Energy metabolism of the mussel, Mytilus galloprovincialis, during long-term anoxia

Isani, Gloria; Cattani, Otello; Zurzolo, Margherita; Pagnucco, C.; Cortesi, P.

doi:10.1016/0305-0491(94)00132-e

Cited by 49 publications

(39 citation statements)

References 33 publications

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“…In our study only succinate was detected as an anaerobic end product in the White Sea mussels. In support of our findings, Isani et al (1995) could detect opines only after 14 d of anoxia at +lO°C in M. galloprovincialis.…”

Section: End Productssupporting

confidence: 79%

“…In this context, it should be noted that all literature data concern muscle tissues, while we used the whole mussel. Isani et al (1995) reported 0.21 1.lmol g-' wet wt of PLA under normal conditions for the whole body in M, galloprovincialis, a value much lower than our data. The authors explained this phenomenon by the spawning period at the time of the experiments.…”

Section: High-energy Phosphates and Metabolic Ratecontrasting

confidence: 55%

“…RP,, was, however, lower than previously reported (Zange et al 1989, Isani et al 1995. Thus, for the resting anterior byssus retractor muscle of M, edulis, RPLA in normoxia was not less than 0.8 (Zange et al 1989), while mean values for cultured White Sea mussels were 0.60 and 0.41 for littoral ones.…”

Section: High-energy Phosphates and Metabolic Ratecontrasting

confidence: 44%

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Habitat as a factor involved in the physiological response to environmental anaerobiosis of White Sea Mytilus edulis

Сухотин¹,

Pörtner²

1999

Mar. Ecol. Prog. Ser.

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The ability of blue mussels Mytilus eduljs L to withstand severe environmental hypoxia was studied in mussels from an intertidal population and from a suspended cultured settlement. Specimens were exposed to air for 60 h at +lO°C. Tissues were analysed for the amount of anaerobic metabolic end products, adenylates, phosphagen, and Inorganic phosphate, and for changes in intracellular pH (pHi). Proton balance and Gibb's free energy of ATP hydrolysis were calculated. Under the experimental conditions applied succinate appeared to be thr only anaerobic end product. Under control conditions pH, measured using the homogenate technique ranged between 6.78 and 6.85 in both groups. Very small decreases in pHi were observed after air exposure. Rapid breakdown of ATP and phospho-L-arginine (PLA) was accompanied by the accumulat~on of inorganic phosphate, free AMP and ADP. The Gibb's free energy change of ATP hydrolysis decreased from about -57 to -50 kJ mol-', showing the depletion of energy reserves in the tissues. The calculated ATP turnover rate was higher in intertidal mussels. It is concluded that mussels from the intertidal zone demonstrate lower abilities for metabolic depression under the conditions of air exposure than cultured (sublittoral) ones. The latter are characterised by higher initial PLA content. These differences may be related to a difference in mitochondrial density depending on the habitat.

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Section: End Productssupporting

confidence: 79%

Section: High-energy Phosphates and Metabolic Ratecontrasting

confidence: 55%

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Habitat as a factor involved in the physiological response to environmental anaerobiosis of White Sea Mytilus edulis

Сухотин¹,

Pörtner²

1999

Mar. Ecol. Prog. Ser.

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“…When incubated in near-anoxic conditions, C. fluminea expressed a kind of anaerobiosis frequently described for other bivalves (De Zwaan et al, 1976;Isani et al, 1995). Propionate levels in both the foot (0.13±0.09·µmol·g -1 ·fresh·mass) and the adductor muscles (0.25±0.11·µmol·g -1 ·fresh·mass) were already significantly increased after 1·h of N2 incubation compared to the controls (0.04±0.04·µmol·g -1 ·fresh·mass and 0.05±0.05·µmol·g -1 ·fresh·mass, respectively).…”

Section: Anaerobic End Productsmentioning

confidence: 73%

Energy metabolism and valve closure behaviour in the Asian clamCorbicula fluminea

Ortmann

Grieshaber

2003

Journal of Experimental Biology

131

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SUMMARYSince its invasion of Europe in the early 1980s, the Asian clam Corbicula fluminea has become very abundant in nearly all western river systems. Today this species is one of the most important biomass producers in the River Rhine. Monitoring the valve movements of C. fluminea over a period of 2 years revealed a circadian rhythm in summer,with extended periods (10-12 h) of valve closure, predominantly in the morning hours. Altogether valve movements were very scarce, frequently fewer than four movements per individual per day.Simultaneous measurements of heat dissipation and oxygen consumption(calorespirometry) revealed an intermittent metabolism in the clam. With the onset of valve closure, C. fluminea reduced its metabolic rate to 10%of the standard metabolic rate (SMR) measured when the valves were open. Nevertheless, this depressed metabolism remained aerobic for several hours,enabling the clam to save energy and substrates compared to the requirements of the tenfold higher SMR. Only during long-lasting periods of valve closure(more than 5-10 h) did the clams become anaerobic and accumulate succinate within their tissues (2 μmol g-1 fresh mass). Succinate is transported into the mantle cavity fluid, where it reaches concentrations of 4-6 mmol l-1. Because this succinate-enriched fluid must pass the gills when the valves open again, we suggest that this anaerobic end product is at least partly reabsorbed, thus reducing the loss of valuable substrates during anaerobiosis. Propionate was also produced, but only during experimental N2-incubation, under near-anoxic conditions.The intermittent metabolism of C. fluminea is discussed as an adaption to efficiently exploit the rare food supply, saving substrates by the pronounced metabolic depression during valve closure.

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“…Release from the tissue into EF or ambient water is unlikely because in many marine invertebrates which are known to produce volatile fatty acids during environmental anaerobiosis (e.g. the lugworm Arenicola marina, the peanut worm Sipunculus nudus, and the mussel Mytilus galloprovincialis), considerable amounts of these metabolites are retained by tissues despite high excretion rates (Grieshaber et al 1994;Isani et al 1995;Sommer et al 1997). Furthermore, excretion should be even less pronounced in freshwater-exposed periwinkles due to reduced rate of exchange between tissues and EF, on one side, and ambient water, on the other (Rumsey 1973;Berger 1986; this study).…”

Section: Discussionmentioning

confidence: 99%

Resistance to freshwater exposure in White Sea Littorina spp. I: Anaerobic metabolism and energetics

Sokolova

Bock²,

Pörtner³

2000

Journal of Comparative Physiology B: Biochemical, Systemic, and

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Anaerobic metabolism and changes in the osmotic concentration of extravisceral¯uid were studied in the White Sea periwinkles (Littorina littorea, Littorina saxatilis and Littorina obtusata) during freshwater exposure. Resistance to hypoosmotic stress increased in the order: L. obtusata < L. saxatilis < L. littorea. Our data suggest that osmotic shock is not a primary reason for mortality of the periwinkles under these conditions. During environmental anaerobiosis, considerable succinate accumulation (up to 10±19 lmol g)1 wet weight), and depletion of phosphagen and ATP pools were found in the studied species. Other metabolic end products (alanopine, strombine, lactate, acetate or propionate) were not detected. Succinate accumulation and net ATP breakdown were the fastest in the least resistant species, L. obtusata, and slowest in the most resistant, L. littorea. Rate of ATP turnover decreased during freshwater exposure in L. littorea and L. saxatilis, but not in L. obtusata. Our data suggest that dierential resistance of three studied Littorina spp. to extreme hypoosmotic stress may be related to their dierent abilities to reduce metabolic rate and ATP turnover during sustained anoxia. Species-speci®c variations in anaerobic capacity of Littorina spp. are discussed in relation to their vertical distribution, size and ecology.Key words Salinity stress á Resistance adaptations á Anaerobic metabolism á ATP turnover á Littorina spp.Abbreviations ADP adenosine-5¢-diphosphate á AEC adenylate energy charge á AMP adenosine-5¢-monophosphate á ATP adenosine-5¢-triphosphate á Arg L-arginine á AWI Alfred-Wegener-Institute for Polar and Marine Research á dG/dn Gibbs free energy change á EDTA ethylenediaminetetraacetic acid á EF extravisceral¯uid á HPLC High Performance Liquid Chromatography á HSD honestly signi®cant dierence á w ATP ATP turnover rate á NTA nitrilotriacetic acid á PCA perchloric acid á P i inorganic phosphate á PLA phospho-L-arginine á R PLA relative amount of phosphagen á R ST standardised respiration rate

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Energy metabolism of the mussel, Mytilus galloprovincialis, during long-term anoxia

Cited by 49 publications

References 33 publications

Habitat as a factor involved in the physiological response to environmental anaerobiosis of White Sea Mytilus edulis

Habitat as a factor involved in the physiological response to environmental anaerobiosis of White Sea Mytilus edulis

Energy metabolism and valve closure behaviour in the Asian clamCorbicula fluminea

Resistance to freshwater exposure in White Sea Littorina spp. I: Anaerobic metabolism and energetics

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