Enhancement Effect of Ultrasound-Induced Microbubble Cavitation on Branched Polyethylenimine-Mediated VEGF165 Transfection With Varied N/P Ratio

Zhang, Chunbing; Cao, Hui-Lin; Li, Qian; Tu, Juan; Guo, Xiaojun; Liu, Zheng; Zhang, Dong

doi:10.1016/j.ultrasmedbio.2012.08.025

Cited by 30 publications

(32 citation statements)

References 40 publications

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“…Further, with the introduction of microbubble-assisted ultrasound, a phenomenon called sonoporation has been successfully applied to deliver drugs and genes in vitro and in vivo in a variety of therapeutic application (Ferrara et al 2007;Escoffre et al 2011;Delalande et al 2013). In addition, ''Cavitation'' (139; 2.9 %) in author keywords steadily ranked in the top 11 during all four 6-year periods, which illustrates that ultrasound-induced microbubble cavitation has been an on-going concern (Doida et al 1998;Postema et al 2004;Zhang et al 2013). Moreover, the investigation of inertial cavitation in micro-tunnels has significant implications for the development of therapeutic ultrasound applications such as ultrasound-mediated drug and gene delivery (Sassaroli and Hynynen 2007).…”

Section: Author Abstractmentioning

confidence: 99%

A bibliometric analysis of micro/nano-bubble related research: current trends, present application, and future prospects

Zheng

Wang

et al. 2016

Scientometrics

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With great versatile characteristics, micro/nano-bubble related research have attracted much attention due to their extensive applications in the last half century.Researchers not merely focus on their physi-chemical properties, but also aim at their wellcontrolled generation methods and potential adhibition field. It can be expected that the future prospects of micro/nano-bubble related research will be tremendous and that there will be even more to be explored. In this case study, a bibliometric analysis was conducted to evaluate micro/nano-bubble related research from 1991 to 2014, based on the Science Citation Index EXPANDED database. The Ultrasound in Medicine and Biology with the highest h-index of 56 is the leading journal in this field, publishing 6.9 % of articles over this period, followed by Langmuir and Journal of the Acoustical Society of America. USA and the Univ Toronto, Canada were the most productive country and institution, respectively, while the USA, was the most internationally collaborative and had the highest hindex (111) of all countries. A new method named ''word cluster analysis'' was successfully applied to trace the research hotspots. Innovation in detection means and novel pathways for medical applications via micro/nano-bubble is considered to relate to the Electronic supplementary material The online version of this article

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Section: Author Abstractmentioning

confidence: 99%

A bibliometric analysis of micro/nano-bubble related research: current trends, present application, and future prospects

Zheng

Wang

et al. 2016

Scientometrics

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“…In order to enhance the efficiency of transfection, UTMD may be combined with other transfection methods. UTMD has been shown to enhance liposomal-, PEIand adeno-associated virus-mediated gene transfer in vitro and in vivo (16)(17)(18). To the best of our knowledge there is no information on UTMD-mediated non-viral vector transfection of RPE cells.…”

Section: Discussionmentioning

confidence: 99%

“…pDNA or siRNA are degraded enzymatically in the body, and UTMD remains to have a low proficiency in promoting transfection (16)(17)(18). In order to enhance the efficiency of transfection, UTMD may be combined with other transfection methods.…”

Section: Discussionmentioning

confidence: 99%

Recombinant adeno-associated virus-, polyethylenimine/plasmid- and lipofectamine/carboxyfluorescein-labeled small interfering RNA-based transfection in retinal pigment epithelial cells with ultrasound and/or SonoVue

Liu

Wan

2015

Molecular Medicine Reports

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The present study was conducted to investigate the efficacy and safety of ultrasound (US)‑mediated transfection of the type 2 recombinant adeno‑associated virus (AAV) vectors encoding the enhanced green fluorescent protein (EGFP) gene (rAAV), polyethylenimine (PEI)/plasmid EGFP‑N1 (pDNA) or lipofectamine (L)/carboxyfluorescein (FAM)‑labeled small interfering RNA (siRNA) in the human ARPE‑19 retinal pigment epithelial (RPE) cell line, with or without the addition of SonoVue. Cultured RPE cells were exposed to US, with or without SonoVue under different conditions, including variation in the intensity and duration of treatment, and the dose of microbubbles. The effects of ultrasound‑targeted microbubble destruction (UTMD) on the structure of pDNA and the transfection ability of rAAV, PEI/pDNA and L/siRNA were also evaluated. Furthermore, the effect of UTMD on RPE cells was evaluated at 0 and 24 h following UTMD. US‑mediated transfection (USMT) significantly increased L/siRNA transfection efficiency, as measured by the transgene expression per cell and the percentage of transfected cells. UTMD significantly increased rAAV and PEI/pDNA transfer to RPE cells. UTMD‑mediated rAAV or PEI/pDNA delivery was more effective than USMT‑mediated delivery of siRNA. Evaluating cell viability at 24 h post‑UTMD provided more valuable information than immediate evaluation following UTMD. Furthermore, there was minimal cytotoxicity and minimal change to the structure of pDNA under the optimal parameters. UTMD/US may be of use in enhancing rAAV, PEI/pDNA and L/siRNA transgene expression of ARPE‑19 cells in vitro. Studies on the transfection of different nucleotides (such as pDNA and siRNA) and different types of vectors (chemical and biological) mediated by UTMD may provide useful information to guide future in vivo and transfection studies.

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“…Transfection efficiency is one of very important factors. At present, many researchers have studied the transfection parameters (such as microbubble concentration and composition, the ultrasonic intensity, duty cycle, plasmid DNA concentration, and DNA phosphate ratio) which can dramatically affect the transfection efficiency [34, 53, 54]. And they found that a higher transfection efficiency and a lower cell death could be simultaneously obtained by means of optimizing parameters [55].…”

Section: Summary and Prospectmentioning

confidence: 99%

New Progress in Angiogenesis Therapy of Cardiovascular Disease by Ultrasound Targeted Microbubble Destruction

Liao

Chen

Wáng

et al. 2014

BioMed Research International

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Angiogenesis plays a vital part in the pathogenesis and treatment of cardiovascular disease and has become one of the hotspots that are being discussed in the past decades. At present, the promising angiogenesis therapies are gene therapy and stem cell therapy. Besides, a series of studies have shown that the ultrasound targeted microbubble destruction (UTMD) was a novel gene delivery system, due to its advantages of noninvasiveness, low immunogenicity and toxicity, repeatability and temporal and spatial target specificity; UTMD has also been used for angiogenesis therapy of cardiovascular disease. In this review, we mainly discuss the combination of UTMD and gene therapy or stem cell therapy which is applied in angiogenesis therapy in recent researches, and outline the future challenges and good prospects of these approaches.

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Enhancement Effect of Ultrasound-Induced Microbubble Cavitation on Branched Polyethylenimine-Mediated VEGF165 Transfection With Varied N/P Ratio

Cited by 30 publications

References 40 publications

A bibliometric analysis of micro/nano-bubble related research: current trends, present application, and future prospects

A bibliometric analysis of micro/nano-bubble related research: current trends, present application, and future prospects

Recombinant adeno-associated virus-, polyethylenimine/plasmid- and lipofectamine/carboxyfluorescein-labeled small interfering RNA-based transfection in retinal pigment epithelial cells with ultrasound and/or SonoVue

New Progress in Angiogenesis Therapy of Cardiovascular Disease by Ultrasound Targeted Microbubble Destruction

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