Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)

Lin‐Wang, Kui; McGhie, Tony K.; Wang, Mindy; Liu, Yuhui; Warren, Ben; Storey, Roy; Espley, Richard V.; Allan, Andrew C.

doi:10.3389/fpls.2014.00651

Cited by 132 publications

(143 citation statements)

References 48 publications

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“…A similar result also was observed for strawberry FaMYB1 . In fruit, FaMYB1 showed the highest transcription level at the red ripe stage when anthocyanin accumulation reached a peak (Aharoni et al ., ; Lin‐Wang et al ., ). These results suggest that the PpMYB18‐ like genes are likely to be induced by anthocyanin‐ and/or PA‐related activators, thus providing negative feedback regulation to MBW complexes.…”

Section: Discussionmentioning

confidence: 97%

Activator‐type R2R3‐MYB genes induce a repressor‐type R2R3‐MYB gene to balance anthocyanin and proanthocyanidin accumulation

et al. 2018

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Anthocyanin and proanthocyanidin (PA) accumulation is regulated by both myeloblastosis (MYB) activators and repressors, but little information is available on hierarchical interactions between the positive and negative regulators. Here, we report on a R2R3-MYB repressor in peach, designated PpMYB18, which acts as a negative regulator of anthocyanin and PA accumulation. PpMYB18 can be activated by both anthocyanin- and PA-related MYB activators, and is expressed both at fruit ripening and juvenile stages when anthocyanins or PAs, respectively, are being synthesized. The PpMYB18 protein competes with MYB activators for binding to basic Helix Loop Helixes (bHLHs), which develops a fine-tuning regulatory loop to balance PA and anthocyanin accumulation. In addition, the bHLH binding motif in the R3 domain and the C1 and C2 repression motifs in the C-terminus of PpMYB18 both confer repressive activity of PpMYB18. Our study also demonstrates a modifying negative feedback loop, which prevents cells from excess accumulation of anthocyanin and PAs, and serves as a model for balancing secondary metabolite accumulation at the transcriptional level.

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Section: Discussionmentioning

confidence: 97%

Activator‐type R2R3‐MYB genes induce a repressor‐type R2R3‐MYB gene to balance anthocyanin and proanthocyanidin accumulation

et al. 2018

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“…18,19,21 In the prior studies, expression of multiple early- and late-regulated biosynthesis genes in the flavonoid/phenylpropanoid pathway was reduced in transgenic MYB10 RNAi or yellow-fruited strawberry. In contrast, our RNA-seq data showed a limited number of biosynthesis genes whose expression was reduced in the yellow-fruited strawberry.…”

Section: Discussionmentioning

confidence: 99%

An eFP browser for visualizing strawberry fruit and flower transcriptomes

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et al. 2017

Hortic Res

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Wild strawberry Fragaria vesca is emerging as an important model system for the cultivated strawberry due to its diploid genome and availability of extensive transcriptome data and a range of molecular genetic tools. Being able to better utilize these tools, especially the transcriptome data, will greatly facilitate research progress in strawberry and other Rosaceae fruit crops. The electronic fluorescent pictograph (eFP) software is a useful and popular tool to display transcriptome data visually, and is widely used in other model organisms including Arabidopsis and mouse. Here we applied eFP to display wild strawberry RNA sequencing (RNA-seq) data from 42 different tissues and stages, including various flower and fruit developmental stages. In addition, we generated eight additional RNA-seq data sets to represent tissues from ripening-stage receptacle fruit from yellow-colored and red-colored wild strawberry varieties. Differential gene expression analysis between these eight data sets provides additional information for understanding fruit-quality traits. Together, this work greatly facilitates the utility of the extensive transcriptome data for investigating strawberry flower and fruit development as well as fruit-quality traits.

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“…Peach and octoploid strawberry ( Fragaria × ananassa ) genome encodes two and four copies of Ans gene, respectively (Almeida et al, 2007; Ravaglia et al, 2013). Only one gene copy has been described in diploid strawberry ( F. vesca ) and apple (Takos et al, 2006; Lin-Wang et al, 2014). …”

Section: Discussionmentioning

confidence: 99%

Nonsense Mutation Inside Anthocyanidin Synthase Gene Controls Pigmentation in Yellow Raspberry (Rubus idaeus L.)

et al. 2016

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Yellow raspberry fruits have reduced anthocyanin contents and offer unique possibility to study the genetics of pigment biosynthesis in this important soft fruit. Anthocyanidin synthase (Ans) catalyzes the conversion of leucoanthocyanidin to anthocyanidin, a key committed step in biosynthesis of anthocyanins. Molecular analysis of the Ans gene enabled to identify an inactive ans allele in a yellow fruit raspberry (“Anne”). A 5 bp insertion in the coding region was identified and designated as ans+5. The insertion creates a premature stop codon resulting in a truncated protein of 264 amino acids, compared to 414 amino acids wild-type ANS protein. This mutation leads to loss of function of the encoded protein that might also result in transcriptional downregulation of Ans gene as a secondary effect, i.e., nonsense-mediated mRNA decay. Further, this mutation results in loss of visible and detectable anthocyanin pigments. Functional characterization of raspberry Ans/ans alleles via complementation experiments in the Arabidopsis thaliana ldox mutant supports the inactivity of encoded protein through ans+5 and explains the proposed block in the anthocyanin biosynthetic pathway in raspberry. Taken together, our data shows that the mutation inside Ans gene in raspberry is responsible for yellow fruit phenotypes.

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Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)

Cited by 132 publications

References 48 publications

Activator‐type R2R3‐MYB genes induce a repressor‐type R2R3‐MYB gene to balance anthocyanin and proanthocyanidin accumulation

Activator‐type R2R3‐MYB genes induce a repressor‐type R2R3‐MYB gene to balance anthocyanin and proanthocyanidin accumulation

An eFP browser for visualizing strawberry fruit and flower transcriptomes

Nonsense Mutation Inside Anthocyanidin Synthase Gene Controls Pigmentation in Yellow Raspberry (Rubus idaeus L.)

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