Engineered Resistance to Tomato Spotted Wilt Virus, a Negative–Strand RNA Virus

Gielen, J.; Haan, Peter; Kool, A. J.; Peters, D.; Grinsven, Mart Q. J. M. van; Goldbach, Rob

doi:10.1038/nbt1291-1363

Cited by 98 publications

(39 citation statements)

References 39 publications

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“…Like in the case of CMV, pathogen-derived resistance genes have been described for both viruses (Gielen et al ., 1991 ;MacKenzie & Ellis, 1992 ;van der Vlugt et al ., 1992), and in case of TSWV have already been applied in tomato (Ultzen et al ., 1995) . Since breeding for disease resistance is generally regarded as the best approach for sustainable crop protection, the combination of multiple pathogen-derived resistance genes within single tomato cultivars to combat plant viral diseases, represents a promising strategy towards the insecticide free culture of tomato .…”

Section: Discussionmentioning

confidence: 99%

Coat protein-mediated protection to cucumber mosaic virus infections in cultivated tomato

Gielen¹,

Ultzen²,

Bontems³

et al. 1996

Euphytica

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Cucumber mosaic virus (CMV) infections rank among the most devastating diseases in the commercial culture of tomato (Lycopersicon esculentum Mill .), for which suitable sources of natural resistance are not available . The concept of pathogen-derived resistance, however, offers an alternate approach to combat plant viral diseases by transformation of crops with nucleotide sequences derived from the viral genome . This report demonstrates the successful application of such a pathogen-derived resistance gene comprising the CMV coat protein (CP) gene, to generate protection to CMV infections in cultivated tomato . Transformation of an inbred tomato line with the CMV CP gene isolated from a subgroup I strain, engendered high levels of protection to various CMV strains, including a virulent strain causing lethal necrosis and a typical subgroup II strain . Moreover, when challenged by natural infection through aphid vectors in open field, levels of protection were largely maintained in hemizygous hybrids . In all, these results demonstrate that synthetic resistance genes based on the CMV CP gene make excellent sources of broad spectrum resistance to CMV infections for introgression into tomato breeding programs .

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Section: Discussionmentioning

confidence: 99%

Coat protein-mediated protection to cucumber mosaic virus infections in cultivated tomato

Gielen¹,

Ultzen²,

Bontems³

et al. 1996

Euphytica

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“…The N gene has been extensively used for TSWV protection (Gielen et al 1991, MacKenzie and Ellis 1992, de Haan et al 1992. The NSm gene was also found to be effective for conferring RNA-mediated virus resistance (Prins et al 1996) to plants.…”

Section: Discussionmentioning

confidence: 99%

“…Resistance to TSWV was first reported by Gielen et al (1991) and MacKenzie and Ellis (1992) in plants transformed with the N gene of TSWV. Since there was no correlation between the amount of N and the level of resistance, de Haan et al (1992) suggested that the resistance was Communicated by M. OmuraRNA-mediated.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Virus Resistance Conferred by the NSs Gene Sequences from <i>Tomato spotted wilt virus</i> in Transgenic Plants

2005

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Transgenic Nitcotiana benthamiana expressing the sense NSs gene sequence from Tomato spotted wilt virus (TSWV) was further transformed with the sense or antisense NSs gene sequence. Three sense and 10 antisense lines with two transformations (hereafter referred to as "double-transformed lines") that were expected to contain only the sense and both sense and antisense NSs gene sequences, respectively, were examined for homology-dependent virus resistance using the unrelated virus, Potato virus X containing the NSs gene sequence. As a result, it was shown that two antisense double-transformed lines contained plants that were symptom-free. Furthermore, both lines accumulated a small-interfering RNA. These results suggest that RNA silencing was operating in both lines. However, the lines exhibited little or no resistance to TSWV.

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“…Since then, many successful examples of PDR have been reported using numerous viral genes and sequences (Beachy 1997). Virus resistance to TSWV was first reported by Gielen et al (1991) and MacKenzie and Ellis (1992) in plants transformed with the N gene of TSWV. They showed that the N gene of TSWV is effective as a source of PDR.…”

Section: Short Communicationmentioning

confidence: 98%

Analysis of gene sequences for the nucleocapsid protein from Tomato spotted wilt virus for promoting RNA-mediated cross-protection using the Potato virus X vector system

Sonoda

Tsumuki

2004

J Gen Plant Pathol

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Virus interactions between Tomato spotted wilt virus (TSWV) andPotato virus X (PVX) containing the nucleocapsid protein (N) gene sequences were examined to evaluate the capacity of the N gene sequences from TSWV to promote RNA-mediated cross-protection. Plants simultaneously inoculated with TSWV and PVX containing the 3Ј 96 bp of the N gene were highly resistant to TSWV infection, whereas no such resistance was observed in plants inoculated with TSWV and PVX containing the 5Ј 96 bp. These results suggest that the 3Ј portion of the N gene has a higher capacity for promoting RNA-mediated crossprotection of TSWV.

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Engineered Resistance to Tomato Spotted Wilt Virus, a Negative–Strand RNA Virus

Cited by 98 publications

References 39 publications

Coat protein-mediated protection to cucumber mosaic virus infections in cultivated tomato

Coat protein-mediated protection to cucumber mosaic virus infections in cultivated tomato

Evaluation of Virus Resistance Conferred by the NSs Gene Sequences from <i>Tomato spotted wilt virus</i> in Transgenic Plants

Analysis of gene sequences for the nucleocapsid protein from Tomato spotted wilt virus for promoting RNA-mediated cross-protection using the Potato virus X vector system

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