Enhanced production of prodigiosin by Serratia marcescens FZSF02 in the form of pigment pellets

Chao, Lin; Jia, Xiaoling; Fang, Yanwen; Chen, Longjun; Zhang, Hui; Lin, Renping; Chen, Jichen

doi:10.1016/j.ejbt.2019.04.007

Cited by 61 publications

(54 citation statements)

References 27 publications

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“…With the medium containing 0.94% α-chitin + 0.56% Casein, PG produced by S. marcescens TKU011 strain reached 335 (mg/100mL). However, two previous studies reported that with 2.0% sesame seed [26] and the medium containing 6.97 g/L of peanut powder, 11.29 mL/L of olive oil and 16.02 g/L of beef extract [33] even reached the PG yield of 1668 and 1362.2 mg/100mL, respectively. These are cases with 4.979-fold and 4.066-fold higher yield than PG yield produced by S. marcescens TKU011 in this study.…”

Section: New Records Of Marine α-Chitin As the Potential Carbon Sourcmentioning

confidence: 89%

“…These are cases with 4.979-fold and 4.066-fold higher yield than PG yield produced by S. marcescens TKU011 in this study. In these above cited reports [26,33], the PGproducing bacteria may be unique strains and the qualification of PG in these studies were not described in detail. For the comparison of the PG producing by different S. marcescens strains, a total four strains including S. marcescens TKU011, S. marcescens CC17, S. marcescens TNU01, and S. marcescens TNU02 were conducted for fermentation.…”

Section: New Records Of Marine α-Chitin As the Potential Carbon Sourcmentioning

confidence: 99%

“…These are cases with 4.979-fold and 4.066-fold higher yield than PG yield produced by S. marcescens TKU011 in this study. In these above cited reports [26,33], the PG-producing bacteria may be unique strains and the qualification of PG in these studies were not described in detail.…”

Section: New Records Of Marine α-Chitin As the Potential Carbon Sourcmentioning

confidence: 99%

“…than β-chitin (mainly obtained from squid pens); thus, α-chitin was chosen for our further investigation. Based on the recent literature review, PG has been produced by S. marcescens with various types of carbon/nitrogen sources [9][10][11]13,14,[25][26][27][28][29][30][31][32][33]; however, very few studies report on the use of chitinous materials (squid pens) [19] as the carbon/nitrogen source for PG production; for the first time in this study, α-chitin obtained from marine resources were investigated as a potent carbon source for high scale PG production via microbial fermentation.…”

Section: New Records Of Marine α-Chitin As the Potential Carbon Sourcmentioning

confidence: 99%

“…The fermentation was performed at the conditions at 30 °C in 1 d, and then at 25 °C in next 2 d, shaking speed of 150 rpm, and a ratio volume of medium: flask of 1:2.5 (v/v). To date, PG has been produced from many carbon sources with multiple designed media [9][10][11][13][14][25][26][27][28][29][30][31][32][33]. As summarized in Table 2, the designed medium gave the PG productivity in the scale of 0 up to around 200 (mg/100mL).…”

Section: New Records Of Marine α-Chitin As the Potential Carbon Sourcmentioning

confidence: 99%

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Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin

et al. 2019

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Marine chitins (MC) have been utilized for the production of vast array of bioactive products, including chitooligomers, chitinase, chitosanase, antioxidants, anti-NO, and antidiabetic compounds. The aim of this study is the bioprocessing of MC into a potent anticancer compound, prodigiosin (PG), via microbial fermentation. This bioactive compound was produced by Serratia marcescens TKU011 with the highest yield of 4.62 mg/mL at the optimal conditions of liquid medium with initial pH of 5.65–6.15 containing 1% α-chitin, 0.6% casein, 0.05% K2HPO4, and 0.1% CaSO4. Fermentation was kept at 25 °C for 2 d. Notably, α-chitin was newly investigated as the major potential material for PG production via fermentation; the salt CaSO4 was also found to play the key role in the enhancement of PG yield of Serratia marcescens fermentation for the first time. PG was qualified and identified based on specific UV, MALDI-TOF MS analysis. In the biological activity tests, purified PG demonstrated potent anticancer activities against A549, Hep G2, MCF-7, and WiDr with the IC50 values of 0.06, 0.04, 0.04, and 0.2 µg/mL, respectively. Mytomycin C, a commercial anti-cancer compound was also tested for comparison purpose, showing weaker activity with the IC50 values of 0.11, 0.1, 0.14, and 0.15 µg/mL, respectively. As such, purified PG displayed higher 2.75-fold, 1.67-fold, and 3.25-fold efficacy than Mytomycin C against MCF-7, A549, and Hep G2, respectively. The results suggest that marine chitins are valuable sources for production of prodigiosin, a potential candidate for cancer drugs.

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Section: New Records Of Marine α-Chitin As the Potential Carbon Sourcmentioning

confidence: 89%

Section: New Records Of Marine α-Chitin As the Potential Carbon Sourcmentioning

confidence: 99%

Section: New Records Of Marine α-Chitin As the Potential Carbon Sourcmentioning

confidence: 99%

Section: New Records Of Marine α-Chitin As the Potential Carbon Sourcmentioning

confidence: 99%

Section: New Records Of Marine α-Chitin As the Potential Carbon Sourcmentioning

confidence: 99%

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Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin

et al. 2019

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Prodigiosin production from Serratia marcescens strain CSK and their antioxidant, antibacterial, cytotoxic effect and in silico study of caspase‐3 apoptotic protein

Sudhakar¹,

Shobana²,

Selvankumar³

et al. 2021

Biotech and App Biochem

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The present study emphasizes the production and optimization of prodigiosin (PG) pigment from Serratia marcescens strain CSK, which was isolated from Shevaroy Hills, Salem district, Tamil Nadu, India. The response surface methodology analysis was applied for the optimization process of PG production. The maximum production of PG (2950 mg/L) was obtained at pH 7.0 with the addition of tryptophan (4.0 g/L) and sucrose (3.0 g/L) with 60 h of incubation. Further, the PG was characterized using high‐performance liquid chromatography, Fourier‐transform infrared spectroscopy, and gas chromatography‐mass spectrometry. The purified PG exhibited strong antioxidant and antibacterial activities. Also, PG's cytotoxic effects against human breast cancer (MCF‐7) cells were observed through acridine orange‐ethidium bromide (AO‐EB) and Hoechst staining. Molecular dockingstudies revealed that PG could bind positively to the caspase‐3 (breast cancer protein 1RE1) binding site with a binding energy score of 17.37 kcal/mol. Overall, the novel PG was found to be an anticancer drug for potential applications in the pharmaceutical industry.

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High‐level production of microbial prodigiosin: A review

Han

Xiang

et al. 2021

J Basic Microbiol

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Prodigiosin is a natural red pigment derived primarily from secondary metabolites of microorganisms, especially Serratia marcescens. It can also be chemically synthesized. Prodigiosin has been proven to have antitumor, antibacterial, antimalaria, anti‐insect, antialgae, and immunosuppressive activities, and is gaining increasing important in the global market because of its great potential application value in clinical medicine development, environmental treatment, preparation of food additives, and so on. Due to the low efficiency of prodigiosin chemical synthesis, high‐level prodigiosin of production by microorganisms are necessary for prodigiosin applications. In this paper, the production of prodigiosin by microorganism in recent decades is reviewed. The methods and strategies for increasing the yield of prodigiosin are discussed from the aspects of medium composition, additives, factors affecting production conditions, strain modification, and fermentation methods.

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Enhanced production of prodigiosin by Serratia marcescens FZSF02 in the form of pigment pellets

Cited by 61 publications

References 27 publications

Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin

Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin

Prodigiosin production from Serratia marcescens strain CSK and their antioxidant, antibacterial, cytotoxic effect and in silico study of caspase‐3 apoptotic protein

High‐level production of microbial prodigiosin: A review

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