Enhanced production of pigments by addition of surfactants in submerged fermentation of <scp><i>Monascus purpureus</i> H1102</scp>

Yong-hui, Wang; Zhang, Bobo; Lü, Liping; Huang, Yan; Xu, Guowang

doi:10.1002/jsfa.6182

Cited by 68 publications

(64 citation statements)

References 16 publications

(25 reference statements)

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“…4a). It has been reported that the lipid cell membrane could be modified in the nonionic surfactant micelle solution, thereby increasing the permeability of cell membrane and the intracellular pigment export in the perstractive fermentation process (Wang et al 2013). In this study, with low pigment content in fermentation broth or mature cells, the intracellular pigments could be completely extracted into the extracellular broth (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…By using TX as the additive, an enrichment of the hydrophobic pigments occurred upon being “milked” in the artificial nonionic micelle aqueous solution to improve the pigment penetration across the cellular membrane (Hu et al 2012b; Kang et al 2013a). Further study indicated that the nonionic surfactant may modify the cell membrane lipid layer and then improve the rate of secretion of intracellular hydrophobic pigments across the cell membrane (Wang et al 2013). However, the final extracellular pigment concentration is mainly attributed to the solubilization of pigments into the nonionic surfactant micelles and then increasing the concentration gradient between the intracellular and extracellular pigments (Kang et al 2013b).…”

Section: Introductionmentioning

confidence: 99%

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Saturation effect and transmembrane conversion of Monascus pigment in nonionic surfactant aqueous solution

et al. 2017

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Extractive fermentation in a nonionic surfactant aqueous solution provides a promising and efficient method to produce Monascus pigments. The behaviour of pigment secretion during the extractive cultivation was investigated in the present work. The results revealed that the secretion of intracellular pigment was limited by its saturation concentration in the nonionic surfactant aqueous solution. The intracellular pigment was completely extracted to the outside of the cell at a low cell density and high concentration of Triton X-100 (TX) in fermentation broth; otherwise, a restriction for pigment extraction would occur. The decrement of the intracellular orange and yellow pigments was inconsistent with the increment of extracellular pigments with an increase in the TX concentration. It could be inferred that the intracellular orange pigment was converted to extracellular yellow pigment during the transmembrane secretion process, which might be attributed to the enzyme catalysis in the non-aqueous phase solution. This study helps explain the mechanism of variation of pigment characteristic and extraction capacity in extractive fermentation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Saturation effect and transmembrane conversion of Monascus pigment in nonionic surfactant aqueous solution

et al. 2017

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“…The fatty acid in cell membrane of the mycelia was extracted, purified and methylated according to the method described by Wang et al (2013). After that, the sample dissolved in the n -hexane was collected for GC–MS analysis, using an Agilent 6890 GC (Agilent, Santa Clara, CA, USA) coupled to an Agilent 5973 mass selective detector (MSD) (Agilent, Santa Clara, CA, USA), equipped with a HP-5MS column (5% Phenyl Methyl Silox, 30 m–0.25 mm id 0.25 μm film thickness, Agilent, Santa Clara, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Glutamic acid could promote the monacolin K production by regulating the permeability of Monascus mycelium and then the secretion of monacolin K was promoted without feedback inhibition from intracellular product (Zhang et al 2017). The permeability and fluidity of cell membrane depended on the saturability of the containing fatty acid (Wang et al 2013). As high carbon source but low oxidoreduction potential (ORP) could benefit the production of extracellular water-soluble yellow pigments with Monascus ruber CGMCC 10910 (Wang et al 2017), multifaceted mechanisms of high glucose stress that had impacted the metabolism and secretion of Monascus yellow pigments should be further investigated.…”

Section: Introductionmentioning

confidence: 99%

Metabolism and secretion of yellow pigment under high glucose stress with Monascus ruber

et al. 2017

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The biosynthesis of microbial secondary metabolites is induced by a wide range of environmental stresses. In this study, submerged fermentation of Monascus yellow pigments by Monascus ruber CGMCC 10910 under high glucose stress was investigated. The increase of lipid content was the major contributor to the increase of dry cell weight (DCW), and the lipid-free DCW was only slightly changed under high glucose stress, which benefited the accumulation of intracellular hydrophobic pigments. The fatty acid composition analysis in Monascus cell membranes showed that high glucose stress significantly increased the ratio of unsaturated/saturated fatty acid and the index of unsaturated fatty acid (IUFA) value, which would improve the fluidity and permeability of the cell membrane. As a consequence, high glucose stress increased extracellular yellow pigments production by enhancing secretion and trans-membrane conversion of intracellular pigments to the broth. The total yield of extracellular and intracellular yellow pigments per unit of lipid-free DCW increased by 94.86 and 26.31% under high glucose stress compared to conventional fermentation, respectively. A real-time quantitative PCR analysis revealed that the expression of the pigment biosynthetic gene cluster was up-regulated under high glucose stress. The gene mppE, which is associated with yellow pigment biosynthesis, was significantly up-regulated. These results indicated that high glucose stress can shift the Monascus pigment biosynthesis pathway to accumulate yellow pigments and lead to a high yield of both extracellular and intracellular yellow pigments. These findings have potential application in commercial Monascus yellow pigment production.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0382-5) contains supplementary material, which is available to authorized users.

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“…Monascus pigment is a natural colorant with many activities (e.g. antibacterial and antioxidant), and it has been considered an ideal replacement for synthetic pigments . Pigment production cannot be further increased using the current liquid fermentation methods, primarily because of feedback inhibition and downstream purification .…”

Section: Introductionmentioning

confidence: 99%

Effects of nonionic surfactants on pigment excretion and cell morphology in extractive fermentation of Monascus sp. NJ1

et al. 2018

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Enhanced production of pigments by addition of surfactants in submerged fermentation of Monascus purpureus H1102

Abstract: The addition of surfactant Triton X-100 could greatly enhance the production of pigment. It was suggested that Triton X-100 facilitated the secretion of intracellular pigment and therefore enhanced pigment production accordingly.

Cited by 68 publications

References 16 publications

Saturation effect and transmembrane conversion of Monascus pigment in nonionic surfactant aqueous solution

Saturation effect and transmembrane conversion of Monascus pigment in nonionic surfactant aqueous solution

Metabolism and secretion of yellow pigment under high glucose stress with Monascus ruber

Effects of nonionic surfactants on pigment excretion and cell morphology in extractive fermentation of Monascus sp. NJ1

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