Endocrine regulation of carbonate precipitate formation in marine fish intestine by Stanniocalcin and PTHrP

Gregório, Sílvia F.; Carvalho, Edison Samir Mascarelhas; Campinho, Marco A.; Power, Deborah M.; Canário, Adelino V. M.; Fuentes, Juan

doi:10.1242/jeb.098517

Cited by 16 publications

(16 citation statements)

References 42 publications

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“…It has been well demonstrated that intestinal bicarbonate and calcium concentrations, the substrates required for CaCO 3 formation, are controlled via various mechanisms, which can affect the rate of CaCO 3 formation. Regulators of intestinal bicarbonate include the guanylin peptides 31 and prolactin 32 , where calciotropic hormones such as parathyroid hormone-related protein (PTHrP) and stanniocalcin (STC) can modify both luminal bicarbonate and calcium concentrations 33 34 . Additionally, the presence of calcium itself can modify the rate of intestinal bicarbonate secretion 35 .…”

Section: Discussionmentioning

confidence: 99%

A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine

Schauer

LeMoine

Pelin

et al. 2016

Sci Rep

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Marine teleost fish produce CaCO3 in their intestine as part of their osmoregulatory strategy. This precipitation is critical for rehydration and survival of the largest vertebrate group on earth, yet the molecular mechanisms that regulate this reaction are unknown. Here, we isolate and characterize an organic matrix associated with the intestinal precipitates produced by Gulf toadfish (Opsanus beta). Toadfish precipitates were purified using two different methods, and the associated organic matrix was extracted. Greater than 150 proteins were identified in the isolated matrix by mass spectrometry and subsequent database searching using an O. beta transcriptomic sequence library produced here. Many of the identified proteins were enriched in the matrix compared to the intestinal fluid, and three showed no substantial homology to any previously characterized protein in the NCBI database. To test the functionality of the isolated matrix, a micro-modified in vitro calcification assay was designed, which revealed that low concentrations of isolated matrix substantially promoted CaCO3 production, where high concentrations showed an inhibitory effect. High concentrations of matrix also decreased the incorporation of magnesium into the forming mineral, potentially providing an explanation for the variability in magnesium content observed in precipitates produced by different fish species.

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Section: Discussionmentioning

confidence: 99%

A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine

Schauer

LeMoine

Pelin

et al. 2016

Sci Rep

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“…There are recent studies that suggest a relationship between calcium transport and HCO 3 − secretion in the intestine of marine fish in terms of regulation by endocrine factors or environmental effects [ 5 , 10 , 23 , 35 , 49 , 50 ]. The single common factor to all studies above cited is calcium.…”

Section: Discussionmentioning

confidence: 99%

“…Drinking is necessary to compensate osmotic water losses [ 1 , 2 , 3 ], therefore water replacement by drinking becomes essential to sustain osmotic regulation [ 4 ]. Several studies have reported the involvement of endocrine and environmental factors in the regulation of the amount of water ingestion by seawater fish [ 4 , 5 , 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Several hormones, such as stanniocalcin (STC), calcitonin, somatolactin (SL), parathyroid hormone-related protein (PTHrP) and, parathyroid hormone (PTH) are linked in the control of calcium transport in fish [ 5 , 7 , 30 , 31 , 32 , 33 , 34 ]. And recently, in the sea bream [ 10 , 35 ] it was demonstrated that the internal modulation of epithelial HCO 3 − secretion and the associated carbonate precipitation are under endocrine control highlighting a novel physiological role for calcium regulation.…”

Section: Introductionmentioning

confidence: 99%

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Regulation of Bicarbonate Secretion in Marine Fish Intestine by the Calcium-Sensing Receptor

Gregório

Fuentes

2018

IJMS

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In marine fish, high epithelial intestinal HCO3− secretion generates luminal carbonate precipitates of divalent cations that play a key role in water and ion homeostasis. The present study was designed to expose the putative role for calcium and the calcium-sensing receptor (CaSR) in the regulation of HCO3− secretion in the intestine of the sea bream (Sparus aurata L.). Effects on the expression of the CaSR in the intestine were evaluated by qPCR and an increase was observed in the anterior intestine in fed fish compared with unfed fish and with different regions of intestine. CaSR expression reflected intestinal fluid calcium concentration. In addition, anterior intestine tissue was mounted in Ussing chambers to test the putative regulation of HCO3− secretion in vitro using the anterior intestine. HCO3− secretion was sensitive to varying calcium levels in luminal saline and to calcimimetic compounds known to activate/block the CaSR i.e., R 568 and NPS-2143. Subsequent experiments were performed in intestinal sacs to measure water absorption and the sensitivity of water absorption to varying luminal levels of calcium and calcimimetics were exposed as well. It appears, that CaSR mediates HCO3− secretion and water absorption in marine fish as shown by responsiveness to calcium levels and calcimimetic compounds.

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“…In pufferfish and zebrafish two pth3 were identified and cloned, pth3a/pth3b (Power et al, 2000 ; Canario et al, 2006 ; Yan et al, 2012 ), however only pth3a has been identified in seabream (Flanagan et al, 2000 ). Recent studies have outlined the importance of Pth3 in fish ion balance (Gregório et al, 2014 ). The increase of circulating Pth3 in seabream stimulates the calcium uptake through gills and intestine (Guerreiro et al, 2001 ; Abbink et al, 2006 ; Fuentes and Figueiredo, 2006 ).…”

Section: Pth3 (Pthlh)mentioning

confidence: 99%

PTH Reloaded: A New Evolutionary Perspective

et al. 2017

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The parathyroid hormone (PTH) family is a group of structurally-related secreted peptides involved in bone mineral homeostasis and multitude of developmental processes in vertebrates. These peptides mediate actions through PTH receptors (PTHRs), which belong to the transmembrane G protein-coupled receptor group. To date, genes encoding for PTH and PTHR have only been identified in chordates, suggesting that this signaling pathway may be an evolutionary innovation of our phylum. In vertebrates, we found up to six PTH and three PTHR different paralogs, varying in number between mammals and teleost fishes due to the different rounds of whole-genome duplication and specific gene losses suffered between the two groups of animals. The diversification of the PTH gene family has been accompanied by both functional divergence and convergence, making sometimes difficult the comparison between PTH peptides of teleosts and mammals. Here, we review the roles of all Pth peptides in fishes, and based on the evolutionary history of PTH paralogs, we propose a new and simple nomenclature from PTH1 to PTH4. Moreover, the recent characterization of the Pth4 in zebrafish allows us to consider the prominent role of the brain-to-bone signaling pathway in the regulation of bone development and homeostasis. Finally, comparison between PTH peptides of fish and mammals allows us to discuss an evolutionary model for PTH functions related to bone mineral balance during the vertebrate transition from an aquatic to a terrestrial environment.

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Endocrine regulation of carbonate precipitate formation in marine fish intestine by Stanniocalcin and PTHrP

Cited by 16 publications

References 42 publications

A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine

A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine

Regulation of Bicarbonate Secretion in Marine Fish Intestine by the Calcium-Sensing Receptor

PTH Reloaded: A New Evolutionary Perspective

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