Endothelial Cells in Co-culture Enhance Embryonic Stem Cell Differentiation to Pancreatic Progenitors and Insulin-Producing Cells through BMP Signaling

Talavera-Adame, Dodanim; Wu, Guey-Shuang; He, Yao; Ng, Tina T.; Gupta, Ankur; Kurtovic, Silvia; Hwang, Jae Youn; Farkas, Daniel L.; Dafoe, Donald C.

doi:10.1007/s12015-011-9232-z

Cited by 49 publications

(52 citation statements)

References 53 publications

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“…The role of BMPs in pancreas development has also been explored previously [72,93] . We demonstrated that HMECs or mouse dermis as well as mouse AECs expressed BMPs and that BMP-2 and BMP-4 increased the phosphorylation levels of SMAD1,5,8 in pancreatic progenitors and beta-like cells derived from mouse ESCs [52,53,94] . These findings together with previous works pointed out the important role of ECs in beta-cell differentiation in vitro.…”

Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 90%

“…Although some epithelial cells appeared, no cells derived from endoderm were identified [90] . Then, studies using co-cultures between human microvascular ECs (HMECs) and mouse EBs were performed [52,53,91] . In these studies, ECs from human dermis were able to induce differentiation of mouse EBs to pancreatic progenitors and insulin-producing cells [52] .…”

Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 99%

“…Then, studies using co-cultures between human microvascular ECs (HMECs) and mouse EBs were performed [52,53,91] . In these studies, ECs from human dermis were able to induce differentiation of mouse EBs to pancreatic progenitors and insulin-producing cells [52] . Furthermore, BMP-2/-4 were involved in this differentiation process as evaluated by the effects of agonists (recombinant BMPs), and specific antagonists of BMP bioactivities (Noggin, Chordin).…”

Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 99%

“…BMPs are members of the TGF-b superfamily [92] . In addition to the effects of BMP antagonists, we explored the levels of phosphorylation of SMAD1, 5, 8 in cells that expressed proinsulin [52,53] . The role of BMPs in pancreas development has also been explored previously [72,93] .…”

Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 99%

“…External ECs produce factors such as BMPs and other EC-derived factors that promote upregulation of endogenous BMPs in still unknown target cells (possibly mesenchymal or internal ECs). These BMPs together with other unknown factors may promote differentiation of multipotent cells (MC) toward beta-like cells (BC) and other cell lineages (OCL) [52,53] .…”

Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 99%

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Endothelium-derived essential signals involved in pancreas organogenesis

Talavera-Adame

Dafoe

2015

WJEM

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Endothelial cells (ECs) are essential for pancreas differentiation, endocrine specification, and endocrine function. They are also involved in the physiopathology of type 1 and type 2 diabetes. During embryogenesis, aortic ECs provide specific factors that maintain the expression of key genes for pancreas development such as pancreatic and duodenal homeobox-1. Other unknown factors are also important for pancreatic endocrine specification and formation of insulin-producing beta cells. Endocrine precursors proliferate interspersed with ductal cells and exocrine precursors and, at some point of development, these endocrine precursors migrate to pancreatic mesenchyme and start forming the islets of Langerhans. By the end of the gestation and close to birth, these islets contain immature beta cells with the capacity to express vascular endothelial growth factor and therefore to recruit ECs from the surrounding microenvironment. ECs in turn produce factors that are essential to maintain insulin secretion in pancreatic beta cells. Once assembled, a cross talk between endocrine cells and ECs maintain the integrity of islets toward an adequate function during the whole life of the adult individual. This review will focus in the EC role in the differentiation and maturation of pancreatic beta cells during embryogenesis as well as the current knowledge about the involvement of endothelium to derive pancreatic beta cells in vitro from mouse or human pluripotent stem cells.

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Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 90%

Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 99%

Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 99%

Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 99%

Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 99%

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Endothelium-derived essential signals involved in pancreas organogenesis

Talavera-Adame

Dafoe

2015

WJEM

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Anisotropic Crystalline Protein Nanolayers as Multi‐Functional Biointerface for Patterned Co‐Cultures of Adherent and Non‐Adherent Cells in Microfluidic Devices

Rothbauer

Ertl

Theiler

et al. 2014

Adv Materials Inter

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The spatial arrangement of cells in their microenvironment is known to significantly influence cellular behavior, thus making the control of cellular organization an important parameter of in vitro co‐culture models. However, recent advances in micropatterning co‐culture methods within biochips do not address the simultaneous cultivation of anchorage‐dependent and non‐adherent cells. To address this methodological gap we combine S‐layer technology with microfluidics to pattern co‐cultures to study the cell‐to‐cell and cell‐to‐surface interactions under physiologically relevant conditions. We exploit the unique self‐assembly properties of SbpA and SbsB S‐layers to create an anisotropic protein nanobiointerface on‐chip with spatially‐defined cytophilic (adhesive) and cytophobic (repulsive) properties. While microfluidics control physical parameters such as shear force and flow velocities, our anisotropic protein nanobiointerface regulates the biological aspects of the co‐culture method including biocompatibility, biostability, and affinity to non‐adherent cells. The reliability and reproducibility of our microfluidic co‐culture strategy based on laminar flow patterned protein nanolayers is envisioned to advance in vitro models for biomedical research.

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Short exposure to collagenase and coculture with mouse embryonic pancreas improve human dermal fibroblast culture

Pandamooz

Hadipour

Akhavan‐Niaki

et al. 2012

Biotech and App Biochem

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The dermal fibroblast as a major component of connective tissue has attracted much attention in the past few years, and application of these very fast growing cells in several fields has been intensively studied. Isolating dermal fibroblasts is an appropriate way to expand these fast growing cells in vitro. Although using a dissociated fibroblast culture method is more convenient than skin explant culture, its enzymatic digestion is critical because a large number of cells can be lost over prolonged exposure to collagenase. This study was performed to increase the number of viable cells after digestion of fresh human foreskin of donors aged from 1 to 3 months with collagenase and also by to design a coculture system for resuscitation of the injured fibroblast. Our results demonstrate that we can maximize cell yield while maintaining cell viability by cutting the specimens into very small pieces (1-2 mm³) after removing the epidermal layer with dispase II and also by collecting released cells every 20 Min subsequent to digesting the dermal layer with collagenase. Moreover, our data strongly indicate that coculturing of isolated fibroblasts with embryonic pancreas explants can enhance the rate of proliferation in cultured fibroblasts.

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Endothelial Cells in Co-culture Enhance Embryonic Stem Cell Differentiation to Pancreatic Progenitors and Insulin-Producing Cells through BMP Signaling

Cited by 49 publications

References 53 publications

Endothelium-derived essential signals involved in pancreas organogenesis

Endothelium-derived essential signals involved in pancreas organogenesis

Anisotropic Crystalline Protein Nanolayers as Multi‐Functional Biointerface for Patterned Co‐Cultures of Adherent and Non‐Adherent Cells in Microfluidic Devices

Short exposure to collagenase and coculture with mouse embryonic pancreas improve human dermal fibroblast culture

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