Enhanced Cutaneous Wound Healing In Vivo by Standardized Crude Extract of Poincianella pluviosa

Abstract

Wound healing is a complex process that involves several biological events, and a delay in this process may cause economic and social problems for the patient. The search continues for new alternative treatments to aid healing, including the use of herbal medicines. Members of the genus Caesalpinia are used in traditional medicine to treat wounds. The related species Poincianella pluviosa (DC.) L.P. Queiroz increases the cell viability of keratinocytes and fibroblasts and stimulates the proliferation of keratinocytes in vitro. The crude extract (CE) from bark of P. pluviosa was evaluated in the wound-healing process in vivo, to validate the traditional use and the in vitro activity. Standardized CE was incorporated into a gel and applied on cutaneous wounds (TCEG) and compared with the formulation without CE (Control) for 4, 7, 10, or 14 days of treatment. The effects of the CE on wound re-epithelialization; cell proliferation; permeation, using photoacoustic spectroscopy (PAS); and proteins, including vascular endothelial growth factor (VEGF), superoxide dismutase 2 (SOD-2) and cyclooxygenase 2 (COX-2) were evaluated. The TCEG stimulated the migration of keratinocytes at day 4 and proliferation on the following days, with a high concentration of cells in metaphase at 7 days. Type I collagen formed more rapidly in the TCEG. PAS showed that the CE had permeated through the skin. TCEG stimulated VEGF at day 4 and SOD-2 and COX-2 at day 7. The results suggest that the CE promoted the regulation of proteins and helped to accelerate the processes involved in healing, promoting early angiogenesis. This led to an increase in the re-epithelialized surface, with significant mitotic activity. Maturation of collagen fibers was also enhanced, which may affect the resistance of the extracellular matrix. PAS indicated a correlation between the rate of diffusion and biological events during the healing process. The CE from P. pluviosa appears promising as an aid in healing.

HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms

“…Our study demonstrated new vessels appear as early as three days after wounding and staining for VEGF was most prominent in keratinocytes at seven days post-surgery, consistent with VEGF expression results of previous reports [34,35,36]. HMGB1 administration restored the blood flow recovery in the ischemic muscle of diabetic mice is associated with the increased expression of VEGF [37].…”

“…Attempts have been made to repair and restore destroyed periodontal tissues, including the use of particular bioactive substances or surgical bone grafts [20,34]. Nowadays, conventional therapies are poorly effective on complex chronic ulcers and tissue lesions occurring in specific diseases, like diabetes.…”

Leaves from the Tree Poincianella pluviosa as a Renewable Source of Antiplasmodial Compounds against Chloroquine-Resistant Plasmodium falciparum

“…The stem bark from the tree presents antidiarrheal, wound healing and antimalarial therapeutic properties. [14][15][16] More specifically, Tacana Indians living in the Amazonian Bolivian forest use decoction of the stem bark from P. pluviosa for diarrhea and dysentery. Phenolic compounds and terpenoids were reported in flowers and stem bark from P. pluviosa.…”

“…Phenolic compounds and terpenoids were reported in flowers and stem bark from P. pluviosa. [16][17][18] Taking into account the medicinal aspects of P. pluviosa, this study aimed to evaluate the antimalarial potential of its leaves. Leaves were chosen because of the abundance of these plant organs and their renewable characteristics in comparison with stem bark whose over-collection can be a threat to the integrity of the species, possibly leading to its extinction.…”

Assessment of the efficacy and tolerance of an innovative regenerative serum on cutaneous regeneration, following fractional laser procedure using Erbium:YAG

“…It involves several biological events, including vascular and cellular changes, epithelial proliferation, collagen synthesis and deposition, fibroblast proliferation, and wound contraction . The optimal outcome of cutaneous wound healing involves regeneration of the normal architecture and function of the skin .…”