Endophytic fungi from selected varieties of soybean (Glycine max L. Merr.) and corn (Zea mays L.) grown in an agricultural area of Argentina

Abstract

Endophytic fungi are ubiquitous and live within host plants without causing any noticeable symptoms of disease. Little is known about the diversity and function of fungal endophytes in plants, particularly in economically important species. The aim of this study was to determine the identity and diversity of endophytic fungi in leaves, stems and roots of soybean and corn plants and to determine their infection frequencies. Plants were collected in six areas of the provinces of Buenos Aires and Entre Ríos (Argentina) two areas were selected for sampling corn and four for soybean. Leaf, stem and root samples were surface-sterilized, cut into 1cm(2) pieces using a sterile scalpel and aseptically transferred to plates containing potato dextrose agar plus antibiotics. The species were identified using both morphological and molecular data. Fungal endophyte colonization in soybean plants was influenced by tissue type and varieties whereas in corn plants only by tissue type. A greater number of endophytes were isolated from stem tissues than from leaves and root tissues in both species of plants. The most frequently isolated species in all soybean cultivars was Fusarium graminearum and the least isolated one was Scopulariopsis brevicaulis. Furthermore, the most frequently isolated species in corn plants was Aspergillus terreus whereas the least isolated one was Aspergillus flavus. These results could be relevant in the search for endophytic fungi isolates that could be of interest in the control of agricultural pests.

Endophytic Mycobiota of Jingbai Pear Trees in North China

“…Endophytic fungi have received extraordinary attention [4,7,8] due to the ability to produce bioactive products [9][10][11], promote host growth, resist environmental stress [12,13], and even litter decomposition [14]. Many studies have focused on fungal endophytes from agricultural crops, such as wheat, soybeans and tomatoes [15,16], medicinal plants [4,17] and forest trees [18,19]. Although, endophytic fungi of apple and kiwifruit [20,21], wild bananas [22], sour cherry [23] and plums [24] have been reported, information on endophytes from fruit trees is still very limited [23,25].…”

Peer Review #1 of "Endophytic fungal communities associated with field-grown soybean roots and seeds in the Huang-Huai region of China (v0.1)"

“…However, these genera have been reported to be major pathogens in other plants such as wheat (Kriuchkova 2013;Perello & Moreno 2005). Among the endophytic fungi isolated from soybean, a high dominance was observed in the roots of Fusarium, and Fusarium has been isolated from various parts of the soybean plant (Pimentel et al 2006;Russo et al 2016;Impullitti & Malvick 2013), which can be PeerJ reviewing PDF | (2018:02:25939:1:1:CHECK 12 Apr 2018)…”

“…The endophytic fungal communities of soybeans may be influenced by many factors, such as the type of tissue sampled and the location in which they are grown. Greater numbers of endophytes were isolated from stem tissues than from leaves and root tissues in both soybeans and corn (Russo et al 2016). In the medicinal plant Kadsura angustifoli, the fungal species diversity differed significantly between stems and roots, with roots containing a greater diversity than stems (Huang et al 2015).…”

“…In Argentina, 11 genera of fungal endophytes were identified in soybean leaves, stems, and roots. Fungal endophytic colonization in soybean plants was influenced by tissue type, with a greater number of endophytes isolated from stems than from leaves and roots (Russo et al 2016). In the U.S., endophytes in the stems of soybean plants were isolated using culture-dependent (CD) and culture-independent (CI) colony isolation methods, with 12 and 6 genera identified, respectively (Impullitti & Malvick 2013).…”

ANALYSIS OF MYCOBIOME IN DAMAGED POTATO (Solanum tu-berosum L.) LEAVES BY USING METAGENOMIC APPROACHES

“…Альтернариоз и фитофтороз распространены во всех картофелеводческих регионах, проблемой борьбы с ними занимаются ученые в России и за рубежом. В частности, известны исследования филлопланы (3,12), эндофитных (13,14) и почвенных грибных сообществ (15,16), а также непосредственно участков растений, пораженных микозами (17,18); в результате были обнаружены возбудители фитофтороза, альтернариоза и других микологических заболеваний. Активно изучается воздействие на патогены различных агротехнических методов (15) и фунгицидов (8,10,19), которые могут использоваться в комбинации с другими средствами борьбы (20), например совместно с культуральной жидкостью бактерии Klebsiella planticola (21).…”