Engineering Smart Hybrid Tissues with Built-In Electronics

Feiner, Ron

doi:10.1016/j.isci.2020.100833

Cited by 17 publications

(24 citation statements)

References 99 publications

(132 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Not surprisingly, our research has been gradually fueling the vision of a hybrid platform capable of fabricating these sensors, actuators and living skin tissues simultaneously. Although this concept would unleash an all-printed, ready-to-implant, dermo-epidermal skin substitutes with built-in electronics (able to report both cell´s status and functions and activate regenerative outcomes), as cyborg tissues [ 4 ], this endeavor remains challenging.…”

Section: Introductionmentioning

confidence: 99%

Real-Time Impedance Monitoring of Epithelial Cultures with Inkjet-Printed Interdigitated-Electrode Sensors

Mojena-Medina

Hubl²,

Bäuscher

et al. 2020

Sensors

View full text Add to dashboard Cite

From electronic devices to large-area electronics, from individual cells to skin substitutes, printing techniques are providing compelling applications in wide-ranging fields. Research has thus fueled the vision of a hybrid, printing platform to fabricate sensors/electronics and living engineered tissues simultaneously. Following this interest, we have fabricated interdigitated-electrode sensors (IDEs) by inkjet printing to monitor epithelial cell cultures. We have fabricated IDEs using flexible substrates with silver nanoparticles as a conductive element and SU-8 as the passivation layer. Our sensors are cytocompatible, have a topography that simulates microgrooves of 300 µm width and ~4 µm depth, and can be reused for cellular studies without detrimental in the electrical performance. To test the inkjet-printed sensors and demonstrate their potential use for monitoring laboratory-growth skin tissues, we have developed a real-time system and monitored label-free proliferation, migration, and detachment of keratinocytes by impedance spectroscopy. We have found that variations in the impedance correlate linearly to cell densities initially seeded and that the main component influencing the total impedance is the isolated effect of the cell membranes. Results obtained show that impedance can track cellular migration over the surface of the sensors, exhibiting a linear relationship with the standard method of image processing. Our results provide a useful approach for non-destructive in-situ monitoring of processes related to both in vitro epidermal models and wound healing with low-cost ink-jetted sensors. This type of flexible sensor as well as the impedance method are promising for the envisioned hybrid technology of 3D-bioprinted smart skin substitutes with built-in electronics.

show abstract

Section: Introductionmentioning

confidence: 99%

Real-Time Impedance Monitoring of Epithelial Cultures with Inkjet-Printed Interdigitated-Electrode Sensors

Mojena-Medina

Hubl²,

Bäuscher

et al. 2020

Sensors

View full text Add to dashboard Cite

show abstract

“…The extracellular matrix (ECM) is a very dynamic meshwork of molecules, comprised of proteins and sugars (glycans), [13,73] released by neurons and glia in the interstitial space of tissues, as shown in Figure 4B. It has features that vary in size from 50 to 500 nm, [74,75] constituting 10-20% of brain volume.…”

Section: Extracellular Matrixmentioning

confidence: 99%

“…[ 94 ] Investigation into neuronal growth on poly(acrylic acid) (PAA) gel uncovered the fact that neurons successfully produce neurites, whereas glial growth was nonexistent. [ 94 ] Methods of producing stretchable substrates include metal deposition onto a material surface while it is being stretched, or serpentine layouts, [ 73 ] and as such there is opportunity to further investigate the effects of flexible substrates on neuronal growth in vivo.…”

Section: Bioinspired Designmentioning

confidence: 99%

The Future of Neuroscience: Flexible and Wireless Implantable Neural Electronics

et al. 2021

View full text Add to dashboard Cite

Neurological diseases are a prevalent cause of global mortality and are of growing concern when considering an ageing global population. Traditional treatments are accompanied by serious side effects including repeated treatment sessions, invasive surgeries, or infections. For example, in the case of deep brain stimulation, large, stiff, and battery powered neural probes recruit thousands of neurons with each pulse, and can invoke a vigorous immune response. This paper presents challenges in engineering and neuroscience in developing miniaturized and biointegrated alternatives, in the form of microelectrode probes. Progress in design and topology of neural implants has shifted the goal post toward highly specific recording and stimulation, targeting small groups of neurons and reducing the foreign body response with biomimetic design principles. Implantable device design recommendations, fabrication techniques, and clinical evaluation of the impact flexible, integrated probes will have on the treatment of neurological disorders are provided in this report. The choice of biocompatible material dictates fabrication techniques as novel methods reduce the complexity of manufacture. Wireless power, the final hurdle to truly implantable neural interfaces, is discussed. These aspects are the driving force behind continued research: significant breakthroughs in any one of these areas will revolutionize the treatment of neurological disorders.

show abstract

“…Electrophysiology can be studied in tissues using a calcium indicator or a voltage-sensitive dye, enabling evaluation of real-time changes in calcium flux and voltage by fluorescence microscopy. Hybrid tissues with built-in electronics further allow non-invasive monitoring of electrophysiological tissue properties in real time 91 . Moreover, external electrical stimulation can be used to interrogate tissue function.…”

Section: Modelling Sex-based Differencesmentioning

confidence: 99%

A framework for developing sex-specific engineered heart models

et al. 2021

View full text Add to dashboard Cite

The convergence of tissue engineering and patient-specific stem cell biology has enabled the engineering of in vitro tissue models that allow the study of patient-tailored treatment modalities. However, sex-related disparities in health and disease, from systemic hormonal influences to cellular-level differences, are often overlooked in stem cell biology, tissue engineering and preclinical screening. The cardiovascular system, in particular, shows considerable sex-related differences, which need to be considered in cardiac tissue engineering. In this Review, we analyse sex-related properties of the heart muscle in the context of health and disease, and discuss a framework for including sex-based differences in human cardiac tissue engineering. We highlight how sex-based features can be implemented at the cellular and tissue levels, and how sex-specific cardiac models could advance the study of cardiovascular diseases. Finally, we define design criteria for sex-specific cardiac tissue engineering and provide an outlook to future research possibilities beyond the cardiovascular system.

show abstract

Engineering Smart Hybrid Tissues with Built-In Electronics

Cited by 17 publications

References 99 publications

Real-Time Impedance Monitoring of Epithelial Cultures with Inkjet-Printed Interdigitated-Electrode Sensors

Real-Time Impedance Monitoring of Epithelial Cultures with Inkjet-Printed Interdigitated-Electrode Sensors

The Future of Neuroscience: Flexible and Wireless Implantable Neural Electronics

A framework for developing sex-specific engineered heart models

Contact Info

Product

Resources

About