Engineering crop plants: getting a handle on phosphate

Brinch-Pedersen, Henrik; Sørensen, Lisbeth Dahl; Holm, Preben Bach

doi:10.1016/s1360-1385(01)02222-1

Cited by 252 publications

(168 citation statements)

References 55 publications

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“…3 H-I(1,3,4,6)P 4 was synthesized as described (11). 3 Ci [ 3 H] myo-inositol for 2 days at 20°C, constant light. IPs were harvested from seeds at the late bent cotyledon stage of development (24) that were dissected from whole siliques exactly as described for seedlings.…”

Section: Methodsmentioning

confidence: 99%

“…In developing countries, high-phytate grain-based diets are feared to exacerbate iron and zinc malnutrition. In developed countries, where livestock is fed primarily grain-based feed, the excreted phytate contributes to environmental phosphorus pollution by washing into surface waters, where it accelerates eutrophication (3). Collectively, these problems have provided a strong impetus to develop seed that is reduced in phytate and its inositol polyphosphate (IP) precursors.…”

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confidence: 99%

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Generation of phytate-free seeds in Arabidopsis through disruption of inositol polyphosphate kinases

Stevenson-Paulik

Bastidas

Chiou

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

276

359

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Phytate (inositol hexakisphosphate, IP6) is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Here, we report a requirement for inositol polyphosphate kinases, AtIPK1 and AtIPK2␤, for the later steps of phytate synthesis in Arabidopsis thaliana. Coincident disruption of these kinases nearly ablates seed phytate without accumulation of phytate precursors, increases seed-free phosphate by 10-fold, and has normal seed yield. Additionally, we find a requirement for inositol tetrakisphosphate (IP 4)͞inositol pentakisphosphate (IP 5) 2-kinase activity in phosphate sensing and root hair elongation. Our results define a commercially viable strategy for the genetic engineering of phytate-free grain and provide insights into the role of inositol polyphosphate kinases in phosphate signaling biology.genetically engineered crops ͉ low phytate ͉ signal transduction ͉ phosphate sensing

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Generation of phytate-free seeds in Arabidopsis through disruption of inositol polyphosphate kinases

Stevenson-Paulik

Bastidas

Chiou

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

276

359

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“…Availability of phosphorus in the soil strongly determines the plant growth, metabolism, development, reproduction and crop yield (Marschner 1995;Brinch-Pedersen et al 2002;Hermans et al 2006). Phosphorus is well known as an essential nutrient important in many processes like photosynthesis, respiration, regulation of enzymes activity and gene expression or signal transduction (Raghothama 1999;Rychter and Rao 2005;Calderon-Vazquez et al 2008;Nilsson et al 2010;Yao et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Acid phosphatases and growth of barley (Hordeum vulgare L.) cultivars under diverse phosphorus nutrition

2011

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The morphological and physiological responses of barley to moderate Pi deficiency and the ability of barley to grow on phytate were investigated. Barley cultivars (Hordeum vulgare L., Promyk, Skald and Stratus) were grown for 1-3 weeks on different nutrient media with contrasting phosphorus source: KH 2 PO 4 (control), phytic acid (PA) and without phosphate (-P). The growth on -P medium strongly decreased Pi concentration in the tissues; culture on PA medium generally had no effect on Pi level.

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“…Hereby, phosphate, inositol phosphates, and inositol are provided for a range of cellular activities (Brinch-Pedersen et al, 2002). A number of enzymes with phytase activity are known from plants, animals, and microorganisms (Dvoráková, 1998).…”

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confidence: 99%

Cloning and Characterization of Purple Acid Phosphatase Phytases from Wheat, Barley, Maize, and Rice

et al. 2011

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Barley (Hordeum vulgare) and wheat (Triticum aestivum) possess significant phytase activity in the mature grains. Maize (Zea mays) and rice (Oryza sativa) possess little or virtually no preformed phytase activity in the mature grain and depend fully on de novo synthesis during germination. Here, it is demonstrated that wheat, barley, maize, and rice all possess purple acid phosphatase (PAP) genes that, expressed in Pichia pastoris, give fully functional phytases (PAPhys) with very similar enzyme kinetics. Preformed wheat PAPhy was localized to the protein crystalloid of the aleurone vacuole. Phylogenetic analyses indicated that PAPhys possess four conserved domains unique to the PAPhys. In barley and wheat, the PAPhy genes can be grouped as PAPhy_a or PAPhy_b isogenes (barley, HvPAPhy_a, HvPAPhy_b1, and HvPAPhy_b2; wheat, TaPAPhy_a1, TaPAPhy_a2, TaPAPhy_b1, and TaPAPhy_b2). In rice and maize, only the b type (OsPAPhy_b and ZmPAPhy_b, respectively) were identified. HvPAPhy_a and HvPAPhy_b1/b2 share 86% and TaPAPhya1/a2 and TaPAPhyb1/b2 share up to 90% (TaPAPhy_a2 and TaPAPhy_b2) identical amino acid sequences. despite of this, PAPhy_a and PAPhy_b isogenes are differentially expressed during grain development and germination. In wheat, it was demonstrated that a and b isogene expression is driven by different promoters (approximately 31% identity). TaPAPhy_a/b promoter reporter gene expression in transgenic grains and peptide mapping of TaPAPhy purified from wheat bran and germinating grains confirmed that the PAPhy_a isogene set present in wheat/barley but not in rice/maize is the origin of high phytase activity in mature grains.

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Engineering crop plants: getting a handle on phosphate

Cited by 252 publications

References 55 publications

Generation of phytate-free seeds in Arabidopsis through disruption of inositol polyphosphate kinases

Generation of phytate-free seeds in Arabidopsis through disruption of inositol polyphosphate kinases

Acid phosphatases and growth of barley (Hordeum vulgare L.) cultivars under diverse phosphorus nutrition

Cloning and Characterization of Purple Acid Phosphatase Phytases from Wheat, Barley, Maize, and Rice

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