Enhanced protective immune response to PCV2 adenovirus vaccine by fusion expression of Cap protein with InvC in pigs

Abstract

The major immunogenic protein capsid (Cap) of porcine circovirus type 2 (PCV2) is critical to induce neutralizing antibodies and protective immune response against PCV2 infection. This study was conducted to investigate the immune response of recombinant adenovirus expressing PCV2b Cap and C-terminal domain of Yersinia pseudotuberculosis invasin (Cap-InvC) fusion protein in pigs. The recombinant adenovirus rAd-Cap-InvC, rAd-Cap and rAd were generated and used to immunize pigs. The phosphate-buffered saline was used as negative control. The specific antibodies levels in rAd-Cap-InvC and ZJ/C-strain vaccine groups were higher than that of rAd-Cap group ( p < 0.05), and the neutralization antibody titer in rAd-Cap-InvC group was significantly higher than those of other groups during 21–42 days post-immunization (DPI). Moreover, lymphocyte proliferative level, interferon-γ and interleukin-13 levels in rAd-Cap-InvC group were increased compared to rAd-Cap group ( p < 0.05). After virulent challenge, viruses were not detected from the blood samples in rAd-Cap-InvC and ZJ/C-strain vaccine groups after 49 DPI. And the respiratory symptom, rectal temperature, lung lesion and lymph node lesion were minimal and similar in the ZJ/C-strain and rAd-Cap-InVC groups. In conclusion, our results demonstrated that rAd-Cap-InvC was more efficiently to stimulate the production of antibody and protect pigs from PCV2 infection. We inferred that InvC is a good candidate gene for further development and application of PCV2 genetic engineering vaccine.

Fusion Expression and Immune Effect of PCV2 Cap Protein Tandem Multiantigen Epitopes with CD154/GM-CSF

“…PCV2 capsid (Cap) protein is a viral nucleocapsid protein encoded by open reading frame 2 (ORF2) and contains specific antigenic determinants [8]. The Cap protein is the major immunogenic structural protein of PCV2 and is responsible for neutralizing antibodies and inducing humoral and cellular immunity [9][10][11]. Therefore, the Cap protein is an important target in the design of a novel PCV2 vaccine using genetic engineering technology.…”

Immunomodulatory Effects of Hydrolyzed Seawater Pearl Tablet (HSPT) on Th1/Th2 Functionality in a Mice Model of Chronic Obstructive Pulmonary Disease (COPD) Induced by Cigarette Smoke

“…e spleen is the Evidence-Based Complementary and Alternative Medicine largest immune organ in the body and contains a large number of lymphocytes. Lymphocyte proliferation is an important index of the cellular immune response [54]. Based on a previous finding that HSPT could regulate the numbers of T cells to improve the body's immunity [25], we addressed whether HSPT mediates splenic lymphocyte proliferation induced by CS exposure.…”

Porcine Circovirus Type 2 Vaccines: Commercial Application and Research Advances

“…The recombinant adenovirus vaccine demonstrates a potent immune response and superior protection and is expected to become a candidate vaccine for PCVAD [75]. In addition, studies have shown that the Yersinia pseudotuberculosis invasin (Inv) protein can enhance the immune response and can be used to construct a recombinant adenovirus containing the PCV2b Cap gene and InvC gene, which has a good immune response [76].…”