Endothelins (EDN1, EDN2, EDN3) and their receptors (EDNRA, EDNRB, EDNRB2) in chickens: Functional analysis and tissue distribution

Liu, Haikun; Luo, Qiong; Zhang, Jiannan; Mo, Chunheng; Wang, Yajun; Li, Juan

doi:10.1016/j.ygcen.2019.113231

Cited by 26 publications

(25 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total RNA was extracted from tilapia tissues using RNAzol and dissolved in DEPC-treated H 2 O. These RNA samples were reversely transcribed by Moloney murine leukemia virus (MMLV) reverse transcriptase (Takara) and were used for the PCR amplification of target genes as described in our recent study [53].…”

Section: Total Rna Extraction and Reverse Transcription (Rt)mentioning

confidence: 99%

“…To determine whether fasting can alter hypothalamic AgRP expression, 80 tilapia (♂) of uniform bodyweight (37-38 g) at the growing stage were divided into four groups: two control groups and two fasting groups. After fasting for 3 days and 6 days, the hypothalamus of tilapias were collected for total RNA extraction and subjected to quantitative real-time RT-PCR assay of AgRP expression, as described in our recent study [5,53].…”

Section: Quantitative Real-time Rt-pcr Assay (Qrt-pcr)mentioning

confidence: 99%

See 1 more Smart Citation

Melanocortin Receptor 4 (MC4R) Signaling System in Nile Tilapia

Liu

Deng

Zhang

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

The melanocortin receptor 4 (MC4R) signaling system consists of MC4R, MC4R ligands [melanocyte-stimulating hormone (MSH), adrenocorticotropin (ACTH), agouti-related protein (AgRP)], and melanocortin-2 receptor accessory protein 2 (MRAP2), and it has been proposed to play important roles in feeding and growth in vertebrates. However, the expression and functionality of this system have not been fully characterized in teleosts. Here, we cloned tilapia MC4R, MRAP2b, AgRPs (AgRP, AgRP2), and POMCs (POMCa1, POMCb) genes and characterized the interaction of tilapia MC4R with MRAP2b, AgRP, α-MSH, and ACTH in vitro. The results indicate the following. (1) Tilapia MC4R, MRAP2b, AgRPs, and POMCs share high amino acid identity with their mammalian counterparts. (2) Tilapia MRAP2b could interact with MC4R expressed in CHO cells, as demonstrated by Co-IP assay, and thus decrease MC4R constitutive activity and enhance its sensitivity to ACTH1-40. (3) As in mammals, AgRP can function as an inverse agonist and antagonist of MC4R, either in the presence or absence of MRAP2b. These data, together with the co-expression of MC4R, MRAP2b, AgRPs, and POMCs in tilapia hypothalamus, suggest that as in mammals, ACTH/α-MSH, AgRP, and MRAP2 can interact with MC4R to control energy balance and thus play conserved roles in the feeding and growth of teleosts.

show abstract

Section: Total Rna Extraction and Reverse Transcription (Rt)mentioning

confidence: 99%

Section: Quantitative Real-time Rt-pcr Assay (Qrt-pcr)mentioning

confidence: 99%

Melanocortin Receptor 4 (MC4R) Signaling System in Nile Tilapia

Liu

Deng

Zhang

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…In black chickens, the OCA2 was up-regulated and it may be related to black skin color [173]. In chickens, the endothelins (EDN1, EDN2, and EDN3) and their receptors (EDNRA, EDNRB, and EDNRB2) are involved in the regulation of pigmentation and plumage [218]. The expression of EDNRB2 was signi cantly different between adult black and non-black chicken [219].…”

Section: Expression Of Plumage Development Genesmentioning

confidence: 97%

Transcriptome analysis reveals potential mechanisms and pathways underlying embryonic development with respect to muscle growth and egg production in slow and fast growing chickens

Mogilicherla¹,

Ashwini²,

Chatterjee³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Chicken is one of the important meat sources throughout the globe. Muscle development and egg production are important genetic traits in commercially raising chickens. However, not much information is available in the fast and slow growth of chicken to determine the expression of genes involved in muscle development and egg production in embryo initiation and developmental stages. This study was designed to investigate why improved Aseel (PD4) growing slowly compared with the control broiler (CB), microarray was conducted with the 7th-day embryo and 18th-day thigh muscle of improved Aseel (PD4) and control broiler (CL), respectively.Results: In the differential transcripts screening, all the transcripts obtained by microarray of slow and fast growth groups were screened by fold change ≥1 and false discovery rate (FDR) <0.05. In total, 19022 transcripts were differentially expressed between the 7th-day embryo and 18th-day thigh muscle of improved Aseel compared to the control broiler. Further analysis showed that a high number of transcripts are differentially regulated in the 7th-day improved Aseel embryo (15382) and fewer transcripts were differentially regulated (3640) in the 18th-day thigh muscle of improved Aseel compared to control broiler. In the 7th and 18th-day improved Aseel embryo, 10127, 2102, 5255, and 1538 transcripts were up and down-regulated, respectively. The commonly up and down-regulated transcripts are 545 and 381 between the 7th and 18th-day of embryos. In this study, we have selected 18 Gallus gallus candidate reference genes from NCBI and total RNA was isolated from control broiler, improved Aseel embryo tissues, and studied their expression profiles by real-time quantitative PCR (qPCR). The best housekeeping gene was identified by using geNorm, NormFinder, BestKeeper, Delta CT, and RefFinder analytical software. The result showed that the TFRC gene is the most stable and further it is used for qPCR data normalization. Further, to validate the differentially expressed genes (DEGs) related to muscle growth, myostatin signaling and development, fatty acid metabolism genes in improved Aseel (PD4) and control broiler embryo tissues by qPCR. Conclusion: Our study identified DEGs that regulate myostatin signaling and differentiation pathway, glycolysis and gluconeogenesis, fatty acid metabolism, Jak-STAT, mTOR, and TGF-β signaling pathways, tryptophan metabolism, PI3K-Akt signaling pathways in improved Aseel. The results revealed that the gene expression architecture is present in the improved Aseel exhibiting embryo growth that will help to improve muscle development, differentiation, egg production, as well as protein synthesis in improved Aseel native chicken. Our findings may be used as a model for improving the growth in improved Aseel as well as optimizing the growth in the control broiler.

show abstract

“…Besides, compared with turkey, 43 positively selected genes were significantly enriched in the GO terms of organelle (GO:0043226), extracellular space (GO:0005615), and epithelium migration (GO:0090132), and the pathways of glutathione metabolism (GPX1, GPX2 and GPX4) and thyroid hormone synthesis (GPX1, DUOXA2 and GPX2) (P < 0.05) (Supplementary Table S13 and S14), which were involved in gastrointestinal health, anti-stress, growth development and metabolism. Notably, as a common positive selection gene, EDN1 was reported to participate in many biological process, such as epithelium migration and differentiation, pigmentation and their receptors (EDNRs) widely distributed in various tissues in chicken (H. Liu et al, 2019). These enrichment results indicated that the positively selected genes in peafowl were mainly related to intestinal immunity, anti-stress, growth development and metabolism, and pigmentation compared with turkey and chicken in the evolutionary process, which was beneficial for peafowl to enhance adaptability, improve disease resistance and antistress ability, enrich plumage colour, and better adapt to the living environment of longterm artificial breeding.…”

Section: Genome Synteny and Collinearity Among The Indian Peafowl Chicken And Turkeymentioning

confidence: 99%

A High-Quality Assembly Reveals Genomic Characteristics, 1 Phylogenetic Status and Causal Genes for White Feather of 2 Indian Peafowl

Liu

Chen

Ouyang

et al. 2021

Preprint

View full text Add to dashboard Cite

Background : Indian peafowl ( Pavo cristatus ) attracts people's attention because of the exclusively dazzling phenotypic characteristics. However, little is known about the phenotypic evolution and phylogeny of Indian peafowl at the whole-genome level. So far, there has been no report on the genetic mechanism of the formation of white feather in white feather peafowl. Results : This study assembled a draft genome of Indian peafowl with a genome size of 1.05 Gb (the sequencing depth is 362×), and N50 of the contig and scaffold was up to 6.2 Mb and 11.4 Mb, respectively. Compared with other birds, Indian peafowl changed in terms of metabolism, immunity, skeletal development and feather development, which provided a novel insight into the phenotypic evolution of peafowl, such as the large body size and feather morphologies. It was confirmed that the phylogeny of Indian peafowl was closer to that of turkey than that of chicken. Specially, it was identified that PMEL was a causal gene leading to the formation of white plumage in blue and white feather peafowl. Conclusions: This study provides a peafowl genome with high-quality as well as a novel understanding in the phenotypic evolution and phylogeny of peafowl among other birds. The results contribute a valuable reference genome to the study of the avian genome evolution. In addition, the discovery of the genetic mechanism of white plumage not only is a breakthrough in the exploration of peafowl plumage, but also provides clues and new ideas for further investigations of the avian plumage coloration and artificial breeding in peafowl.

show abstract

Endothelins (EDN1, EDN2, EDN3) and their receptors (EDNRA, EDNRB, EDNRB2) in chickens: Functional analysis and tissue distribution

Cited by 26 publications

References 61 publications

Melanocortin Receptor 4 (MC4R) Signaling System in Nile Tilapia

Melanocortin Receptor 4 (MC4R) Signaling System in Nile Tilapia

Transcriptome analysis reveals potential mechanisms and pathways underlying embryonic development with respect to muscle growth and egg production in slow and fast growing chickens

A High-Quality Assembly Reveals Genomic Characteristics, 1 Phylogenetic Status and Causal Genes for White Feather of 2 Indian Peafowl

Contact Info

Product

Resources

About