Encapsulated Pseudomonas putida for phenol biodegradation: Use of a structural membrane for construction of a well-organized confined particle

Kurzbaum, Eyal; Raizner, Yasmin; Cohen, Oded; Suckeveriene, Ran Y.; Kulikov, Anatoly; Hakimi, Ben; Iasur-Kruh, Lilach; Armon, Robert; Farber, Yair; Menashe, Ofir

doi:10.1016/j.watres.2017.04.079

Cited by 66 publications

(24 citation statements)

References 40 publications

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“…Furthermore, the physical barrier prevents washout of the selective microorganisms from the bioreactor by a continuous outflow [20]. In our recent work on phenol biodegradation using a SBP-encapsulated Pseudomonas putida F1 strain, it was found that the encapsulated culture had a biodegradation rate similar to that of a bacterial suspension (not encapsulated) [21]. As far as we know, this capsule may present an efficient encapsulation matrix in terms of biodegradation rate, which is a significant advantage for OMP degradation in most WWTPs that operate using a short HRT (less than 24 h).…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of the Endocrine-Disrupting Chemical 17α-Ethynylestradiol (EE2) by Rhodococcus zopfii and Pseudomonas putida Encapsulated in Small Bioreactor Platform (SBP) Capsules

Menashe¹,

Raizner

Kuc

et al. 2020

Applied Sciences

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In this study, we present an innovative new bio-treatment approach for 17α-ethynyestradiol (EE2). Our solution for EE2 decontamination was accomplished by using the SBP (Small Bioreactor Platform) macro-encapsulation method for the encapsulation of two bacterial cultures, Rhodococcus zopfii (R. zopfii ) and Pseudomonas putida F1 (P. putida). Our results show that the encapsulated R. zopffi presented better biodegradation capabilities than P. putida F1. After 24 h of incubation on minimal medium supplemented with EE2 as a sole carbon source, EE2 biodegradation efficacy was 73.8% and 86.5% in the presence of encapsulated P. putida and R. zopfii, respectively. In the presence of additional carbon sources, EE2 biodegradation efficacy was 75% and 56.1% by R. zopfii and P. putida, respectively, indicating that the presence of other viable carbon sources might slightly reduce the EE2 biodegradation efficiency. Nevertheless, in domestic secondary effluents, EE2 biodegradation efficacy was similar to the minimal medium, indicating good adaptation of the encapsulated cultures to sanitary effluents and lack of a significant effect of the presence of other viable carbon sources on the EE2 biodegradation by the two encapsulated cultures. Our findings demonstrate that SBP-encapsulated R. zopfii and P. putida might present a practical treatment for steroidal hormones removal in wastewater treatment processes.

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Section: Introductionmentioning

confidence: 99%

Biodegradation of the Endocrine-Disrupting Chemical 17α-Ethynylestradiol (EE2) by Rhodococcus zopfii and Pseudomonas putida Encapsulated in Small Bioreactor Platform (SBP) Capsules

Menashe¹,

Raizner

Kuc

et al. 2020

Applied Sciences

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“…This unique membrane structure is assumed to allow molecule trafficking, not only through the membrane pores, but also across the entire capsule surface. As previously indicated by Kurzbaum et al [11], this might be the reason for the phenols' high trafficking rate and consequent rapid biodegradation rate. In the sterile adsorption experiment, the control system without SBP capsules showed no change in the EE2 concentration during the experimental interval.…”

Section: Biodegradation Of Ee2 By Sbp Capsulesmentioning

confidence: 63%

“…This unique membrane structure is assumed to allow molecule trafficking, not only through the membrane pores, but also across the entire capsule surface. As previously indicated by Kurzbaum et al [11], this might be the reason for the phenols' high trafficking rate and consequent rapid biodegradation rate. In other encapsulation techniques, the microbial culture is embedded in a polymer matrix such as sodium alginate and cellulose-based gel [23,24], Owing to the low availability of nutrients and oxygen due to poor diffusion rates, the bacteria show low biodegradation efficiency.…”

Section: Biodegradation Of Ee2 By Sbp Capsulesmentioning

confidence: 63%

“…Azaizeh et al [10] demonstrated the efficiency of an autochthonous bacterial consortium from olive mill wastewater in phenol biodegradation, while Kurzbaum et al [11] demonstrated phenol biodegradation using SBP-encapsulated Pseudomonas putida F1. The phenol-biodegradation rate of the suspended form and the SBP-encapsulated form were similar, demonstrating the potential of the SBP technology.…”

Section: Introductionmentioning

confidence: 99%

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LP-UV-Nano MgO2 Pretreated Catalysis Followed by Small Bioreactor Platform Capsules Treatment for Superior Kinetic Degradation Performance of 17α-Ethynylestradiol

et al. 2019

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A successful attempt to degrade synthetic estrogen 17α-ethynylestradiol (EE2) is demonstrated via combining photocatalysis employing magnesium peroxide (MgO2)/low-pressure ultraviolet (LP-UV) treatment followed by biological treatment using small bioreactor platform (SBP) capsules. Reusable MgO2 was synthesized through wet chemical synthesis and extensively characterized by X-ray diffraction (XRD) for phase confirmation, X-ray photoelectron spectroscopy (XPS) for elemental composition, Brunauer-Emmett-Teller (BET) to explain a specific surface area, scanning electron microscopy (SEM) imaging surface morphology, and UV-visible (Vis) spectrophotometry. The degradation mechanism of EE2 by MgO2/LP-UV consisted of LP-UV photolysis of H2O2 in situ (produced by the catalyst under ambient conditions) to generate hydroxyl radicals, and the degradation extent depended on both MgO2 and UV dose. Moreover, the catalyst was successfully reusable for the removal of EE2. Photocatalytic treatment by MgO2 alone required 60 min (~1700 mJ/cm2) to remove 99% of the EE2, whereas biodegradation by SBP capsules alone required 24 h to remove 86% of the EE2, and complete removal was not reached. The sequential treatment of photocatalysis and SBP biodegradation to achieve complete removal required only 25 min of UV (~700 mJ/cm2) and 4 h of biodegradation (instead of >24 h). The combination of UV photocatalysis and biodegradation produced a greater level of EE2 degradation at a lower LP-UV dose and at less biodegradation time than either treatment used separately, proving that synergetic photocatalysis and biodegradation are effective treatments for degrading EE2.

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“…For example, rhodococci completely degraded phenol at concentrations of 0.1-20.0 g/L within a duration range from 8 min to 8 days and removed 45%-100% of petroleum hydrocarbons at concentrations of 2%-30% (w/w) within 5 days to 14 months (see Table 1). Other referenced biocatalytic systems (based on Alcaligenes faecalis, Alcanivorax borkumensis, Bacillus cereus, Bacillus firmus, Halomonas hamiltonii, P. putida, Shewanalla chilikensis, and microbial fuel cells) provided complete phenol degradation at concentrations of 0.1-6.0 g/L within the time periods from 12 h to 8 days [138][139][140] and a 24%-90% removal of petroleum hydrocarbons at their concentrations of 1%-16% (w/w) in 3-66 days [141][142][143].…”

Section: Treatment Of Wastewater Containing Chlorophenolsmentioning

confidence: 99%

Advanced Rhodococcus Biocatalysts for Environmental Biotechnologies

2019

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The review is devoted to biocatalysts based on actinobacteria of the genus Rhodococcus, which are promising for environmental biotechnologies. In the review, biotechnological advantages of Rhodococcus bacteria are evaluated, approaches used to develop robust and efficient biocatalysts are discussed, and their relevant applications are given. We focus on Rhodococcus cell immobilization in detail (methods of immobilization, criteria for strains and carriers, and optimization of process parameters) as the most efficient approach for stabilizing biocatalysts. It is shown that advanced Rhodococcus biocatalysts with improved working characteristics, enhanced stress tolerance, high catalytic activities, human and environment friendly, and commercially viable are developed, which are suitable for wastewater treatment, bioremediation, and biofuel production.

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Encapsulated Pseudomonas putida for phenol biodegradation: Use of a structural membrane for construction of a well-organized confined particle

Cited by 66 publications

References 40 publications

Biodegradation of the Endocrine-Disrupting Chemical 17α-Ethynylestradiol (EE2) by Rhodococcus zopfii and Pseudomonas putida Encapsulated in Small Bioreactor Platform (SBP) Capsules

Biodegradation of the Endocrine-Disrupting Chemical 17α-Ethynylestradiol (EE2) by Rhodococcus zopfii and Pseudomonas putida Encapsulated in Small Bioreactor Platform (SBP) Capsules

LP-UV-Nano MgO2 Pretreated Catalysis Followed by Small Bioreactor Platform Capsules Treatment for Superior Kinetic Degradation Performance of 17α-Ethynylestradiol

Advanced Rhodococcus Biocatalysts for Environmental Biotechnologies

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