Enginar Yaprağı Sulu Ekstraktı Kullanılarak Çinko Oksit Nanopartiküllerinin Yeşil Sentezi, Karakterizasyonu, Anti-Bakteriyel ve Sitotoksik Etkileri

Abstract

ÖZAmaç: Bu çalışmanın amacı, yeşil kimya yöntemiyle çinko oksit nanopartiküllerini (ZnONPs) sentezlemek ve bu nanopartiküllerin anti-bakteriyel ve anti-kanser etkilerini incelemektir. Gereç ve Yöntemler: Çinko iyonları ve sulu enginar yaprağı (Cynara scolymus) ekstraktı kullanılarak ZnONPs yeşil kimya yöntemiyle sentezlendi. ZnONPs oluşumunun doğrulanması ve karakterizasyonu için morötesi-görünür bölge spektroskopisi (UV-Vis), Fourier dönüşümü kızılötesi spektroskopisi (FTIR), taramalı elektron mikroskobu (SEM), zetasizer ve Enerji dağınım X-ışını spektroskopisi (EDX) analizleri kullanıldı. ZnONPs'nin 4 farklı bakteri türü (E. coli, S. aureus, P. aeruginosa ve E. faecalis) üzerindeki antibakteriyel aktiviteleri, minimal inhibe edici konsantrasyon (MİK) ve kuyucuk difüzyon yöntemiyle ölçüldü. ZnONPs'nin HT-29 insan kolon kanseri hücreleri üzerindeki sitotoksik etkileri konsantrasyon ve zamana bağlı olarak olarak belirlendi. Bulgular: UV-Vis spektrumunda ZnO'ya spesifik olan 320-335 nm aralığında absorbans artışı gözlemlendi. FTIR spektrumunda 426 cm -1 ve 540 cm -1 'de ZnO'ya ait gerilme titreşimleri belirlendi. SEM analizinde partikül boyutu 276-309 nm ölçüldü. ZnONPs'nin zeta-sizer analizlerinde partikül büyüklüğü 137,8 nm ve partikül yükü -6,34 meV olarak bulundu. Antibakteriyel aktivite ölçümlerinde, sentezlenen nanopartiküllerin E. coli ve S. aureus'ta bakteriyel aktivite inhibisyonu sağladığı tespit edildi. ZnONPs HT-29 kolon kanseri hücreleri üzerinde 10 µg/mL'den daha yüksek konsantrasyonlarda sitotoksik etki gösterdi. Sonuç: ZnONPs'nin düşük maliyetle hazırlanabileceği ve klinik tedavilerde yeni ilaç formülasyonları için taşıyıcı sistem olarak kullanılma potansiyeline sahip olduğu bu çalışma ile gösterilmiştir. ABSTRACTAim: The aim of this study is to synthesize zinc oxide nanoparticles (ZnONPs) by green chemistry method and investigate anti-bacterial and anticancer effects of these nanoparticles. Material and Methods: ZnONPs were synthesized by the green chemistry method using zinc ions and aqueous artichoke leaf (Cynara scolymus) extract. Ultraviolet-visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), zetasizer and energy dispersive X-ray spectroscopy (EDX) were used to confirm the formation and characterization of ZnONPs. Antibacterial activities of ZnONPs on four different bacteria species (E. coli, S. aureus, P. aeruginosa and E. faecalis) were measured by minimal inhibitory concentration (MIC) and agar well diffusion method. Cytotoxic effects of ZnONPs on HT-29 human colon cancer cells were determined as concentration and time dependent. Results: In the UV-Vis spectrum, absorbance increase was observed in 320-335 nm range which is specific to ZnO. In the FTIR spectrum, stretching vibrations of ZnO were determined in 426 cm -1 and 540 cm -1 . The particle size was 276-309 nm in SEM analysis. In the zeta-sizer measurements of ZnONPs, the particle size was 137.8 nm and the particle charge was -6.34 meV. In the antibacterial activity measu...

The Synthesis of Novel Aminoacid Based Amides and Evaluation of Anticancer Effects on Hela Cervical Cancer Cells

“…The cells were cultured in T75 flasks containing DMEM supplemented with 10% FBS and 1% penicillin-streptomycin antibiotics suspension and were maintained in a humidified atmosphere, containing 5% CO 2 at 37 °C. The grown cells were removed with the help of 0.05% Trypsin-EDTA (Sigma T-4049) and passaged (Çıkla et al, 2013;Erdogan et al, 2019). Morphological changes of the cells were checked under an invert microscope (Olympus CK40).…”

Biosynthesis, Characterization and Determination of Sun Protection Factor (Spf) of Iron Nanoparticles With Bee Bread

“…In this respect, nanoparticles constitute the basic building blocks of nanotechnology [4]. Although the usage area of nanoparticles is quite wide, a few of these areas are as follows; the food, health, aerospace and pharmaceutical and cosmetic industries [5].…”

“…There are 3 different synthesis methods for the synthesis of nanoparticles; physical, chemical and biological. The negative effects of physical and chemical synthesis methods are replaced left to the biological synthesis method, which is a less costly and environmentally friendly method [5]. With biological green synthesis, it is possible to synthesize nanoparticles in an environmentally friendly and easy way without the need for toxic chemicals, high temperature, high pressure and high energy [6].…”

Green Synthesis and Characterization of Anticancer Effected Silver Nanoparticles with Silverberry (Elaeagnus angustifolia) Fruit Aqueous Extract

“…There are three basic approaches in the synthesis of silver nanoparticles: physical, chemical, and biological. Since physical and chemical methods have some disadvantages such as expensive equipment, waste of time and the use of toxic materials, biological methods are more preferred in the synthesis of silver nanoparticles (Erdoğan et al, 2019;Zhang et al, 2021). Biocomponents found in biological materials play a role in synthesis reactions as reducing agents and reduce silver ions to metallic silver nanoparticles.…”