Engineered Skin Tissue Equivalents for Product Evaluation and Therapeutic Applications

Suhail, Sana; Sardashti, Naseem; Jaiswal, Devina; Rudraiah, Swetha; Misra, Manoj; Kumbar, Sangamesh G.

doi:10.1002/biot.201900022

Cited by 51 publications

(19 citation statements)

References 114 publications

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“…that could be in direct contact with human skin, raises additional questions of potential toxicity from dermal exposure. Thus, bioengineered skin models are also important investigation to determine the extent of penetration and of nanomaterials, but also several environmental pollutants and irritants [32][33][34][35]. Unique characteristics of human skin, such as pigmentation, dermal evolution, presence of adipose tissue, and skin appendage distribution, render skin behavior comparison between other mammalian species inaccurate.…”

Section: Introductionmentioning

confidence: 99%

“…Unique characteristics of human skin, such as pigmentation, dermal evolution, presence of adipose tissue, and skin appendage distribution, render skin behavior comparison between other mammalian species inaccurate. Since the structure and organization vary between mammalians, the penetration and absorption into the human skin cannot be completely predicted by studying them on animal models [35].…”

Section: Introductionmentioning

confidence: 99%

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Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

et al. 2020

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Limitations in wound management have prompted scientists to introduce bioprinting techniques for creating constructs that can address clinical problems. The bioprinting approach is renowned for its ability to spatially control the three-dimensional (3D) placement of cells, molecules, and biomaterials. These features provide new possibilities to enhance homology to native skin and improve functional outcomes. However, for the clinical value, the development of hydrogel bioink with refined printability and bioactive properties is needed. In this study, we combined the outstanding viscoelastic behavior of nanofibrillated cellulose (NFC) with the fast cross-linking ability of alginate (ALG), carboxymethyl cellulose (CMC), and encapsulated human-derived skin fibroblasts (hSF) to create a bioink for the 3D bioprinting of a dermis layer. The shear thinning behavior of hSF-laden bioink enables construction of 3D scaffolds with high cell density and homogeneous cell distribution. The obtained results demonstrated that hSF-laden bioink supports cellular activity of hSF (up to 29 days) while offering proper printability in a biologically relevant 3D environment, making it a promising tool for skin tissue engineering and drug testing applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

et al. 2020

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“…Skin is to act as physical barriers that protect the tissues from chemical, physical, and microbial agents. Early in vitro skin models as reconstructed human epidermis (RHE) models consisted of two dimensional and later developed three-dimensional cell cultures [ 85 ]. RHE models can be used to study permeability and absorption; however, they do not include endothelial cells and evaluate irritation based on cell viability (CV) [ 82 ].…”

Section: Utilization Of Skin-on-a-chip Systemsmentioning

confidence: 99%

Development of Skin-On-A-Chip Platforms for Different Utilizations: Factors to Be Considered

et al. 2021

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There is increasing interest in miniaturized technologies in diagnostics, therapeutic testing, and biomedicinal fundamental research. The same is true for the dermal studies in topical drug development, dermatological disease pathology testing, and cosmetic science. This review aims to collect the recent scientific literature and knowledge about the application of skin-on-a-chip technology in drug diffusion studies, in pharmacological and toxicological experiments, in wound healing, and in fields of cosmetic science (ageing or repair). The basic mathematical models are also presented in the article to predict physical phenomena, such as fluid movement, drug diffusion, and heat transfer taking place across the dermal layers in the chip using Computational Fluid Dynamics techniques. Soon, it can be envisioned that animal studies might be at least in part replaced with skin-on-a-chip technology leading to more reliable results close to study on humans. The new technology is a cost-effective alternative to traditional methods used in research institutes, university labs, and industry. With this article, the authors would like to call attention to a new investigational family of platforms to refresh the researchers’ theranostics and preclinical, experimental toolbox.

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“…As a result, skin tissue grafts are commercially available reducing the need for autologous grafts from other sites and the associated morbidity. 138,139 However, limitations will always exist necessitating continual improvement. Acellular membranes, such as human acellular amniotic membranes separated from consenting mother placentas, have been used in clinical trials.…”

Section: Mucosa Periodontal and Skin Tissue Engineeringmentioning

confidence: 99%

Recent update on craniofacial tissue engineering

Emara

Shah²

2021

J Tissue Eng

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The craniofacial region consists of several different tissue types. These tissues are quite commonly affected by traumatic/pathologic tissue loss which has so far been traditionally treated by grafting procedures. With the complications and drawbacks of grafting procedures, the emerging field of regenerative medicine has proved potential. Tissue engineering advancements and the application in the craniofacial region is quickly gaining momentum although most research is still at early in vitro/in vivo stages. We aim to provide an overview on where research stands now in tissue engineering of craniofacial tissue; namely bone, cartilage muscle, skin, periodontal ligament, and mucosa. Abstracts and full-text English articles discussing techniques used for tissue engineering/regeneration of these tissue types were summarized in this article. The future perspectives and how current technological advancements and different material applications are enhancing tissue engineering procedures are also highlighted. Clinically, patients with craniofacial defects need hybrid reconstruction techniques to overcome the complexity of these defects. Cost-effectiveness and cost-efficiency are also required in such defects. The results of the studies covered in this review confirm the potential of craniofacial tissue engineering strategies as an alternative to avoid the problems of currently employed techniques. Furthermore, 3D printing advances may allow for fabrication of patient-specific tissue engineered constructs which should improve post-operative esthetic results of reconstruction. There are on the other hand still many challenges that clearly require further research in order to catch up with engineering of other parts of the human body.

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Engineered Skin Tissue Equivalents for Product Evaluation and Therapeutic Applications

Cited by 51 publications

References 114 publications

Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

Development of Skin-On-A-Chip Platforms for Different Utilizations: Factors to Be Considered

Recent update on craniofacial tissue engineering

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