Enhancement of Liver Regeneration by the Association of Hyptis pectinata with Laser Therapy

Melo, Gustavo Barreto; Silva, Renata L.; Melo, Valdinaldo Aragão de; Lima, Sônia Oliveira; Antoniolli, Ângelo Roberto; Castro-E-Silva, Tiago; Marcassa, Luís Gustavo; Bagnato, Vanderlei Salvador; Zucoloto, Sérgio; Ramalho, Leandra Naíra Zambelli; Ramalho, Fernando Silva; Castro‐e‐Silva, Orlando

doi:10.1007/s10620-005-2670-1

Cited by 13 publications

(18 citation statements)

References 23 publications

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“…The Hyptis ovalifolia has presented antimicrobial effect 5 . Also, the Hyptis pectinata has enhanced liver regeneration [6][7][8] . However no article about the effect of Hyptis fructicosa on liver regeneration was found.…”

Section: Introductionmentioning

confidence: 99%

Proliferative effect of aqueous extract of Hyptis fructicosa on liver regeneration after partial hepatectomy in rats

Lima

Viana²,

Santana³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To evaluate the effect of aqueous extract of Hyptis fructicosa on hepatic regeneration after partial hepatectomy in rats. METHODS:Sixteen rats were divided in two groups: C (Control Group) and HF (Whose rats received aqueous extract of Hyptis fructicosa during 4 days using the dose of 100 mg/kg/day). On the consecutive day of this treatment, the animals of both groups underwent hepatectomy of about 67% of liver. Twenty four hours later, they were sacrificed, and the remaining mass of liver was removed and prepared to be studied through the PCNA immunohistochemical technique. RESULTS:The liver regeneration index of HF group was 53.56 ± 18.91%, while in C group was 21.12 ± 8.29% (p=0.0003). CONCLUSION:These results show that the administration of aqueous extract of Hyptis fructicosa using the dose of 100mg/kg/day increased the hepatocyte proliferation in the group HF.Key words: Plants, Medicinal. Hepatectomy. Proliferating Cell Nuclear Antigen. Liver Regeneration. Hyptis. Rats. RESUMO OBJETIVO:Avaliar o efeito do extrato aquoso da Hyptis fructicosa sobre a regeneração hepática após hepatectomia parcial em ratos. MÉTODOS:Dezesseis ratos foram divididos em dois grupos: C (grupo controle) e HF (ratos que receberam o extrato aquoso da Hyptis fructicosa durante quatro dias na dose de 100mg/kg/dia). No dia consecutivo deste tratamento, os animais de ambos os grupos foram submetidos a hepatectomia de aproximadamente 67% do fígado. Vinte e quatro horas depois, eles foram sacrificados, e que a massa restante do fígado foi retirado e preparado para ser estudado através da técnica de imuno-histoquímica PCNA. RESULTADOS:O índice de regeneração hepática do grupo HF foi 53,56 ± 18,91%, enquanto no grupo C foi de 21,12 ± 8,29%(p=0,0003). CONCLUSÃO:Estes resultados mostram que a administração do extrato aquoso da Hyptis fructicosa na dose de 100mg/kg/dia aumentou a proliferação de hepatócitos no grupo HF.

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Section: Introductionmentioning

confidence: 99%

Proliferative effect of aqueous extract of Hyptis fructicosa on liver regeneration after partial hepatectomy in rats

Lima

Viana²,

Santana³

et al. 2012

Acta Cir. Bras.

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“…As in this study, leaves-derived extracts of Peumus boldus induced hepatocytes proliferation; it is possible to suggest that this natural product display trophic effects on liver tissue. The precise mechanism underlying the proliferative effect displayed by AEPB is still unclear, but it is possible to suppose a possible relation with the well-established antioxidant properties of the bioactive compounds present in the extract, such as alkaloids and flavonoids 8,9,13,29,30 . However, although we provide evidence of a possible hepatotrophic effect of AEPB, further investigations are still required in order to clarify the biochemical mechanism responsible for such biological activity.…”

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confidence: 99%

The effect of the aqueous extract Peumus boldus on the proliferation of hepatocytes and liver function in rats submitted to expanded hepatectomy

Figueiredo

Santana²,

Nardelli

et al. 2016

Acta Cir. Bras.

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PURPOSE:To assess the effect of aqueous extract of Peumus Boldus (AEPB) on the liver proliferative response after parcial hepatectomy of 70% (PH) in rodents. METHODS:Twenty Wistar rats were divided in two groups: AEPB100 (whose rats received 100mg/Kg of AEPB, once a day, orally, in 4 days prior to the first surgical procedure) and Vehicle (whose rats were treated similarly with distilled water). Both groups underwent PH. After 24 hours the remaining livers were removed for studying the proliferation of hepatocytes by Ki-67 and 2mL of blood were collected for serological assessment: cholesterol, glucose, triglycerides, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and total, direct and indirect bilirubin. All data were analyzed by Gaussian distribution. Statistically significant differences between mean values were analyzed using T Student's test. Non-Gaussian data were analyzed using Mann-Whitney's test. RESULTS:The liver of all these rats presented positive staining of Ki-67, indicating liver proliferation. Laboratory results showed no significant difference in serum values between the analyzed groups. The analysis of Ki-67 was significantly more positive in AEPB100 group than in Vehicle group. CONCLUSION:Aqueous extract of Peumus Boldus acute administration exerts significant positive effect on liver regeneration after 24h in rats that underwent parcial hepatectomy, while maintaining unchanged hepatic function.

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“…The extract showed positive results on the abdominal contortions and hot plate analgesy models and antinfl ammatory activity in the paw oedema model (Bispo et al, 2001;Arrigoni-Blank et al, 2005;Falcão et al, 2005). The same laboratory demonstrated that the aqueous extract of H. pectinata accelerated liver regeneration after hepatectomy in rats (Melo et al, 2005). Another species of Hyptis, for instance, H. martiusii, common in the northeast of Brazil, showed activity against larvae of Aedes aegypti (Costa et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Effects of the aqueous extract from Hyptis pectinata leaves on rodent central nervous system

Bueno¹,

Moreira²,

Silva³

et al. 2006

Rev. bras. farmacogn.

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RESUMO: "Efeitos do extrato aquoso das folhas da Hyptis pectinata sobre o sistema nervoso central de roedores". Neste trabalho foram estudados os efeitos do extrato aquoso (EA) obtido das folhas da Hyptis pectinata sobre o sistema nervoso central de roedores. Os seguintes modelos experimentais foram utilizados em camundongos, machos e fêmeas, da linhagem Wistar: "screening" farmacológico, campo aberto, nado forçado, hipotermia induzida por apomorfi na, labirinto em cruz elevado e tempo de sono induzido por tiopental. O extrato foi administrado por via oral em dose única, 30 minutos antes de cada teste. No "screening" farmacológico o EA (400 mg/kg, p.o., n = 3) diminuiu a freqüência do comportamento de auto-limpeza. No teste do campo aberto o EA diminuiu o tempo gasto em auto-limpeza (AE400: 51,3 ± 8,8, q = 5,513, P < 0.01, n = 10) de maneira similar ao diazepam (control: 107,3 ± 14,2; diazepam: 15,4 ± 4,3, q = 9,049, P < 0.001, n = 10). No teste do nado forçado o EA (400 mg/kg) diminuiu o tempo de imobilidade (con: 181,3 ± 7,2 s; imip: 91,6 ± 8,9 s, q = 7,958, P < 0,001; AE400: 111,6 ± 14,5 s, q = 6,193, P < 0,001, n = 9) indicando um possível efeito antidepressivo. Isto foi confi rmado no modelo de hipotermia induzida pela apomorfi na onde o EA (200 mg/kg) antagonizou o efeito da apomorfi na (AE200: -0,27 o C, q = 5,588, P < 0,001, n = 10) de maneira similar à imipramina (5 mg/kg) (control: -1,08 o C; imipramine: 0,02 o C, q = 7,589, P < 0,001, n = 10). Nenhum efeito do EA foi observado nos testes do labirinto em cruz elevado e do tempo de sono induzido por tiopental. Nossos resultados sugerem um possível efeito antidepressivo do extrato aquoso obtido das folhas da Hyptis pectinata. Unitermos:Hyptis pectinata, Lamiaceae, plantas medicinais, depressão. ABSTRACT:The effects of the aqueous extract (AE) from Hyptis pectinata leaves was studied on rodent central nervous system (CNS). Pharmacological screening, open fi eld, forced swimming, apomorphine-induced hypothermia, elevated plus maze and thiopental-induced sleep tests were used in male and female Swiss mice and Wistar rats. The AE was admnistered orally in single doses 30 minutes before each test. In the screening test the AE (400 mg/kg, p.o., n =3) decreased the frequency of grooming behavior. In the open fi eld test it decreased the amount of time rats spent grooming (AE400: 51.3 ± 8.8, q = 5.513, P < 0.01, n = 10), similarly to diazepam (control: 107.3 ± 14.2; diazepam: 15.4 ± 4.3, q = 9.049, P < 0.001, n = 10). In the forced swimming test the AE (400 mg/kg) decreased the immobility time (con: 181.3 ± 7.2 s; imip: 91.6 ± 8.9 s, q = 7.958, P < 0.001; AE400: 111.6 ± 14.5 s, q = 6.193, P < 0.001, n = 9), indicating a possible antidepressive effect. This was confi rmed in the apomorphine-induced hypothermia model where the extract (200 mg/kg) antagonized the apomorphine effect (AE200: -0.27 o C, q = 5.588, P < 0.001, n = 10), similarly to imipramine (imipramine: 5 mg/kg) (control: -1.08 o C; imipramine: 0.02 o C, q = 7.589, P < 0.001, n = 10). No effect of the...

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Enhancement of Liver Regeneration by the Association of Hyptis pectinata with Laser Therapy

Cited by 13 publications

References 23 publications

Proliferative effect of aqueous extract of Hyptis fructicosa on liver regeneration after partial hepatectomy in rats

Proliferative effect of aqueous extract of Hyptis fructicosa on liver regeneration after partial hepatectomy in rats

The effect of the aqueous extract Peumus boldus on the proliferation of hepatocytes and liver function in rats submitted to expanded hepatectomy

Effects of the aqueous extract from Hyptis pectinata leaves on rodent central nervous system

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