Engineering design of a cardiac myocyte

Adams, William James; Pong, Terrence; Geisse, Nicholas A.; Sheehy, Sean P.; Diop-Frimpong, Benjamin; Parker, Kevin Kit

doi:10.1007/s10820-006-9045-6

Cited by 20 publications

(22 citation statements)

References 17 publications

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“…Cardiac ventricular myocytes were harvested from two day old neonatal Sprague-Dawley rats (Charles River Laboratories, Wilmington, MA) based on published protocols. 7 Briefly, ventricles were extracted and homogenized by washing in Hanks balanced salt solution, then incubated with 1 mg mL −1 trypsin overnight at 4 °C. Ventricular tissue was digested with 1 mg mL −1 collagenase at 37 °C for several minutes to release the myocytes into solution.…”

Section: Methodsmentioning

confidence: 99%

Ensembles of engineered cardiac tissues for physiological and pharmacological study: Heart on a chip

et al. 2011

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Traditionally, muscle physiology experiments require multiple tissue samples to obtain morphometric, electrophysiological, and contractility data. Furthermore, these experiments are commonly completed one at a time on cover slips of single cells, isotropic monolayers, or in isolated muscle strips. In all of these cases, variability of the samples hinders quantitative comparisons among experimental groups. Here, we report the design of a “heart on a chip” that exploits muscular thin film technology – biohybrid constructs of an engineered, anisotropic ventricular myocardium on an elastomeric thin film – to measure contractility, combined with a quantification of action potential propagation, and cytoskeletal architecture in multiple tissues in the same experiment. We report techniques for real-time data collection and analysis during pharmacological intervention. The chip is an efficient means of measuring structure-function relationships in constructs that replicate the hierarchical tissue architectures of laminar cardiac muscle.

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Section: Methodsmentioning

confidence: 99%

Ensembles of engineered cardiac tissues for physiological and pharmacological study: Heart on a chip

et al. 2011

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“…Neonatal rat ventricular myocytes were harvested from two day old Sprague-Dawley rats (Charles River Labarotories, Wilmington, MA) (Adams, et al, 2007). The extracted ventricles were washed in Hanks balanced salt solution and then homogenized by incubating in trypsin (1 mg/mL) for approximately twelve hours at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Muscle on a chip: In vitro contractility assays for smooth and striated muscle

Grosberg

Nesmith

Goss

et al. 2012

Journal of Pharmacological and Toxicological Methods

156

112

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Introduction To evaluate the viability of a muscle tissue, it is essential to measure the tissue’s contractile performance as well as to control its structure. Accurate contractility data can aid in development of more effective and safer drugs. This can be accomplished with a robust in vitro contractility assay applicable to various types of muscle tissue. Methods The devices developed in this work were based on the muscular thin film (MTF) technology, in which an elastic film is manufactured with a 2D engineered muscle tissue on one side. The tissue template is made by patterning extracellular matrix with microcontact printing. When muscle cells are seeded on the film, they self-organize with respect to the geometric cues in the matrix to form a tissue. Results Several assays based on the “MTF on a chip” technology are demonstrated. One such assay incorporates the contractility assay with striated muscle into a fluidic channel. Another assay platform incorporates the MTFs in a multi-well plate, which is compatible with automated data collection and analysis. Finally, we demonstrate the possibility of analyzing contractility of both striated and smooth muscle simultaneously on the same chip. Discussion In this work, we assembled an ensemble of contractility assays for striated and smooth muscle based on muscular thin films. Our results suggest an improvement over current methods and an alternative to isolated tissue preparations. Our technology is amenable to both primary harvests cells and cell lines, as well as both human and animal tissues.

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“…NRVCMs are isolated from two-day-old neonatal Sprague-Dawley rats based on published protocols [17]. All procedures are conducted in accordance with the guidelines of the Institutional Animal Care and Use Committee at University of California, Berkeley.…”

Section: Cell Isolation and Culturementioning

confidence: 99%

Energy harvesting using uniaxially aligned cardiomyocytes

Liu

Wang

et al. 2014

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS)

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This paper presents the concept of energy harvesting from uniaxially-aligned cardiomyocytes (CMs) on a flexible substrate for the first time. Experimentally, synchronously contracting neonatal rat ventricular cardiomyocytes (NRVCMs) at 0.5Hz have been found to cause the mechanical straining of a piezoelectric energy harvester to produce 87.5nA and 92.3mV of peak current and voltage, respectively.This work has been accomplished: (a) fabrication of a bio-hybrid energy harvester combining living cells, bio-compatible PDMS polymer substrate and piezoelectric PVDF films; (b) engineered living cell patterns on PDMS with uniaxially-aligned direction for enhanced mechanical actuation; and (c) up to one month of continuous synchronous contractions from NRVCMs for energy harvesting demonstration. This paper will detail the concept, design, fabrication, and experiments of the bio-hybrid energy harvester.

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Engineering design of a cardiac myocyte

Cited by 20 publications

References 17 publications

Ensembles of engineered cardiac tissues for physiological and pharmacological study: Heart on a chip

Ensembles of engineered cardiac tissues for physiological and pharmacological study: Heart on a chip

Muscle on a chip: In vitro contractility assays for smooth and striated muscle

Energy harvesting using uniaxially aligned cardiomyocytes

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