Engineered high-strength biohydrogel as a multifunctional platform to deliver nucleic acid for ameliorating intervertebral disc degeneration

Chen, Tao; Qian, Qiuping; Makvandi, Pooyan; Zare, Ehsan Nazarzadeh; Chen, Qizhu; Chen, Linjie; Zhang, Zhiguang; Zhang, Hao; Zhou, Wenxian; Wang, Hui; Wang, Xiangyang; Chen, Yu; Zhou, Yunlong; Wu, Ai-Min

doi:10.1016/j.bioactmat.2023.01.010

Cited by 13 publications

(8 citation statements)

References 58 publications

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“…This structure plays a vital role in water retention in the NP tissue and imparts unique mechanical properties to the IVD. SOX-9, a protein that promotes cartilage development, is an essential component involved in the repair of ECM, thus delaying intervertebral disc degeneration IVDD . Currently, existing research has demonstrated that an excess of ROS can induce the overproduction of hydrolases in NPCs by modulating the epigenetics of NPCs and activating the cGAS-Sting axis. , Under the interference of ROS, NPCs produce a large number of matrix metalloproteinases (MMPs) and proteoglycans, such as ADAMTS.…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial-Targeted Metal-Phenolic Nanoparticles to Attenuate Intervertebral Disc Degeneration: Alleviating Oxidative Stress and Mitochondrial Dysfunction

Chen,

Qian,

et al. 2024

ACS Nano

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As intervertebral disc degeneration (IVDD) proceeds, the dysfunctional mitochondria disrupt the viability of nucleus pulposus cells, initiating the degradation of the extracellular matrix. To date, there is a lack of effective therapies targeting the mitochondria of nucleus pulposus cells. Here, we synthesized polygallic acid-manganese (PGA-Mn) nanoparticles via self-assembly polymerization of gallic acid in an aqueous medium and introduced a mitochondrial targeting peptide (TP04) onto the nanoparticles using a Schiff base linkage, resulting in PGA-Mn-TP04 nanoparticles. With a size smaller than 50 nm, PGA-Mn-TP04 possesses pH-buffering capacity, avoiding lysosomal confinement and selectively accumulating within mitochondria through electrostatic interactions. The rapid electron exchange between manganese ions and gallic acid enhances the redox capability of PGA-Mn-TP04, effectively reducing mitochondrial damage caused by mitochondrial reactive oxygen species. Moreover, PGA-Mn-TP04 restores mitochondrial function by facilitating the fusion of mitochondria and minimizing their fission, thereby sustaining the vitality of nucleus pulposus cells. In the rat IVDD model, PGA-Mn-TP04 maintained intervertebral disc height and nucleus pulposus tissue hydration. It offers a nonoperative treatment approach for IVDD and other skeletal muscle diseases resulting from mitochondrial dysfunction, presenting an alternative to traditional surgical interventions.

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Section: Resultsmentioning

confidence: 99%

Mitochondrial-Targeted Metal-Phenolic Nanoparticles to Attenuate Intervertebral Disc Degeneration: Alleviating Oxidative Stress and Mitochondrial Dysfunction

Chen,

Qian,

et al. 2024

ACS Nano

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“…In addition, in the use of antagomir, Chen et al (2023b) found that antagomirs based on miR-204-3p can inhibit the apoptosis of NPCs and designed a hydrogel for delivery, showing good IVD recovery, water retention, and structural maintenance. Wang et al (2022b) ; Wang et al (2023a) ; Wang et al (2023b) were concerned about the role of miRNA-21 in IDD in NPC abnormal proliferation and ECM degradation, so they chose antagomir-21 to selectively inhibit miRNA-21, achieving the effect of regulating ECM metabolic balance through the inhibition of the MAPK/ERK signaling pathway, delaying the progression of IDD and achieving IDD repair.…”

Section: Therapeutic Factorsmentioning

confidence: 99%

Therapeutic factors and biomaterial-based delivery tools for degenerative intervertebral disc repair

Song,

Guo,

et al. 2024

Front. Cell Dev. Biol.

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Intervertebral disc degeneration (IDD) is the main cause of low back pain (LBP), which significantly impacts global wellbeing and contributes to global productivity declines. Conventional treatment approaches, encompassing conservative and surgical interventions, merely serve to postpone the advancement of IDD without offering a fundamental reversal. Consequently, there is an urgent demand for an effective approach to prevent the progression of IDD. Recent investigations focusing on the treatment of IDD utilizing diverse bioactive substances integrated within various biomaterials have exhibited promising outcomes. Various bioactive substances, encompassing conventional small molecule drugs, small molecule nucleic acids, and cell therapies, exhibit distinct capacities for repairing IDD. Additionally, various biological material delivery systems, such as nano micelles, microspheres, and hydrogels, possess diverse biological and release characteristics. Consequently, these diverse materials and drugs hold promise for advancing the treatment of IDD. This article aims to provide a concise overview of the IDD process and investigate the research advancements in biomaterials and bioactive substances for IDD treatment, delving into their mechanisms.

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“…25 Carboxylates, 26,27 on the other hand, may act as metal ion chelators or disrupt bacterial cell walls by interfering with their structural integrity. 28,29 However, these studies often rely on other metal ions to confer carboxylated polymers’ antimicrobial properties, leading to suboptimal antimicrobial performance. For instance, the combination of carboxyl polymers with zinc ions demonstrates only marginal improvements in their individual effectiveness.…”

Section: Introductionmentioning

confidence: 99%

Enhancing antibacterial properties by regulating valence configurations of copper: a focus on Cu-carboxyl chelates

Qian,

Chen,

Qin

et al. 2024

J. Mater. Chem. B

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Engineered high-strength biohydrogel as a multifunctional platform to deliver nucleic acid for ameliorating intervertebral disc degeneration

Cited by 13 publications

References 58 publications

Mitochondrial-Targeted Metal-Phenolic Nanoparticles to Attenuate Intervertebral Disc Degeneration: Alleviating Oxidative Stress and Mitochondrial Dysfunction

Mitochondrial-Targeted Metal-Phenolic Nanoparticles to Attenuate Intervertebral Disc Degeneration: Alleviating Oxidative Stress and Mitochondrial Dysfunction

Therapeutic factors and biomaterial-based delivery tools for degenerative intervertebral disc repair

Enhancing antibacterial properties by regulating valence configurations of copper: a focus on Cu-carboxyl chelates

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