Endophytic bacterial diversity in banana 'Prata Anã' (Musa spp.) roots

Abstract

The genetic diversity of endophytic bacteria in banana ‘Prata Anã’ roots was characterized. Two hundred and one endophytic bacteria were isolated, 151 of which were classified as Gram-positive and 50 as Gram-negative. No hypersensitivity response was observed in any of the isolates. The rep-PCR technique generated different molecular profiles for each primer set (REP, ERIC and BOX). Fifty readable loci were obtained and all of the fragments were polymorphic. Amplified ribosomal DNA restriction analysis (ARDRA) of the isolates based on cleavage with four restriction enzymes yielded 45 polymorphic bands and no monomorphic bands. PCR amplified the nifH gene in 24 isolates. 16S rDNA sequencing of the 201 bacterial isolates yielded 102 high-quality sequences. Sequence analyses revealed that the isolates were distributed among ten bacterial genera (Agrobacterium, Aneurinibacillus, Bacillus, Enterobacter, Klebsiella, Lysinibacillus, Micrococcus, Paenibacillus, Rhizobium and Sporolactobacillus) and included 15 species. The greatest number of isolates belonged to the genus Bacillus. The bacteria identified in this study may be involved in promoting growth, phosphate solubilization, biological control and nitrogen fixation in bananas.

Phosphate solubilization by endophytic bacteria isolated from banana trees

“…roots characterized by Souza et al (2013) were assessed. Partial sequences of the bacterial 16S rRNA region were deposited in the EMBL (European Molecular Biology Laboratory)/ GenBank database (Table I).…”

Técnicas para el aislamiento, identificación y caracterización molecular de cepas de Ralstonia solanacearum relacionadas a Moko del plátano

“…However, even in this medium, isolating this bacteria is difficult, due to the growth of other microorganisms (Champoiseau et al, 2009;Kalpage y De Costa, 2015), particularly bacteria of the genera Klebsiella, Erwinia, and Pseudomonas. These three gram-negative bacteria, like other species of Ralstonia, such as Ralstonia mannitolilytica, are majority endophytic in banana plants (Thomas et al, 2008;Ganen et al, 2009;Souza et al, 2013). These bacteria are common contaminants in R. solanacearum plantations, since they present a faster growth and similar microscopic and colonial morphologies (French et al, 1995;Nasim, 2011).…”

“…This report describes a strategy that makes the isolation and genetic characterization of R. solanacearum easier, from banana diseased with Moko. (Thomas et al, 2008;Ganen et al, 2009;Souza et al, 2013). Estas bacterias son contaminantes comunes en los cultivos de R. solanacearum debido a que presentan mayor capacidad de crecimiento y muestran morfologías microscópicas y coloniales similares (French et al, 1995;Nasim, 2011).…”

“…Ramesh et al, (2009) point out that genus Pseudomonas is opportunist during the colonization of the host by R. solanacearum. Along with Pseudomonas, the bacteria Klebsiella, Erwinia, and other species of the genus Ralstonia, such as Ralstonia mannitolilytica, are mostly endophytic of the banana (Thomas et al, 2008;Ganen et al, 2009;Souza et al, 2013). The bacteria recovered from the 3 samples that produced faint lines on the ImmunoStrips presented fluorescence on the B-King medium and did not amplify in any of the PCR diagnosis reactions for R. solanacearum.…”

“…The identity of the R. solanacearum strains was confirmed by sequencing the amplified fragment (Thomas et al, 2008;Ganen et al, 2009;Souza et al, 2013). Las bacterias recuperadas de las 3 muestras que dieron las líneas tenues en las inmunotiras presentaron fluorescencia sobre el medio B-King y no amplificaron en ninguna de las reacciones de PCR de diagnóstico para R. solanacearum.…”