Engineering and microbiological aspects of BTEX removal in bioreactors under sulfate-reducing conditions

Firmino, Paulo Igor Milen; Farias, Raquel Studart de; Buarque, Patrícia Marques Carneiro; Costa, Mayara Carantino; Rodriguéz, Elisa; Lopes, Alexandre Colzi; Santos, André Bezerra dos

doi:10.1016/j.cej.2014.08.111

Cited by 30 publications

(27 citation statements)

References 35 publications

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“…In the marine sediments, the degradation of crude oil was limited by the availability of N and P. But addition of simple empirical estimates of the nutrient proportions may not be sufficient for successful remediation (Singh et al 2015). Labile organic enhancer substrates used like acetate (Stasik et al 2015) and ethanol (Firmino et al 2015) exhibited negative results possibly due to product inhibition in the degradative pathway. (Mukherjee and Bordoloi 2012) had reported the essential presence of minimum amount of phosphate and nitrate sources and the fact that S. silvestris AM1 could produce bioemulsifier only in ZM medium,1/10th ZM medium was chosen.…”

Section: Microcosm Studiesmentioning

confidence: 95%

Microcosm-based interaction studies between members of two ecophysiological groups of bioemulsifier producer and a hydrocarbon degrader from the Indian intertidal zone

Markande

Nerurkar

2016

Environ Sci Pollut Res

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Isolates were obtained from intertidal zone site samples from all five western and one eastern coastal states of India and were screened. These ecophysiological groups of aerobic, mesophilic, heterotrophic, sporulating, and bioemulsifier-producing bacteria were from Planococcaceae and Bacillaceae. This is the first report of bioemulsifier production by Sporosarcina spp., Lysinibacillus spp., B. thuringiensis, and B. flexus. In this group, Solibacillus silvestris AM1 was found to produce the highest emulsification activity (62.5 %EI) and the sample that yielded it was used to isolate the ecophysiological group of non-bioemulsifier-producing, hydrocarbon-degrading bacteria (belonging to Chromatiales and Bacillales). These yielded hitherto unreported degrader, Rheinheimera sp. CO6 which was selected for the interaction studies (in a microcosm) with bioemulsifier-producing S. silvestris AM1. The gas chromatographic study of these microcosm experiments revealed increased degradation of benzene, toluene, and xylene (BTX) and the growth of Rheinheimera sp. CO6 in the presence of bioemulsifier produced by S. silvestris AM1. Enhancement of the growth of S. silvestris AM1 in the presence of Rheinheimera sp. CO6 was observed possibly due to reduced toxicity of BTX suggesting mutualistic association between the two. This study elucidates the presence and interaction between enhancers and degraders in a hydrocarbon-contaminated intertidal zone and contributes to the knowledge during application of the two in remediation processes.

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Section: Microcosm Studiesmentioning

confidence: 95%

Microcosm-based interaction studies between members of two ecophysiological groups of bioemulsifier producer and a hydrocarbon degrader from the Indian intertidal zone

Markande

Nerurkar

2016

Environ Sci Pollut Res

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“…The PCR mixture (50 lL) contained 10 lL of reaction buffer (5X), 5 lL of MgCl 2 (25 mM), 0.25 lL of Taq polymerase (5 u Á lL À1 ) (Promega, USA), 1.0 or 1.5 lL of deoxynucleotide triphosphates (10 mM), l lL of the extracted DNA, 1.0 or 1.5 lL of PCR primers (10 lM) and Milli-Q water up to a final volume of 50 lL. The PCR thermocycling program used was previously described by Firmino et al [21].…”

Section: Microbial Community Analysesmentioning

confidence: 99%

“…DGGE was performed in a D-Code Universal Mutation Detection System (Bio Rad Laboratories, USA) using polyacrylamide gels with a urea/formamide denaturing gradient of 42-67% and 30-60% for bacterial and archaeal communities, respectively and superimposed with a porous gradient of acrylamide/bisacrylamide (6-10%). The electrophoresis conditions and gel staining were previously described by Firmino et al [21].…”

Section: Microbial Community Analysesmentioning

confidence: 99%

“…Biogas characterization was carried out, in terms of air (O 2 + N 2 ), CO 2 and CH 4 , by gas chromatography with thermal conductivity detection (GC-TCD) (GC-17A, Shimadzu Corporation, Japan) as described by Firmino et al [21].…”

Section: Chemical and Chromatographic Analysesmentioning

confidence: 99%

“…Genomic DNA was extracted according to Firmino et al [21]. The universal bacterial primers 968-F-GC and 1401-R, and the archaeal primers A109(T)-F and 515-GC-R (IDT, USA) were used in the polymerase chain reaction (PCR).…”

Section: Microbial Community Analysesmentioning

confidence: 99%

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Understanding the anaerobic BTEX removal in continuous-flow bioreactors for ex situ bioremediation purposes

Firmino

Farias

Barros

et al. 2015

Chemical Engineering Journal

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Impact of Microaeration and the Redox Mediator Anthraquinone‐2,6‐Disulfonate on Azo Dye Reduction and By‐Products Degradation

Barros

Silva

Firmino

et al. 2018

CLEAN Soil Air Water

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This work assessed the impact of microaeration and the redox mediator anthraquinone-2,6-disulfonate (AQDS) on both azo dye reduction and by-products degradation (aromatic amines) in a mesophilic reactor. The experiment was carried out in an up-flow anaerobic sludge blanket (UASB) reactor with 3.2 L of working volume and hydraulic retention time (HRT) of 24 h. The synthetic effluent contained 50 mg L À1 of the dye Reactive Red 2 (RR2). The reactor was initially operated under anaerobic conditions and, subsequently, was microaerated with atmospheric air (1 mL min À1 ) and then supplemented with the redox mediator (AQDS) at a concentration of 25 μM. The microaeration effect on dye reduction and by-products formed was not evident. However, AQDS considerably enhanced RR2 reduction, and its regeneration was not affected by the presence of oxygen. It seems that the cleavage of the double bond between nitrogen groups is a much easier way to start the attack of the azo dye, and it is independent of the presence of oxygen. Thus, the introduction of hydroxyl groups into the aromatic ring was not playing a role in the color removal process. In terms of ecological parameters, the ecological niches were maintained, and the distribution of species increased, despite the majority being generalists, i.e., not specialized in dye degradation. Furthermore, anaerobic species are dominant over facultative species, which explains why there was no population diversity recovery after the microaeration started.

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Engineering and microbiological aspects of BTEX removal in bioreactors under sulfate-reducing conditions

Cited by 30 publications

References 35 publications

Microcosm-based interaction studies between members of two ecophysiological groups of bioemulsifier producer and a hydrocarbon degrader from the Indian intertidal zone

Microcosm-based interaction studies between members of two ecophysiological groups of bioemulsifier producer and a hydrocarbon degrader from the Indian intertidal zone

Understanding the anaerobic BTEX removal in continuous-flow bioreactors for ex situ bioremediation purposes

Impact of Microaeration and the Redox Mediator Anthraquinone‐2,6‐Disulfonate on Azo Dye Reduction and By‐Products Degradation

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