Engineered CpG‐Antigen Conjugates Protected Gold Nanoclusters as Smart Self‐Vaccines for Enhanced Immune Response and Cell Imaging

Abstract

A facile one-pot synthesis of fl uorescent AuNCs by using ovalbumin-CpG oligodeoxynucleotides (ODNs) conjugates as the templates, which can elicit specifi c immunological responses, is demonstrated. Through dual-delivery of protein antigen and CpG ODNs, the as-prepared AuNCs can act as smart self-vaccines to assist in generation of high immunostimulatory activity while simultaneously act as an imaging agent. The AuNCs-based vaccines are not thought to have not been reported so far, making this platform the fi rst example of the usage of AuNCs as effective vaccines. Furthermore, compared with the antigen, the immunostimulatory activity of the as-prepared AuNCs can not only be retained, but also can be effectively enhanced. These fi ndings suggest the AuNCs-based vaccines may be utilized as safe and effi cient immunostimulatory agents that are able to prevent and/or treat a variety of ailments.

Design of a novel vaccine nanotechnology-based delivery system comprising CpGODN-protein conjugate anchored to liposomes

“…Overall, these results proved that the chemical linkage of TLR9 agonist CpGODN with antigenic protein confers a faster and stronger adjuvant effect towards protein antigen compared to co-administration of antigen and CpGODN, as previously reported [14-16, 45, 46, 60]. The direct linkage of protein antigen and CpG, ensures antigen and CpGODN uptake by the same APCs, resulting in a higher response toward the antigen, with a lower adjuvant dose [61][62][63].…”

Growth of Au Nanoparticles on 2D Metalloporphyrinic Metal‐Organic Framework Nanosheets Used as Biomimetic Catalysts for Cascade Reactions

“…The SAED pattern further confirms the high crystallinity of Au NPs (Figure 1h). [31] All the aforementioned results confirm the successful preparation of Au NPs/Cu-TCPP(Fe) hybrid nanosheets. [31] All the aforementioned results confirm the successful preparation of Au NPs/Cu-TCPP(Fe) hybrid nanosheets.…”

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

“…S13), which evaluated the mitochondrial activity of viable cells [90, 91]. As expected, all breast cells maintained above 90% cell viability after incubation with AuNCs at tested concentrations for 24 h, demonstrating low cytotoxicity.…”