volume 17, issue 8, P757-764 1995
DOI: 10.1016/0141-0229(95)91162-r
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W.J. Newsted, M. Ramjeesingh, M. Zywulko, S.J. Rothstein, E.Y. Shami

Abstract: We have demonstrated that a trypsin sensitive enzyme such as L-asparaginase can be rendered trypsin resistant by genetically fusing its gene with that of a single-chain antibody derived from a preselected monoclonal antibody capable of providing protection against trypsin. The chimeric L-asparaginase retained 75% of its original activity upon exposure to trypsin, whereas the native unprotected L-asparaginase control was totally inactivated.