Emerging features of ER resident J-proteins in plants

Ohta, Masaru; Takaiwa, Fumio

doi:10.4161/psb.28194

Cited by 28 publications

(25 citation statements)

References 47 publications

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“…It was reported that ERDJ3A is a co-chaperone with heat shock proteins (HSPs) expressed in plant cells. The gene could be greatly induced under ER stress in rice and facilitates the delivery of unfolded proteins between vacuoles and ER 25 . We found its expression was elevated 5-8 times in QPM over o2, and that a 26,022-bp retrotransposon was located downstream of ERDJ3A DNA sequences ( Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The 43 of 49 increased HSPs indicates stronger UPRs in QPM. In rice, ERdj3A, which is located in protein storage vacuoles and induced under ER stress, is involved in protein folding and plant immunity 25 . An elevated ERDJ3A in QPM could reflect physiological stress created by refolding proteins or facilitating ER-associated protein degradation.…”

Section: Discussionmentioning

confidence: 99%

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Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize

Xiang

Huang

et al. 2020

Nat Commun

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Mutation of o2 doubles maize endosperm lysine content, but it causes an inferior kernel phenotype. Developing quality protein maize (QPM) by introgressing o2 modifiers (Mo2s) into the o2 mutant benefits millions of people in developing countries where maize is a primary protein source. Here, we report genome sequence and annotation of a South African QPM line K0326Y, which is assembled from single-molecule, real-time shotgun sequencing reads collinear with an optical map. We achieve a N50 contig length of 7.7 million bases (Mb) directly from long-read assembly, compared to those of 1.04 Mb for B73 and 1.48 Mb for Mo17. To characterize Mo2s, we map QTLs to chromosomes 1, 6, 7, and 9 using an F 2 population derived from crossing K0326Y and W64Ao2. RNA-seq analysis of QPM and o2 endosperms reveals a group of differentially expressed genes that coincide with Mo2 QTLs, suggesting a potential role in vitreous endosperm formation.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize

Xiang

Huang

et al. 2020

Nat Commun

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“…Knockdown mutants of SHD have defects in meristem size control, especially at elevated ambient growth temperature (Ishiguro et al ., ). ER‐localized DNAJ domain‐containing proteins (ERdj proteins) are BiP co‐chaperones that regulate the chaperoning activities of their cognate BiPs (Ohta & Takaiwa, ). Mutations in Arabidopsis ERdj2A result in pollen germination defects (Yamamoto et al ., ).…”

Section: Protein Folding and Er‐associated Protein Degradationmentioning

confidence: 99%

Managing the protein folding demands in the endoplasmic reticulum of plants

2016

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Summary Endoplasmic reticulum (ER) stress occurs in plants during certain developmental stages or under adverse environmental conditions, as a result of the accumulation of unfolded or misfolded proteins in the ER. To minimize the accumulation of misfolded proteins in the ER, a protein quality control (PQC) system monitors protein folding and eliminates misfolded proteins through either ER‐associated protein degradation (ERAD) or autophagy. ER stress elicits the unfolded protein response (UPR), which enhances the operation in plant cells of the ER protein folding machinery and the PQC system. The UPR also reduces protein folding demands in the ER by degrading mRNAs encoding secretory proteins. In plants subjected to severe or chronic stress, UPR promotes programmed cell death (PCD). Progress in the field in recent years has provided insights into the regulatory networks and signaling mechanisms of the ER stress responses in plants. In addition, novel physiological functions of the ER stress responses in plants for coordinating plant growth and development with changing environment have been recently revealed.

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“…BiPs and ERdjs are essential for plant development and stress response by mediating the ERQC equilibrium and PCD execution (Reis et al, 2011;Ohta & Takaiwa, 2014). For example, BiPs participate in senescence, seed development, and hypersensitive response by regulating PCD and ERQC equilibrium (Yasuda et al, 2009;Wakasa et al, 2011;Carvalho et al, 2014).…”

Section: Researchmentioning

confidence: 99%

Excessive UDPG resulting from the mutation of UAP1 causes programmed cell death by triggering reactive oxygen species accumulation and caspase‐like activity in rice

Xiao

Zhou

et al. 2017

New Phytologist

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Lesion mimic mutants are valuable to unravel the mechanisms governing the programmed cell death (PCD) process. Uridine 5'-diphosphoglucose-glucose (UDPG) functions as a signaling molecule activating multiple pathways in animals, but little is known about its function in plants. Two novel allelic mutants of spl29 with typical PCD characters and reduced pollen viability were obtained by ethane methyl sulfonate mutagenesis in rice cv Kitaake. The enzymatic analyses showed that UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) irreversibly catalyzed the decomposition of UDPG. Its activity was severely destroyed and caused excessive UDPG accumulation, with the lesion occurrence associated with the enhanced caspase-like activities in spl29-2. At the transcriptional level, several key genes involved in endoplasmic reticulum stress and the unfolded protein response were abnormally expressed. Moreover, exogenous UDPG could aggravate lesion initiation and development in spl29-2. Importantly, exogenous UDPG and its derivative UDP-N-acetylglucosamine could induce reactive oxygen species (ROS) accumulation and lesion mimics in Kitaake seedlings. These results suggest that the excessive accumulation of UDPG, caused by the mutation of UAP1, was a key biochemical event resulting in the lesion mimics in spl29-2. Thus, our findings revealed that UDPG might be an important component involved in ROS accumulation, PCD execution and lesion mimicking in rice, which also provided new clues for investigating the connection between sugar metabolism and PCD process.

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Emerging features of ER resident J-proteins in plants

Cited by 28 publications

References 47 publications

Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize

Long-read sequencing reveals genomic structural variations that underlie creation of quality protein maize

Managing the protein folding demands in the endoplasmic reticulum of plants

Excessive UDPG resulting from the mutation of UAP1 causes programmed cell death by triggering reactive oxygen species accumulation and caspase‐like activity in rice

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