Enantioseparation of hydroxy acids on easy-to-prepare continuous beds for capillary electrochromatography

Schmid, Martin G.; Grobuschek, Nina; Lecnik, Oliver; Gübitz, Gerald; Végvári, Ákos; Hjertén, Stellan

doi:10.1002/1522-2683(200107)22:12<2616::aid-elps2616>3.0.co;2-g

Cited by 58 publications

(15 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This CSP was applied to the chiral separation of underivatized amino acids [537] and hydroxy acids [538] using phosphate buffer pH 4.4/copper(II)sulfate as mobile phase . The same capillary was used for CEC, capillary LC and pressure supported CEC.…”

Section: Monolithic Cspsmentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

Self Cite

225

135

View full text Add to dashboard Cite

This review gives a survey of different chiral separation principles and their use in high-performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), thin-layer chromatography (TLC), capillary electrophoresis (CE) and capillary electrochromatography (CEC) highlighting new developments and innovative techniques. The mechanisms of the different separation principles are briefly discussed and some selected applications are shown.

show abstract

Section: Monolithic Cspsmentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

Self Cite

225

135

View full text Add to dashboard Cite

show abstract

“…The applicability of this new approach to the chiral separation of underivatized AAs has been demonstrated. Additionally, this kind of solid phase in CEC easily prepared by in situ polymerization of monomers, including an L‐4‐hydroxyproline derivative showed chiral recognition ability for several hydroxy acids []. As a further study, by using diallyl‐dimethylammonium chloride (DADMAC) as a positive charge‐providing agent in CEC monolithic column, this phase was applied in the chiral separation of hydroxy monocarboxylic acids and hydroxy dicarboxylic acids, respectively [].…”

Section: Cle‐cecmentioning

confidence: 99%

Chiral separation using capillary electromigration techniques based on ligand exchange principle

Zhang

Mao

et al. 2012

J of Separation Science

View full text Add to dashboard Cite

Over the last couple of decades, researchers have developed diverse chiral separation methods emerged from a few chiral separation principles. This review article is primarily focused on the application of chiral ligand-exchange (CLE) principle in capillary electromigration techniques, such as capillary electrophoresis (CE) and capillary electrochromatography (CEC). First, the most commonly used CLE-CZE separation mode by using different kinds of central ions, such as Cu(II), Zn(II), borate ion, and other metal ions, has been introduced. Meanwhile, several kinds of surfactants have been applied as the micelle-forming agents in the CLE micellar electrokinetic chromatography mode. The highlight of recent research of CLE-CEC is the exploitation of novel columns for chiral separation. Then, two kinds of capillary columns, packed capillary and monolithic capillary column, have been briefly described. Finally, the effective application of these chiral separation methods has been presented, including the application in life science and food analysis area.

show abstract

“…Hjertén used a similar approach involving the copolymerization of methacrylamide, piperazine diacrylamide, and vinylsulfonic acid in the presence of a l-4-hydroxyproline derivative to afford monolithic CEC columns that could be used for ligand-exchange CEC of underivatized amino acids [102] or hydroxy acids [103]. Similar polymerizations in the presence of 2-hydroxy-3-allyloxy-propyl-␤-cyclodextrin led to monoliths that enabled enantiomeric resolution of acidic, neutral and basic drugs [104].…”

Section: Enantioselective Separationsmentioning

confidence: 99%