Endoscopic urease sensor system for detecting Helicobacter pylori on gastric mucosa

Satô, Tadashi; Fujino, Masayuki; Kojima, Yuichiro; Ohtsuka, Hiroyuki; Ohtaka, Masahiko; Kubo, Kanenobu; Nakamura, Toshiya; Morozumi, Atsuro; Nakamura, Michihiro; Hosaka, Hiroko

doi:10.1016/s0016-5107(99)70442-6

Cited by 11 publications

(5 citation statements)

References 9 publications

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“… 14 The rapid urease test can also be performed per‐endoscopically with the use of a pH sensitive biosensor, giving a sensitivity and specificity of 92% and 95%, respectively; this test takes one minute to perform. 15 …”

Section: Invasive Diagnosismentioning

confidence: 99%

Invasive and non‐invasive tests for Helicobacter pylori infection

Vaira

Holton

Menegatti

et al. 2000

Aliment Pharmacol Ther

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Summary There are two general ways in which a diagnosis of infection by Helicobacter pylori can be made: by using either an invasive or non‐invasive procedure. The invasive procedures involve an endoscopy and biopsy. A biopsy is essential because often the mucosa may appear macroscopically normal but nevertheless be inflamed. A biopsy is obtained by histological examination, culture, polymerase chain reaction or detection of the presence of urease activity in biopsy material. The non‐invasive tests that can be used to diagnose the infection are serology, detection of labelled metabolic products of urea hydrolysis in the breath (13CO2, 14CO2), the urine or the blood, and detection of H, pylori antigen in a stool specimen. At present no single test can be relied upon to detect definitely colonization by H. pylori, and a combination of two is recommended if this is feasible. The choice of the test to be used is not straightforward and may vary according to the clinical condition and local expertise.

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Section: Invasive Diagnosismentioning

confidence: 99%

Invasive and non‐invasive tests for Helicobacter pylori infection

Vaira

Holton

Menegatti

et al. 2000

Aliment Pharmacol Ther

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“…4 that the change in potential is directly proportional to urease concentration over the tested range (1 to 50 U/mL). Minimum urease activity values quoted by T. Sato et al [6] for H. pylori positive patients equate to approximately 1.5 U/mL, near the gastric mucus. This is within the linear range determined here (1-50 U/mL) and suggests the sensing protocol developed could be suitable for H. pylori detection.…”

Section: Urease Measurement and H Pylori Detectionmentioning

confidence: 86%

“…pH sensors have been used for the measurement of urease activity [5]. T. Sato et al [6] developed a pH sensitive field effect transistor (pH-FET) mounted on an endoscope for H. pylori detection. Their strategy involves placing a pH-FET near the gastric mucus during endoscopy, replacing the gastric fluid around the sensor with a buffered ammonium solution, then measuring the change in pH that occurs due to the hydrolysis of urea by H. pylori present in the mucus.…”

Section: Introductionmentioning

confidence: 99%

Rapid measurement of urease activity using a potentiometric RuO2 pH sensor for detection of Helicobacter pylori

Lonsdale

Maurya

Wajrak

et al. 2017

Sensors and Actuators B: Chemical

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“…Kohli et al developed an endoscopic urease sensor system for detecting H. pylori on the gastric mucosa 11 . We made improvements in both hardware and software of the system for the practical application; sensitivity and specificity of the system were calculated as 92.9% and 95.7%, respectively 12 . Recently Urita and Miki reported a new modification of the urea breath test using gastric gas collected by endoscopy as a sample.…”

Section: Biopsy Site Number and Biopsy Forcepsmentioning

confidence: 99%

RECENT PROGRESS IN ENDOSCOPY‐BASED DIAGNOSIS OF HELICOBACTER PYLORI INFECTION

Satô

Fujino

Kitahara

et al. 2001

Digestive Endoscopy

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Recently the Japanese Society for Helicobacter Research (JSHR) published the guideline for the diagnosis and treatment of H. pylori infection. 2 It is estimated that the Japanese Ministry of Health and Welfare will approve the diagnosis and treatment of H. pylori infection as the health insurancecovered medical services in the near future. The aim of this paper is to review current endoscopy-based diagnostic modalities for H. pylori infection and to emphasize their preferential indications, taking into account the particular settings in which a diagnosis is to be made. BIOPSY SITE, NUMBER AND BIOPSY FORCEPSIt is now well-recognized that persistent H. pylori infection develops atrophic gastritis leading to intestinal metaplasia. Intestinal metaplasia and patchy distribution of the bacteria are thought to be factors causing false-negative diagnoses of H. pylori infection. There are several reports regarding biopsy sites and number for the accurate diagnosis of H. pylori infection. Hazzel et al. reported that it was necessary to take biopsies from both the antrum and the body, as H. pylori might be found in either of them. 5 Genta and Graham reported that two antral biopsy specimens, one from lesser curvature and one from greater curvature, yield virtually 100% sensitivity for the histological detection of H. pylori infection. They also recommended three biopsy specimens (one from each of lesser curvature and greater curvature of the antrum and greater curvature of the corpus) as a routine evaluation of H. pylori infection, because no patients having extensive atrophic gastritis with intestinal metaplasia were included in their series. 3 Satoh et al. indicated that the Digestive Endoscopy (2001) 13, 3-6Numerous invasive and non-invasive tests are available in the detection of Helicobacter pylori. Endoscopy-based tests that include rapid urease test, histological examination and culture are important generally in the assessment of H. pylori status before eradication therapy. Recently, several new endoscopy-based diagnostic methods have been developed aiming at rapid and accurate detection of the organisms. It would be possible to diagnose H. pylori infection in treated patients by using these new highly sensitive tests. Although the diagnosis of H. pylori infection itself is possible by using non-invasive diagnostic tests, endoscopy-based tests provide not only the diagnosis of the organisms, but also the exclusive information such as treatment indications and the susceptibility for the antimicrobial drugs. Recently, new triple therapy including clarithromycin has been widely performed in Japan. Along with an increase in the prevalence of the antibiotic-resistant strains, culture may become a more important diagnostic method in the future. The inappropriate application of the tests may increase the potential risk of the misdiagnosis and the treatment failures. The diagnostic method should be selected by taking into account the circumstances in which a diagnosis is to be performed.

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Endoscopic urease sensor system for detecting Helicobacter pylori on gastric mucosa

Cited by 11 publications

References 9 publications

Invasive and non‐invasive tests for Helicobacter pylori infection

Invasive and non‐invasive tests for Helicobacter pylori infection

Rapid measurement of urease activity using a potentiometric RuO2 pH sensor for detection of Helicobacter pylori

RECENT PROGRESS IN ENDOSCOPY‐BASED DIAGNOSIS OF HELICOBACTER PYLORI INFECTION

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