Enhancement of neurite outgrowth using nano-structured scaffolds coupled with laminin

Koh, Hian Chye; Tan, Yong Zen; Chan, Casey K.; Ramakrishna, Seeram

doi:10.1016/j.biomaterials.2008.05.014

Cited by 468 publications

(377 citation statements)

References 39 publications

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“…A variety of materials of natural and synthetic origin, have been previously shown to promote adhesion, proliferation, neurite extension, and neuronal differentiation of neural cells in vitro and in vivo [26][27][28]. Synthetic polymer-based biomaterial scaffolds have the added advantage of controlled chemistries and mechanical properties [29,30], while enabling display or release of neurotrophic factors [31,32]. Of the various scaffold configurations proposed to date [27,28,30], electrospun polymer substrates have exhibited excellent neurogenic properties, due to their high surface area and porosity, and fibrous ECM-like geometries [33,34].…”

Section: Introductionmentioning

confidence: 99%

Oriented, Multimeric Biointerfaces of the L1 Cell Adhesion Molecule: An Approach to Enhance Neuronal and Neural Stem Cell Functions on 2-D and 3-D Polymer Substrates

et al. 2012

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This article focuses on elucidating the key presentation features of neurotrophic ligands at polymer interfaces. Different biointerfacial configurations of the human neural cell adhesion molecule L1 were established on two-dimensional films and three-dimensional fibrous scaffolds of synthetic tyrosine-derived polycarbonate polymers and probed for surface concentrations, microscale organization, and effects on cultured primary neurons and neural stem cells. Underlying polymer substrates were modified with varying combinations of protein A and poly-d-lysine to modulate the immobilization and presentation of the Fc fusion fragment of the extracellular domain of L1 (L1-Fc). When presented as an oriented and multimeric configuration from protein A-pretreated polymers, L1-Fc significantly increased neurite outgrowth of rodent spinal cord neurons and cerebellar neurons as early as 24 h compared to the traditional presentation via adsorption onto surfaces treated with poly-d-lysine. Cultures of human neural progenitor cells screened on the L1-Fc/polymer biointerfaces showed significantly enhanced neuronal differentiation and neuritogenesis on all protein A oriented substrates. Notably, the highest degree of βIII-tubulin expression for cells in 3-D fibrous scaffolds were observed in protein A oriented substrates with PDL pretreatment, suggesting combined effects of cell attachment to polycationic charged substrates with subcellular topography along with L1-mediated adhesion mediating neuronal differentiation. Together, these findings highlight the promise of displays of multimeric neural adhesion ligands via biointerfacially engineered substrates to “cooperatively” enhance neuronal phenotypes on polymers of relevance to tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Oriented, Multimeric Biointerfaces of the L1 Cell Adhesion Molecule: An Approach to Enhance Neuronal and Neural Stem Cell Functions on 2-D and 3-D Polymer Substrates

et al. 2012

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“…(ii) physical absorption of biomolecules: a variety of ECM components such as gelatin, collagen, laminin and fibronectin have been physically immobilized at ES fibre surface, generally after pre-treatement with plasma [106,107].…”

Section: Surface Functionalizationmentioning

confidence: 99%

“…Scaffold morphology is controlled by the type of solvent, polymer concentration and phase separation temperature. This technique can be used to fabricate scaffolds from many types of polymers and polymeric composite materials, obtaining highly interconnected pores with tuneable pore size and also combined macro and microporous structures [107,108]. The method enables to produce also nanofibrous networks with porosity of 98% and fibre diameters in the range 50-500 nm [3].…”

Section: Thermally Induced Phase Separationmentioning

confidence: 99%

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

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and some lactide copolymers) and of non-commercial polyesters (i.e. poly-ω-pentadecalactone and some of its copolymers) were explored and discussed.Two techniques were employed to fabricate scaffolds: supercritical carbon dioxide (scCO 2 ) foaming and electrospinning (ES). The former is a powerful technology that enables to produce 3D microporous foams by avoiding the use of solvents that can be toxic to mammalian cells. The scCO 2 process, which is II commonly applied to amorphous polymers, was successfully modified to foam a highly crystalline poly(ω-pentadecalactone-co-ε-caprolactone) copolymer and the effect of process parameters on scaffold morphology and thermo-mechanical properties was investigated.In the course of the present research activity, sub-micrometric fibrous nonwoven meshes were produced using ES technology. Electrospun materials are considered highly promising scaffolds because they resemble the 3D organization of native extra cellular matrix. A careful control of process parameters allowed to fabricate defect-free fibres with diameters ranging from hundreds of nanometers to several microns, having either smooth or porous surface. Moreover, versatility of ES technology enabled to produce electrospun scaffolds from different polyesters as well as "composite" non-woven meshes by concomitantly electrospinning different fibres in terms of both fibre morphology and polymer material. The 3D-architecture of the electrospun scaffolds fabricated in this research was controlled in terms of mutual fibre orientation by properly modifying the instrumental apparatus. This aspect is particularly interesting since the micro/nano-architecture of the scaffold is known to affect cell behaviour.Since last generation scaffolds are expected to induce specific cell response, the present research activity also explored the possibility to produce electrospun scaffolds bioactive towards cells. Bio-functionalized substrates were obtained by loading polymer fibres with growth factors (i.e. biomolecules that elicit specific cell behaviour) and it was demonstrated that, despite the high voltages applied during electrospinning, the growth factor retains its biological activity once

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“…22 Biofunctionalization of ESFs made of biodegradable synthetic polymers is usually achieved by two strategies. First, polymers are co-electrospun with biomacromolecules such as ECM adhesive proteins to form composite electrospun scaffolds to enhance cell adhesion, for example, PCL/ collagen 25 and PLLA/laminin 26 composite ESFs. Nevertheless, it is sometimes difficult to find a suitable solvent to simultaneously dissolve hydrophobic synthetic polymer and hydrophilic proteins for electrospinning.…”

mentioning

confidence: 99%

Electrospun PLGA Fibers Incorporated with Functionalized Biomolecules for Cardiac Tissue Engineering

Lee

Lin

et al. 2014

Tissue Engineering Part A

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Structural similarity of electrospun fibers (ESFs) to the native extracellular matrix provides great potential for the application of biofunctional ESFs in tissue engineering. This study aimed to synthesize biofunctionalized poly (Llactide-co-glycolide) (PLGA) ESFs for investigating the potential for cardiac tissue engineering application. We developed a simple but novel strategy to incorporate adhesive peptides in PLGA ESFs. Two adhesive peptides derived from laminin, YIGSR, and RGD, were covalently conjugated to poly-L-lysine, and then mingled with PLGA solution for electrospinning. Peptides were uniformly distributed on the surface and in the interior of ESFs. PLGA ESFs incorporated with YIGSR or RGD or adsorbed with laminin significantly enhanced the adhesion of cardiomyocytes isolated from neonatal rats. Furthermore, the cells were found to adhere better on ESFs compared with flat substrates after 7 days of culture. Immunofluorescent staining of F-actin, vinculin, a-actinin, and Ncadherin indicated that cardiomyocytes adhered and formed striated a-actinin better on the laminin-coated ESFs and the YIGSR-incorporated ESFs compared with the RGD-incorporated ESFs. The expression of a-myosin heavy chain and b-tubulin on the YIGSR-incorporated ESFs was significantly higher compared with the expression level on PLGA and RGD-incorporated samples. Furthermore, the contraction of cardiomyocytes was faster and lasted longer on the laminin-coated ESFs and YIGSR-incorporated ESFs. The results suggest that aligned YIGSRincorporated PLGA ESFs is a better candidate for the formation of cardiac patches. This study demonstrated the potential of using peptide-incorporated ESFs as designable-scaffold platform for tissue engineering.

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Enhancement of neurite outgrowth using nano-structured scaffolds coupled with laminin

Cited by 468 publications

References 39 publications

Oriented, Multimeric Biointerfaces of the L1 Cell Adhesion Molecule: An Approach to Enhance Neuronal and Neural Stem Cell Functions on 2-D and 3-D Polymer Substrates

Oriented, Multimeric Biointerfaces of the L1 Cell Adhesion Molecule: An Approach to Enhance Neuronal and Neural Stem Cell Functions on 2-D and 3-D Polymer Substrates

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Electrospun PLGA Fibers Incorporated with Functionalized Biomolecules for Cardiac Tissue Engineering

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