Enhancement of lipid production using biochemical, genetic and transcription factor engineering approaches

Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum

“…In addition to the important roles of diatoms in aquatic ecosystems and the global cycling of carbon, under certain circumstances, some diatoms can store carbon and energy in the form of lipids (predominantly triacylglycerols [TAGs]), suggesting the possibility of cultivating diatoms for biodiesel production (Courchesne et al, 2009). Biodiesel produced from microalgae shows considerable promise as a potential major contributor to the displacement of petroleum-based fuels and thus has captured the interest of researchers and entrepreneurs around the world (Chisti, 2007).…”

“…Biodiesel produced from microalgae shows considerable promise as a potential major contributor to the displacement of petroleum-based fuels and thus has captured the interest of researchers and entrepreneurs around the world (Chisti, 2007). In recent years, major efforts have been made to increase microalgal lipid production using biochemical and genetic engineering approaches (Courchesne et al, 2009;Aguirre et al, 2013). While these approaches have had some success, further investigation is required to understand the biochemical and molecular mechanisms underlying lipid production in microalgae.…”

Lipid production by Lipomyces starkeyi cells in glucose solution without auxiliary nutrients

“…One of the prominent renewable fuels is biodiesel, which can be produced with the raw materials such as vegetable oil, animal fats and potentially, microbial lipid (Li et al, 2008;Meng et al, 2009;Courchesne et al, 2009;Gao et al, 2009). …”

Metabolite Profiling and Integrative Modeling Reveal Metabolic Constraints for Carbon Partitioning under Nitrogen Starvation in the Green Algae Haematococcus pluvialis

“…Similar results have been obtained in studies of species of the family Chlorellaceae; for instance, under sulfur deficiency, lipid accumulation begins with a reduction in stored starch (13), and in Chlorella UTEX29, the number of lipid bodies increased while starch granules decreased after 24 h of nitrogen deprivation (14). * This work was financially supported by the European Commission's Sev-Several studies have investigated the interplay of FA and CH biosynthesis and its regulation in microalgae, often with the aim of enhancing oil accumulation in the cell (15)(16)(17)(18)(19)(20). For example, it was shown that inactivation of ADP-glucose pyrophosphorylase in a Chlamydomonas starchless mutant can lead to a 10-fold increase in triacylglycerol (TAG) (21); however, these findings are still being debated (3).…”