1 (cav-1), the phosphorylated caveolin-1 (p-cav-1) and the focal adhesion kinase (FAK) located on the adhering cell membrane (ACM) of HUVEC cells was studied. Imaging with TIRF microscopy was used, which enabled us to observe a few-nanometers-thin section of the cell above the plasma membrane in combination with image-based analyses. Integrin β1 and cav-1 have spatial interdependence on the ACM. Cells treated with substances that act on cell spreading caused changes in the size of the ACM area, as well as a redistribution of several proteins under investigation. Changes to the ACM area correlated positively with those to the surface density of the cav-1. The high integrin β1 and the low cav-1 surface density, and vice versa, following the treatments show that the presence of one of them not only spatially excludes, but also reduces, the occurrence of the other protein on the ACM, which indicates a regulative mechanism between integrin β1 and cav-1.

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Enhancement of osteoblast adhesion to UV-photofunctionalized titanium via an electrostatic mechanism

Cited by 171 publications

References 31 publications

Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

Surface Chemistry of Vitamin: Pyridoxal 5′‐Phosphate (Vitamin B₆) as a Multifunctional Compound for Surface Functionalization

Positioning of integrin β1, caveolin‐1 and focal adhesion kinase on the adhered membrane of spreading cells

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Enhancement of osteoblast adhesion to UV-photofunctionalized titanium via an electrostatic mechanism

Cited by 171 publications

References 31 publications

Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

Surface Chemistry of Vitamin: Pyridoxal 5′‐Phosphate (Vitamin B6) as a Multifunctional Compound for Surface Functionalization

Positioning of integrin β1, caveolin‐1 and focal adhesion kinase on the adhered membrane of spreading cells

Contact Info

Product

Resources

About

Surface Chemistry of Vitamin: Pyridoxal 5′‐Phosphate (Vitamin B₆) as a Multifunctional Compound for Surface Functionalization