Engineering microbes to sense and eradicate
            <i>Pseudomonas aeruginosa</i>
            , a human pathogen

Saeidi, Nazanin; Wong, Adison; Lo, Tat Ming; Nguyen, Hung; Ling, Hua; Leong, Susanna Su Jan; Poh, Chueh Loo

doi:10.1038/msb.2011.55

Cited by 329 publications

(308 citation statements)

References 33 publications

Supporting

Mentioning

297

Contrasting

Unclassified

Order By: Relevance

“…When cultured together, the 3OC12HSL generated by P. aeruginosa triggered E. coli to produce and release pyocin C5. The engineered E. coli was thus able to kill the pseudomonads and was effective against P. aeruginosa growing both planktonically and in biofilms (365). Though these studies were conducted only under in vitro conditions, it will be interesting to watch as developments lead toward in vivo testing.…”

Section: Qsi Success Storiesmentioning

confidence: 99%

“…As we have described above, autoinducer signals are often unique to the species that produce them and serve in some ways as a distinctive "scent" that might be traced. Using synthetic biology approaches, Saeidi et al generated an E. coli version of a bloodhound, capable of sniffing out and killing Pseudomonas aeruginosa (365). Expressed in E. coli, LasR enabled the bacterium to detect 3OC12HSL produced by P. aeruginosa.…”

Section: Qsi Success Storiesmentioning

confidence: 99%

See 1 more Smart Citation

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

676

556

View full text Add to dashboard Cite

SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

show abstract

Section: Qsi Success Storiesmentioning

confidence: 99%

Section: Qsi Success Storiesmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

676

556

View full text Add to dashboard Cite

show abstract

“…Numerous such examples have been recently described including the bioconversion of unprocessed cellulolytic feedstocks into biofuel isobutanol using fungal-bacterial communities (4) and biofuel precursor methyl halides using yeast-bacterial cocultures (5). Other emerging applications in biosensing and bioremediation against environmental toxins such as arsenic (6) and pathogens such as Pseudomonas aeruginosa and Vibrio cholerae have been demonstrated using engineered quorum-sensing Escherichia coli (7,8). These advances paint an exciting future for the development of sophisticated multispecies microbial communities to address pressing challenges and the crucial need to understand the basic principles that enables their design and engineering.…”

mentioning

confidence: 99%

Syntrophic exchange in synthetic microbial communities

Mee

Collins

Church

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

505

526

View full text Add to dashboard Cite

Metabolic crossfeeding is an important process that can broadly shape microbial communities. However, little is known about specific crossfeeding principles that drive the formation and maintenance of individuals within a mixed population. Here, we devised a series of synthetic syntrophic communities to probe the complex interactions underlying metabolic exchange of amino acids. We experimentally analyzed multimember, multidimensional communities of Escherichia coli of increasing sophistication to assess the outcomes of synergistic crossfeeding. We find that biosynthetically costly amino acids including methionine, lysine, isoleucine, arginine, and aromatics, tend to promote stronger cooperative interactions than amino acids that are cheaper to produce. Furthermore, cells that share common intermediates along branching pathways yielded more synergistic growth, but exhibited many instances of both positive and negative epistasis when these interactions scaled to higher dimensions. In more complex communities, we find certain members exhibiting keystone species-like behavior that drastically impact the community dynamics. Based on comparative genomic analysis of >6,000 sequenced bacteria from diverse environments, we present evidence suggesting that amino acid biosynthesis has been broadly optimized to reduce individual metabolic burden in favor of enhanced crossfeeding to support synergistic growth across the biosphere. These results improve our basic understanding of microbial syntrophy while also highlighting the utility and limitations of current modeling approaches to describe the dynamic complexities underlying microbial ecosystems. This work sets the foundation for future endeavors to resolve key questions in microbial ecology and evolution, and presents a platform to develop better and more robust engineered synthetic communities for industrial biotechnology.synthetic ecosystem | amino acid exchange | population modeling M icrobes are abundantly found in almost every part of the world, living in communities that are diverse in many facets. Although it is clear that cooperation and competition within microbial communities is central to their stability, maintenance, and longevity, there is limited knowledge about the general principles guiding the formation of these intricate systems. Understanding the underlying governing principles that shape a microbial community is key for microbial ecology but is also crucial for engineering synthetic microbiomes for various biotechnological applications (1-3). Numerous such examples have been recently described including the bioconversion of unprocessed cellulolytic feedstocks into biofuel isobutanol using fungal-bacterial communities (4) and biofuel precursor methyl halides using yeast-bacterial cocultures (5). Other emerging applications in biosensing and bioremediation against environmental toxins such as arsenic (6) and pathogens such as Pseudomonas aeruginosa and Vibrio cholerae have been demonstrated using engineered quorum-sensing Escherichia coli (7, 8). These ...

show abstract

“…For example, antimicrobial peptides (AMPs), such as thuricin CD [16] and pyocin S5 [17], have been utilized for narrow-spectrum targeting of pathogens, thus reducing the chance of resistance and disturbance to the gut microbiota. Another interesting strategy that has been investigated for microbiome engineering is to administer prebiotics to remodel the microbiota.…”

Section: Engineering Of Human Microbiomementioning

confidence: 99%

Microbiome engineering: Current applications and its future

Foo

Ling

Lee

2017

Biotechnology Journal

Self Cite

156

102

View full text Add to dashboard Cite

Microbiomes exist in all ecosystems and are composed of diverse microbial communities. Perturbation to microbiomes brings about undesirable phenotypes in the hosts, resulting in diseases and disorders, and disturbs the balance of the associated ecosystems. Engineering of microbiomes can be used to modify structures of the microbiota and restore ecological balance. Consequently, microbiome engineering has been employed for improving human health and agricultural productivity. The importance and current applications of microbiome engineering, particularly in humans, animals, plants and soil is reviewed. Furthermore, we explore the challenges in engineering microbiome and the future of this field, thus providing perspectives and outlook of microbiome engineering.

show abstract

Engineering microbes to sense and eradicate Pseudomonas aeruginosa , a human pathogen

Abstract: A synthetic genetic system is designed and characterized that allows Escherichia coli to sense and eradicate Pseudomonas aeruginosa, providing a novel antimicrobial strategy that could potentially be applied to fighting infectious pathogens.

Cited by 329 publications

References 33 publications

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Syntrophic exchange in synthetic microbial communities

Microbiome engineering: Current applications and its future

Contact Info

Product

Resources

About