Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production

Brennan, Liam; Blanco, Alfonso; Mostaert, Anika S.; Owende, Philip

doi:10.1016/j.mimet.2012.03.020

Cited by 55 publications

(67 citation statements)

References 27 publications

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“…The fluorescence peaked after 5 minutes and was kept constant for 30 minutes consecutive readings (Figure 3.3A). Such findings are in agreement with values reported elsewhere 24,51 . The interval of 30 minutes was considered enough for average sorting gates (2-10 minutes) and longer sorting periods (if very narrow sorting gates are selected, 20 -30 minutes).…”

Section: Resultssupporting

confidence: 94%

“…Results showed optimal concentrations for Nile red (NR, 0.8 µg ml -1 ) and assistant/carrier (glycerol, 125 mg ml -1 ) close to values reported by 24 . Since the cellular viability of microalgae may be affected by the toxicity of DMSO 24 , glycerol was chosen as carrier due to its low toxicity and previous applicability to fluorescence methods with microalgae 10,51 . However, the retaining time of the NR fluorescence signal started to decrease substantially after 3 minutes in the stressed cells (Figure 3.2, p < 0.001).…”

Section: Discussionmentioning

confidence: 99%

“…High throughput cell sorting can be done by coupling the fluorescence detection of intracellular lipid bodies (LB) with flow cytometry (FC) to carry out Fluorescence Assisted Cell Sorting (FACS) 10,24,51,52 . This technique allows the selection of a certain profile and the use of it to sort targeted cells, which can be grown and be used to establish a new cell lineage 10 .…”

Section: Introductionmentioning

confidence: 99%

“…However, the ultimate inadequacy is its limited photo stability resulting in signal which is not constant in time. The application of BODIPY 505/515 (BP) probe in microalgae has become much more popular over the last few years 21,24,51,52,[57][58][59] . BODIPY 505/515 (4,4-Difluoro-1,3,5,7-Tetramethyl-4-Bora-3a-Diaza-sIndacene) has a high oil/water coefficient, which allows it to infuse membranes and makes the labelling of cell components faster than NR.…”

Section: Introductionmentioning

confidence: 99%

“…BODIPY 505/515 (4,4-Difluoro-1,3,5,7-Tetramethyl-4-Bora-3a-Diaza-sIndacene) has a high oil/water coefficient, which allows it to infuse membranes and makes the labelling of cell components faster than NR. It is excited by a blue 488 nm laser, with maximum emission in the green spectrum at 515 nm, which makes it spectrally distinguishable from algal chlorophyll spectra 51,52 .…”

Section: Introductionmentioning

confidence: 99%

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The fatter the better : selecting microalgae cells for outdoor lipid production

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Current developments in high‐throughput analysis for microalgae cellular contents

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Microalgae have emerged as one of the most promising feedstocks for biofuels and bio-based chemical production. However, due to the lack of effective tools enabling rapid and high-throughput analysis of the content of microalgae biomass, the efficiency of screening and identification of microalgae with desired functional components from the natural environment is usually quite low. Moreover, the real-time monitoring of the production of target components from microalgae is also difficult. Recently, research efforts focusing on overcoming this limitation have started. In this review, the recent development of high-throughput methods for analyzing microalgae cellular contents is summarized. The future prospects and impacts of these detection methods in microalgae-related processing and industries are also addressed.

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Lipid quantification techniques for screening oleaginous species of microalgae for biofuel production

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This review examines the available literature on quantifying lipids in microalgae suitable for biofuels research. It discusses their advantages and disadvantages, their prevalence in the literature, and draws conclusions about the best way to approach choosing a suitable lipid measurement technique for microalgal biofuels research. We conclude that the method must be chosen based on the following key criteria: (1) the level of detail required in the results, and (2) the amount of biomass that can be spared for the assay. This review establishes that no method can be used as a "golden standard" for all microalgae. However, we present a systematic decision chart to choose the best measurement method or combination of methods to provide a guide to those wishing to understand the differences between the myriad of lipid measurement techniques.Practical applications: This review will allow researchers new to the field to choose the most appropriate techniques for quantifying lipids in the microalgal species under study. The review is also intended to act as a gateway to the wider literature and will enable researchers to look in depth at a particular technique before carrying out experimental work.

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Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production

Cited by 55 publications

References 27 publications

The fatter the better : selecting microalgae cells for outdoor lipid production

The fatter the better : selecting microalgae cells for outdoor lipid production

Current developments in high‐throughput analysis for microalgae cellular contents

Lipid quantification techniques for screening oleaginous species of microalgae for biofuel production

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