Enhanced Expression of Aggrus (T1alpha/Podoplanin), a Platelet-Aggregation-Inducing Factor in Lung Squamous Cell Carcinoma

Abstract

Aggrus (T1α/podoplanin, known as a specific marker for type I alveolar cells or lymphatic endothelial cells) is a transmembrane sialoglycoprotein that aggregates platelets. Previously, we showed that upregulated expression of Aggrus occurs in colorectal tumors or testicular tumors and could be associated with platelet-aggregating activity and metastatic ability. In testicular tumors, Aggrus is specifically expressed in seminoma. The present study investigates Aggrus expression in human primary lung cancer tissues of different types. Microarray analysis demonstrated that aggrus was significantly expressed in squamous cell carcinoma (10/15; 66.7%). Immunohistochemical analysis also showed that the incidence of positive staining in sections of squamous cell carcinoma (7/8; 87.5%) was higher than that in adenocarcinoma (2/13; 15.4%). Furthermore, Aggrus expression was detected in a squamous cell carcinoma cell line, NCI-H226, by real-time PCR. These findings indicated that overexpression of Aggrus occurred in squamous cell carcinoma of the lung. Therefore, Aggrus could be a useful diagnostic marker for squamous cell carcinoma of the lung.

A critical role of platelet TGF-β release in podoplanin-mediated tumour invasion and metastasis

“…To elucidate the role of podoplanin-mediated tumour-induced platelet aggregation in tumour behaviour, we first investigated podoplanin-positive tumour cell lines for potential to induce platelet aggregation. Consistent with previous reports, podoplanin expression was detected in bladder SCC cell line UM-UC-5 26 and malignant pleural mesothelioma cell line NCI-H226 (referred to as H226 from hereon) 21 , but not in lung adenocarcinoma cell line A549 40 ( Fig. 1a , Supplementary Fig.…”

A critical role of platelet TGF-β release in podoplanin-mediated tumour invasion and metastasis

“…To elucidate the role of podoplanin-mediated tumour-induced platelet aggregation in tumour behaviour, we first investigated podoplanin-positive tumour cell lines for potential to induce platelet aggregation. Consistent with previous reports, podoplanin expression was detected in bladder SCC cell line UM-UC-5 26 and malignant pleural mesothelioma cell line NCI-H226 (referred to as H226 from hereon) 21 , but not in lung adenocarcinoma cell line A549 40 ( Fig. 1a , Supplementary Fig.…”

Expression of Aggrus/podoplanin in bladder cancer and its role in pulmonary metastasis

“…). Several groups have reported Aggrus upregulation in SCC of lung, esophagus and head and neck . Thus, our finding is consistent with those of the above mentioned reports.…”

Podoplanin expression by cancer-associated fibroblasts predicts poor outcome in invasive ductal breast carcinoma

“…It should be stressed that, in our study, only 2/117 cases showed podoplanin immunoreactivity in cancer cells. These results were confirmed by PCR analysis of the studied breast cancer cell lines, and are consistent with previous reports by other authors showing that adenocarcinomas rarely express podoplanin 8,20,33,34 …”

A critical role of platelet TGF-β release in podoplanin-mediated tumour invasion and metastasis

“…To elucidate the role of podoplanin-mediated tumour-induced platelet aggregation in tumour behaviour, we first investigated podoplanin-positive tumour cell lines for potential to induce platelet aggregation. Consistent with previous reports, podoplanin expression was detected in bladder SCC cell line UM-UC-5 26 and malignant pleural mesothelioma cell line NCI-H226 (referred to as H226 from hereon) 21 , but not in lung adenocarcinoma cell line A549 40 ( Fig. 1a , Supplementary Fig.…”

Expression of Aggrus/podoplanin in bladder cancer and its role in pulmonary metastasis

“…). Several groups have reported Aggrus upregulation in SCC of lung, esophagus and head and neck . Thus, our finding is consistent with those of the above mentioned reports.…”

Podoplanin expression by cancer-associated fibroblasts predicts poor outcome in invasive ductal breast carcinoma

“…It should be stressed that, in our study, only 2/117 cases showed podoplanin immunoreactivity in cancer cells. These results were confirmed by PCR analysis of the studied breast cancer cell lines, and are consistent with previous reports by other authors showing that adenocarcinomas rarely express podoplanin 8,20,33,34 …”

A critical role of platelet TGF-β release in podoplanin-mediated tumour invasion and metastasis

“…To elucidate the role of podoplanin-mediated tumour-induced platelet aggregation in tumour behaviour, we first investigated podoplanin-positive tumour cell lines for potential to induce platelet aggregation. Consistent with previous reports, podoplanin expression was detected in bladder SCC cell line UM-UC-5 26 and malignant pleural mesothelioma cell line NCI-H226 (referred to as H226 from hereon) 21 , but not in lung adenocarcinoma cell line A549 40 ( Fig. 1a , Supplementary Fig.…”

Expression of Aggrus/podoplanin in bladder cancer and its role in pulmonary metastasis

“…). Several groups have reported Aggrus upregulation in SCC of lung, esophagus and head and neck . Thus, our finding is consistent with those of the above mentioned reports.…”

Podoplanin expression by cancer-associated fibroblasts predicts poor outcome in invasive ductal breast carcinoma

“…It should be stressed that, in our study, only 2/117 cases showed podoplanin immunoreactivity in cancer cells. These results were confirmed by PCR analysis of the studied breast cancer cell lines, and are consistent with previous reports by other authors showing that adenocarcinomas rarely express podoplanin 8,20,33,34 …”