Enhancement of lipid production and fatty acid profiling in Chlamydomonas reinhardtii, CC1010 for biodiesel production

Karpagam, Rathinasamy; Preeti, Ranjan; Ashokkumar, Balasubramaniem; Varalakshmi, Perumal

doi:10.1016/j.ecoenv.2015.03.015

Cited by 66 publications

(22 citation statements)

References 34 publications

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“…This species was also applied to convert oxygen-pretreated Kraft lignin into valuable lipids [107]. Chlorella protothecoides Glucose [114] Tetraselmis elliptica Photoautotrophic (Flory medium) [115] C. vulgaris NIES-227 Heterotrophic cultivation on glucose under nitrogen limitation [116] Auxenochlorella protothecoides Organosolv pretreated birch biomass hydrolysates [71] Auxenochlorella protothecoides Organosolv pretreated spruce biomass hydrolysates [71] Botryococcus braunii Photoautotrophic (modified Chu 13 medium) [117] Chlamydomonas reinhardtii, CC1010 Photoheterotrophic (TAPN -+ 0.1% glucose) [118] Yeast and filamentous fungi Cryptococcus sp. (KCTC 27583) Pretreated banana peel [119] Rhodosporidium kratochvilovae HIMPA1…”

Section: Oleaginous Bacteriamentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

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Microorganisms are known to be natural oil producers in their cellular compartments. Microorganisms that accumulate more than 20% w/w of lipids on a cell dry weight basis are considered as oleaginous microorganisms. These are capable of synthesizing vast majority of fatty acids from short hydrocarbonated chain (C6) to long hydrocarbonated chain (C36), which may be saturated (SFA), monounsaturated (MUFA), or polyunsaturated fatty acids (PUFA), depending on the presence and number of double bonds in hydrocarbonated chains. Depending on the fatty acid profile, the oils obtained from oleaginous microorganisms are utilized as feedstock for either biodiesel production or as nutraceuticals. Mainly microalgae, bacteria, and yeasts are involved in the production of biodiesel, whereas thraustochytrids, fungi, and some of the microalgae are well known to be producers of very long-chain PUFA (omega-3 fatty acids). In this review article, the type of oleaginous microorganisms and their expertise in the field of biodiesel or omega-3 fatty acids, advances in metabolic engineering tools for enhanced lipid accumulation, upstream and downstream processing of lipids, including purification of biodiesel and concentration of omega-3 fatty acids are reviewed.

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Section: Oleaginous Bacteriamentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

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“…Earlier reports suggested that C.reinhardtii cells lack glucose uptake mechanism and that they cannot grow heterotrophically (dark) in the presence of glucose as the sole carbon source (Doebbe et al , 2007; Karpagam et al , 2015; Sager et al , 1953). However, the uptake of glucose in C.reinhardtii cells in light versus dark has not been carefully studied so far.…”

Section: Resultsmentioning

confidence: 99%

“…However, the uptake of glucose in C.reinhardtii cells in light versus dark has not been carefully studied so far. It is also important to note here that some Chlamydomonas species are known to utilize glucose efficiently to support their growth both hetero- and mixotrophically (Bennett et al , 1972; Karpagam et al , 2015). In order to rationalize glucose addition effects observed in the current study, we probed the uptake of glucose by C.reinhardtii cells in our experimental conditions.…”

Section: Resultsmentioning

confidence: 99%

Modulation of TOR kinase activity inChlamydomonas reinhardtii: Effect of N-starvation and changing carbon pool

Upadhyaya

Agrawal

Gorakshakar

et al. 2018

Preprint

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Target Of Rapamycin (TOR) kinase is a sensor as well as a central integrator of internal and external metabolic cues. The upstream and downstream signals of this kinase are very well characterized in animals. However, in algae, higher plants and other photosynthetic organisms, the components of the TOR kinasesignaling are yet to be characterized. Here, we establish an assay system to study TOR kinase activity in C.reinhardtii using the phosphorylation status of its downstream target, CrS6K. We further use this assay to monitor TOR kinase activity under various physiological states such as photoautotrophy, heterotrophy, mixotrophy and nitrogen starvation. We observe that autotrophy in light (and not in dark) leads to TOR kinase attenuation during N starvation while the same is not observed in mixotrophy. Importantly, we show that the external carbon source glucose is sensed and uptaken by C.reinhardtii cells only in the presence of light and not in the dark. And such exogenously added glucose, as the photoassimilate carbon mimic, results in enhanced production of ROS, induction of autophagy and concomitant drop in TOR kinase activity, creating Nstarvation-like cellular state even in N+ conditions. Interestingly, dose dependent addition of glucose revealed TOR kinase activation in low glucose regime (ROS independent) followed by attenuation of TOR kinase (ROS dependent) at high glucose levels.

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“…The photosynthetic efficiency also correlates with the Chl a/b ratio as the chlorophyll a is the primary light harvesting pigment and chlorophyll b is the accessory pigment. Positive and higher chl a/b ratio symbolizes the effective photosynthetic activity and light conversion efficiency (Karpagam et al, 2015;Li et al, 2015). CH and CC showed ratio above 2, whereas, AI and HY showed ratio in between 1 and 2 correlating to the biomass productivity and quantum yields.…”

Section: Photosynthetic Efficiency Vs Co 2 Biofixationmentioning

confidence: 99%

Photosynthetic and Lipogenic Response Under Elevated CO2 and H2 Conditions—High Carbon Uptake and Fatty Acids Unsaturation

Butti

Mohan

2018

Front. Energy Res.

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Microalgae are most versatile organisms having ability to grow under diverse conditions and utilize both organic and inorganic carbon sources. Microalgal photosynthesis can be employed to transform carbon dioxide (CO 2 ) into essential bioactives and photofuels. In this study, microalgal growth under modified headspace gas compositions which are CO 2 + H 2 (1:1), CO 2 , H 2 and Air was evaluated to determine the photosynthetic efficiency and bioactives production. A marked enhancement was observed in quantum yield (Fv/Fm: 0.77) and CO 2 biosequestration rate (0.39 g.L −1. d −1 ) under CO 2 + H 2 headspace gas condition as compared to the other experimental variations. The chemoselective functioning of Rubisco under varying gas concentrations was determined, where elevated CO 2 conditions negate the oxygenase activity under a high CO 2 /O 2 ratio which enabled higher CO 2 sequestration. The enhanced CO 2 sequestration and altered redox conditions through H 2 addition under the monophasic operation have also led to a higher average degree of unsaturation (DU) and carbon chain length (CCL) of the fatty acids produced. This study provides an approach to augment photosynthetic efficiency and lipogenesis through non-genetic modifications along with the feasibility to cultivate microalgae in integration with industrial flue gases. Exploiting the true potential of microalgae would provide sustenance from climatic changes and environmental pollution concurrently producing biobased products analogous to the fossil-derived products.

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Enhancement of lipid production and fatty acid profiling in Chlamydomonas reinhardtii, CC1010 for biodiesel production

Cited by 66 publications

References 34 publications

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

Modulation of TOR kinase activity inChlamydomonas reinhardtii: Effect of N-starvation and changing carbon pool

Photosynthetic and Lipogenic Response Under Elevated CO2 and H2 Conditions—High Carbon Uptake and Fatty Acids Unsaturation

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