Emerging Lagochilascaris minor infections in domestic cats from Costa Rica: A zoonotic threat for the region

Solano-Barquero, Alberto; Estrada, Ana; Medaglia, Alejandro; Mon­te­n­e­gro, Víc­tor M.; Rojas, Alicia

doi:10.1016/j.vprsr.2022.100797

Cited by 2 publications

(4 citation statements)

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“…Therefore, this marker may resolve species at higher taxonomic levels [ 15 , 22 , 23 ], which may be useful for describing nematode communities in soil [ 24 , 25 ] or biological animal samples [ 26 ] using 18S-based metagenomics. However, it does not provide the necessary resolution for identifying unknown specimens in a clinical setting [ 27 ]. For instance, an unknown specimen may be erroneously classified if only an 18S fragment is analyzed, such as the case of L. minor from cats, which showed 99% homology to A. suum [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, it does not provide the necessary resolution for identifying unknown specimens in a clinical setting [ 27 ]. For instance, an unknown specimen may be erroneously classified if only an 18S fragment is analyzed, such as the case of L. minor from cats, which showed 99% homology to A. suum [ 27 ]. For these cases, the analysis of several and longer DNA fragments is recommended, together with the close morphological examination of specimens.…”

Section: Discussionmentioning

confidence: 99%

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Mitochondrial and ribosomal markers in the identification of nematodes of clinical and veterinary importance

Mejías-Alpízar,

Porras-Silesky,

Rodríguez

et al. 2024

Parasites Vectors

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Background Nematodes of the Ascarididae, Ancylostomatidae and Onchocercidae families are parasites of human and veterinary importance causing infections with high prevalence worldwide. Molecular tools have significantly improved the diagnosis of these helminthiases, but the selection of genetic markers for PCR or metabarcoding purposes is often challenging because of the resolution these may show. Methods Nuclear 18S rRNA, internal transcribed spacers 1 (ITS-1) and 2 (ITS-2), mitochondrial gene cytochrome oxidase 1 (cox1) and mitochondrial rRNA genes 12S and 16S loci were studied for 30 species of the mentioned families. Accordingly, their phylogenetic interspecies resolution, pairwise nucleotide p-distances and sequence availability in GenBank were analyzed. Results The 18S rRNA showed the least interspecies resolution since separate species of the Ascaris, Mansonella, Toxocara or Ancylostoma genus were intermixed in phylogenetic trees as opposed to the ITS-1, ITS-2, cox1, 12S and 16S loci. Moreover, pairwise nucleotide p-distances were significantly different in the 18S compared to the other loci, with an average of 99.1 ± 0.1%, 99.8 ± 0.1% and 98.8 ± 0.9% for the Ascarididae, Ancylostomatidae and Onchocercidae families, respectively. However, ITS-1 and ITS-2 average pairwise nucleotide p-distances in the three families ranged from 72.7% to 87.3%, and the cox1, 12S and 16S ranged from 86.4% to 90.4%. Additionally, 2491 cox1 sequences were retrieved from the 30 analyzed species in GenBank, whereas 212, 1082, 994, 428 and 143 sequences could be obtained from the 18S, ITS-1, ITS-2, 12S and 16S markers, respectively. Conclusions The use of the cox1 gene is recommended because of the high interspecies resolution and the large number of sequences available in databases. Importantly, confirmation of the identity of an unknown specimen should always be complemented with the careful morphological examination of worms and the analysis of other markers used for specific parasitic groups. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mitochondrial and ribosomal markers in the identification of nematodes of clinical and veterinary importance

Mejías-Alpízar,

Porras-Silesky,

Rodríguez

et al. 2024

Parasites Vectors

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“…It is a silent and chronic disease, and over the course of years the symptoms may be hidden. The clinical picture of the disease presents itself until the parasite invades the subcutaneous tissues, central nervous system and bone tissues (Solano-Barquero et al 2022). L. minor is mainly reported in wild felines, with rare reports in domestic felines.…”

Section: Lagochilascariasismentioning

confidence: 99%

“…2). Although lagochilarscariasis is an uncommon parasitic disease in humans, people must be cautious on probable outbreaks due to the climate change (Quintana de Moura et al 2012;Solano-Barquero et al 2022).…”

Section: Lagochilascariasismentioning

confidence: 99%

Zoonoses Associated with Geohelminthiases

2023

IJAB

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This chapter presents a general and updated overview of the different zoonoses associated with soiltransmitted helminth infections. The topics presented in the chapter are: Angiostrongyliasis; Ascariasis; Capillariasis; Lagochilascariasis; Mammomonogamiasis; Strongyloidiasis; Cutaneous larva migrans. In this context, zoonotic diseases are a major public health problem because animals are the source of human pathogens. The emergence and re-emergence of zoonotic diseases have been attributed to a number of anthropogenic and natural factors, including vector biology, urbanization, climate change, animal migration and trade, travel and tourism, among others. Animals are the origin of a large number of infectious diseases that affect humans. About 60% of newly discovered human infections are zoonotic. Because of this, it is important to consider the areas where we live and the risks of latent diseases in the regions. In addition, it is extremely important to give importance to common wound accidents, because it is better to prevent silent diseases that can be hidden for years through good food and wound asepsis and go to the doctor.

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Emerging Lagochilascaris minor infections in domestic cats from Costa Rica: A zoonotic threat for the region

Cited by 2 publications

References 36 publications

Mitochondrial and ribosomal markers in the identification of nematodes of clinical and veterinary importance

Mitochondrial and ribosomal markers in the identification of nematodes of clinical and veterinary importance

Zoonoses Associated with Geohelminthiases

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