Endothelium-dependent and -independent vasodilation of isolated rat aorta induced by caffeine

Hatano, Yoshio; Mizumoto, Kazuhiro; Yoshiyama, Takeshi; Yamamoto, Manabu; Iranami, Hiroshi

doi:10.1152/ajpheart.1995.269.5.h1679

Cited by 24 publications

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“…Caffeine-induced relaxations in rat aortic rings consist partly of a nitric-oxide-dependent component and also of a component involving the inhibition of phosphodiesterases (Hatano et al, 1995).…”

Section: Vascular Effects and Calciummentioning

confidence: 97%

Coffee, caffeine and blood pressure: a critical review

et al. 1999

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Objective: We review the published data relating to intake of coffee and caffeine on blood pressure in man. We also refer to studies on the possible mechanisms of actions of these effects of caffeine. Design: The MEDLINE and Current Contents databases were searched from 1966 to April 1999 using the text words`coffee or caffeine' and`blood pressure or hypertension'. Controlled clinical and epidemiologic studies on the blood pressure effects of coffee or caffeine are reviewed. We also refer to studies on the possible mechanisms of action of these effects of caffeine. Results: Acute intake of coffee and caffeine increases blood pressure. Caffeine is probably the main active component in coffee. The pressor response is strongest in hypertensive subjects. Some studies with repeated administration of caffeine showed a persistent pressor effect, whereas in others chronic caffeine ingestion did not increase blood pressure. Epidemiologic studies have produced contradictory ®ndings regarding the association between blood pressure and coffee consumption. During regular use tolerance to the cardiovascular responses develops in some people, and therefore no systematic elevation of blood pressure in long-term and in population studies can be shown. Conclusions:We conclude that regular coffee may be harmful to some hypertension-prone subjects. The hemodynamic effects of chronic coffee and caffeine consumption have not been suf®ciently studied. The possible mechanisms of the cardiovascular effects of caffeine include the blocking of adenosine receptors and the inhibition of phosphodiesterases.

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Section: Vascular Effects and Calciummentioning

confidence: 97%

Coffee, caffeine and blood pressure: a critical review

et al. 1999

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“…In contrast, endothelium of isolated rat aorta responds to caff eine by promotion of NO synthesis 30 . In the present study, we failed to detect signifi cant shifts in NO production up to 2.5 h after the application of increasing doses of caff eine.…”

Section: Eff Ect Of Caff Eine On No Productionmentioning

confidence: 99%

Caffeine Does Not Modulate Nutritive Blood Flow to Rat Gastric Submucosa - A Microdialysis Study

Cibíček¹,

Zivna²,

Cibicek³

et al. 2008

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Background and Aims:Coff ee irritates the gastric mucosa disrupting its barrier and increasing the risk of peptic ulcers. However, caff eine's contribution to these eff ects has not yet been elucidated. In this study we looked at the local eff ect of caff eine on the microcirculation and nitric oxide production in rats together with systemic marker of oxidative stress malondialdehyde as possible mechanisms whereby caff eine might participate in mucosal barrier impairment.Materials and Methods: Four groups of rats were anesthetized and administered as a bolus four diff erent intraperitoneal doses of caff eine (0, 1, 10 and 50 mg kg -1 b.wt.). The gastric submucosal microcirculation and nitric oxide production were then recorded for 2.5 hours by in situ microdialysis using the fl ow marker ethanol. At the completion of the experiments, plasma caff eine and malondialdehyde levels as well as morphological mucosal injury were determined.Results: There were no major diff erences in the macro-or microscopic pictures of the mucosa among the groups. Local microcirculatory (ethanol out/in ratio) and nitric oxide monitoring failed to demonstrate statistically signifi cant changes as did measurement of plasma malondialdehyde in response to caff eine injections.Conclusions: Caff eine per se seems unlikely to contribute to the gastric mucosal barrier injury associated with coff ee consumption by alterations in nutritive blood fl ow, nitric oxide production or aggravation of systemic oxidative stress. This information is relevant for better understanding of the mechanisms involved in caff eine-mediated infl uences on gastric physiology in relation to the irritant eff ects of coff ee.

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“…Los efectos vasodilatadores arteriales in vitro de la cafeína en modelos animales se han descrito previamente (15)(16)(17)(18); en estos estudios se ha removido el endotelio y los efectos se han explicado por la acción directa sobre las células del músculo liso vascular. El mecanismo de acción vascular directo de la cafeína sobre las células de músculo liso vascular (efecto independiente del endotelio), se ha atribuido a la capacidad de la cafeína de inhibir la fosfodiesterasa cíclica (29,30), que hace que las concentraciones de los nucleótidos cíclicos (AMPc y GMPc) aumenten y ocasionan vasodilatación (31) a través de la reducción de las concentraciones intracelulares de Ca ++ (32)(33)(34).…”

Section: Figuraunclassified

“…El mecanismo de acción vascular directo de la cafeína sobre las células de músculo liso vascular (efecto independiente del endotelio), se ha atribuido a la capacidad de la cafeína de inhibir la fosfodiesterasa cíclica (29,30), que hace que las concentraciones de los nucleótidos cíclicos (AMPc y GMPc) aumenten y ocasionan vasodilatación (31) a través de la reducción de las concentraciones intracelulares de Ca ++ (32)(33)(34). Asimismo, otros estudios han demostrado que la cafeína puede inducir vasodilatación arterial por mecanismos dependientes del endotelio, aumentando las concentraciones de Ca ++ disponibles procedentes del reticulo sarcoplásmico (15,28). El Ca ++ liberado forma un complejo con la calmodulina, lo que activa la expresión y acción de la eNOS, para producir óxido nítrico (NO) mediante la degradación de L-arginina a citrulina (35).…”

Section: Figuraunclassified

“…Los estudios realizados en vasos sanguíneos humanos y de animales demostraron que la ateroesclerosis reduce la relajación vascular obtenida con la acetilcolina (Ach) y el calcio ionóforo (14). Hasta ahora se ha descrito que la cafeína tiene un efecto vasodilatador en las arterias de ratas sanas (15)(16)(17) e hipertensas (17) y en las arterias de conejos sanos (18). Además, se ha encontrado que este efecto es independiente de la denudación in vitro del endotelio (15)(16)(17).…”

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Acción in vitro de la cafeína en anillos de arteria mamaria interna utilizada en cirugía de revascularización cardiaca

et al. 2008

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Introducción. El efecto vasodilatador de la cafeína en las arterias de modelos animales ya ha sido demostrado. Se desconocen estudios con la misma metodología in vitro utilizando arterias humanas. Objetivos. Evaluar los efectos vasoactivos in vitro de la cafeína en la arteria mamaria interna de humanos. Materiales y métodos. Se utilizaron 80 anillos de arteria mamaria interna (n=20 pacientes). La funcionalidad del endotelio se evaluó con acetilcolina a una concentración de 3,16x10 -6 M, de nitroglicerina con dosis acumulativas de 10 -11 M a 10 -4 M y de cafeína con concentraciones acumulativas de 10 -8 M a 10 -4 M. Resultados. La nitroglicerina indujo un porcentaje máximo de relajación de 87,4±12,3%, la cafeína, de 86,9±21,0% en arterias con endotelio funcional y de 71,6±28,6% en arterias con disfunción endotelial. No se encontraron diferencias entre los tres grupos (p=0,289). Tampoco se encontraron diferencias en la EC 50 en arterias con endotelio funcional (1,66x10 -5 ±1,57x10 -5 M) y arterias disfuncionales (7,75x10 -5 ±14,64x10 -5 M). La nitroglicerina resultó más potente que la cafeína (EC 50 = 4,30x10 -9 ±4,35x10 -9 M) (p=0,013). Conclusiones. Aunque la nitroglicerina fue un vasodilatador más potente, la cafeína tuvo un fuerte efecto vasodilatador arterial in vitro independientemente de la funcionalidad del endotelio en arterias humanas.Palabras clave: cafeína/uso terapéutico, revascularización miocárdica, vasodilatación, aorta, ateroesclerosis, endotelio, acetilcolina. In vitro effect of caffeine on internal mammary artery rings used in cardiac revascularization surgeryIntroduction. The vasodilator effect of caffeine in animal models arteries has been demostrated previously. However, studies with the same methodology using human arteries in vitro have not been performed. Objectives. The in vitro vasoactive effects of caffeine was evaluated on human internal mammary arteries. Materials and methods. Internal mammary artery rings were used (n=20). Endothelial function was evaluated with acetylcholine at a concentration of 3.16x10 -6 M, nitroglycerine at cumulative concentrations of 10 -11 M to 10 -4 M and caffeine with cumulative concentrations of 10 -8 M to 10 -4 M. Results. Nitroglycerin produced a maximum relaxation percentage of 87.4±12.3%, caffeine a percentage of 86.9±21.0% in arteries with functional endothelium, and of 71.6±28.6% in arteries with endothelial dysfunction. No differences were detected among the three groups (p=0.289). Similarly, no differences were found between EC 50 in arteries with functional endothelium (1.66x10 -5 ±1.57x10 -5 M) and dysfunctional arteries (7.8 x10 -5 ±14.6 x10 -5 M). Nitroglycerine proved more potent than caffeine (EC 50 = 4.3 x10 -9 ±4.4x10 -9 M) (p=0.013). Conclusions. Although nitroglycerin was a more potent vasodilator, caffeine had a strong arterial vasodilator effect regardless of endothelial function in human arteries.

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Endothelium-dependent and -independent vasodilation of isolated rat aorta induced by caffeine

Cited by 24 publications

References 0 publications

Coffee, caffeine and blood pressure: a critical review

Coffee, caffeine and blood pressure: a critical review

Caffeine Does Not Modulate Nutritive Blood Flow to Rat Gastric Submucosa - A Microdialysis Study

Acción in vitro de la cafeína en anillos de arteria mamaria interna utilizada en cirugía de revascularización cardiaca

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