Endocarditis lenta durch Lactobacillus salivarius subsp. salicinicus

Berger, Ulrich; Lutz, Philipp; Sievers, Corey; Fk, Trefz

doi:10.1055/s-0028-1104270

Cited by 7 publications

(3 citation statements)

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“…contributing to biofilm formation on surfaces such as teeth, and aiding in immune system evasion when exposed to blood by binding fibrinogen. A case report by Berger et al (1976) attributes a case of endocarditis in a 10-year-old girl after the extraction of two teeth to L. salivarius.…”

Section: Discussionmentioning

confidence: 99%

“…L. salivarius strains have shown great potential as probiotics (Aiba et al ., 1998; O'Mahony et al ., 2001; Olivares et al ., 2006; Neville and O'Toole, 2010). However, there have been reports of Lactobacillus species, often in immunocompromised patients, being implicated in endocarditis, bacteraemic cholecystitis and sepsis (Berger et al ., 1976; Gasser, 1994; Husni et al ., 1997; Woo et al ., 2002). Although rare, it is also likely that L. salivarius infection is under‐reported due to ambiguity of results obtained with traditional carbohydrate fermentation profiles (Song et al ., 1999; Woo et al ., 2002).…”

Section: Discussionmentioning

confidence: 99%

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Fibrinogen‐binding and platelet‐aggregation activities of a Lactobacillus salivarius septicaemia isolate are mediated by a novel fibrinogen‐binding protein

Collins

Pijkeren

Svensson

et al. 2012

Molecular Microbiology

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Summary The marketplace for probiotic foods is burgeoning, measured in billions of euro per annum. It is imperative, however, that all bacterial strains are fully assessed for human safety. The ability to bind fibrinogen is considered a potential pathogenicity trait that can lead to platelet aggregation, serious medical complications, and in some instances, death. Here we examined strains from species frequently used as probiotics for their ability to bind human fibrinogen. Only one strain (CCUG 47825), a Lactobacillus salivarius isolate from a case of septicaemia, was found to strongly adhere to fibrinogen. Furthermore, this strain was found to aggregate human platelets at a level comparable to the human pathogen Staphylococcus aureus. By sequencing the genome of CCUG 47825, we were able to identify candidate genes responsible for fibrinogen binding. Complementing the genetic analysis with traditional molecular microbiological techniques enabled the identification of the novel fibrinogen receptor, CCUG_2371. Although only strain CCUG 47825 bound fibrinogen under laboratory conditions, homologues of the novel fibrinogen binding gene CCUG_2371 are widespread among L. salivarius strains, maintaining their potential to bind fibrinogen if expressed. We highlight the fact that without a full genetic analysis of strains for human consumption, potential pathogenicity traits may go undetected.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fibrinogen‐binding and platelet‐aggregation activities of a Lactobacillus salivarius septicaemia isolate are mediated by a novel fibrinogen‐binding protein

Collins

Pijkeren

Svensson

et al. 2012

Molecular Microbiology

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“…L. salivarius is part of the normal flora in the human oral cavity, gastrointestinal tract, and genitourinary tract. It has been reported to cause only dental caries and a case of endocarditis in humans (1,5). Several reasons may explain why the organism detected in the present study was found in pure culture.…”

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confidence: 38%

Identification by 16S rRNA Gene Sequencing of Lactobacillus salivarius Bacteremic Cholecystitis

et al. 2002

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An anaerobic, nonsporulating, gram-positive bacterium was isolated from blood and bile pus cultures of a 70-year-old man with bacteremic acute cholecystitis. The API 20A system showed that it was 70% Actinomyces naeslundii and 30% Bifidobacterium species, whereas the Vitek ANI system and the ATB ID32A Expression system showed that it was "unidentified." The 16S rRNA gene of the strain was amplified and sequenced. There were 3 base differences between the nucleotide sequence of the isolate and that of Lactobacillus salivarius subsp. salivarius or L. salivarius subsp. salicinius, indicating that the isolate was a strain of L. salivarius. The patient responded to cholecystectomy and a 2-week course of antibiotic treatment. Identification of the organism in the present study was important because the duration of antibiotic therapy would have been entirely different depending on the organism. If the bacterium had been identified as Actinomyces, penicillin for 6 months would have been the regimen of choice. However, it was Lactobacillus, and a 2-week course of antibiotic was sufficient.Identification of anaerobic, nonsporulating, gram-positive bacteria in the clinical microbiology laboratory is traditionally performed by isolation of the organism and phenotypic study of the organism by elucidation of its morphological and biochemical characteristics and metabolic end products. However, these methods have two major drawbacks. First, for organic acid analysis by gas chromatography, special equipment and expertise are required but are generally not available in most routine clinical microbiology laboratories. Second, we are occasionally faced with organisms with biochemical characteristics that do not match the patterns for any known genus and species.Since the discovery of PCR and DNA sequencing, comparison of the gene sequences of bacterial species showed that the 16S rRNA gene is highly conserved within a species and among species of the same genus and hence can be used as the new "gold standard" for determination of the species of bacteria. By use of this new standard, phylogenetic trees, based on base differences between species, are constructed; and bacteria are classified and reclassified into new genera (12, 13). Furthermore, noncultivable organisms and organisms with ambiguous biochemical profiles can be classified and identified (15,16). Recently, we have reported on the application of 16S rRNA gene sequencing to the identification of clinical isolates with ambiguous biochemical profiles (23,24,26,27) and a bacterium that was noncultivable (6). Here, we report on the application of such a technique to the identification of a strain of Lactobacillus salivarius isolated from the blood and gallbladder pus of a patient with acute cholecystitis and bacteremia and discuss the significance of identification of the organism.All clinical data were collected prospectively, as described in our previous publication (9). Clinical specimens were collected and handled by standard protocols. The Vitek ANI system (bioMerieux Vi...

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Lactobacillus gasseri as the cause of septic urinary infection

Dickgiesser¹,

Weiss

Fritsche³

1984

Infection

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A 66-year-old patient developed severe urinary stasis due to a concrement in his right ureter; foudroyant septicemia followed. Lactobacillus gasseri was grown from pyoid urine from the right renal pelvis and in two blood cultures, thus suggesting septic urinary infection caused by lactobacilli. The infection was cured by catheterisation of right ureter and antibiotic treatment with cefotaxime and amoxicillin. The concrement was removed by dissolution. Diabetes and urinary stasis appear to be the main predisposing factors for this exceptional case of septic urinary infection caused by L. gasseri.

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Endocarditis lenta durch Lactobacillus salivarius subsp. salicinicus

Cited by 7 publications

References 0 publications

Fibrinogen‐binding and platelet‐aggregation activities of a Lactobacillus salivarius septicaemia isolate are mediated by a novel fibrinogen‐binding protein

Fibrinogen‐binding and platelet‐aggregation activities of a Lactobacillus salivarius septicaemia isolate are mediated by a novel fibrinogen‐binding protein

Identification by 16S rRNA Gene Sequencing of Lactobacillus salivarius Bacteremic Cholecystitis

Lactobacillus gasseri as the cause of septic urinary infection

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