Emerging and Dynamic Biomedical Uses of Ferritin

Chiou, Brian; Connor, James R.

doi:10.3390/ph11040124

Cited by 84 publications

(83 citation statements)

References 137 publications

(181 reference statements)

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“…The ferritin was genetically engineered by inserting nanobodies sequence at the C-terminus, thereby replacing the ε-helix of the P. furiosus ferritin subunit. Then, the ferritin fused nanobody (fenobody) can self-assemble into a nanocage and more nanobodies are displayed on the surface of nanocage [24,44]. Then, the fenobody showed higher affinity to antigen compared with the traditional nanobody.…”

Section: Discussionmentioning

confidence: 99%

Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus

Zhu

et al. 2020

J Nanobiotechnol

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Background: Traditional sandwich enzyme-linked immunosorbent assay (ELISA) using polyclonal and monoclonal antibodies as reagents presents several drawbacks, including limited amounts, difficulty in permanent storage, and required use of a secondary antibody. Nanobodies can be easily expressed with different systems and fused with several tags in their tertiary structure by recombinant technology, thus offering an effective detection method for diagnostic purposes. Recently, the fenobody (ferritin-fused nanobody) and RANbody (nanobody-fused reporter) have been designed and derived from the nanobody for developing the diagnostic immunoassays. However, there was no report about developing the sandwich ELISA using the fenobody and RANbody as pairing reagents. Results: A platform for developing a sandwich ELISA utilizing fenobody as the capture antibody and RANbody as the detection antibody was firstly designed in the study. Newcastle disease virus (NDV) was selected as the antigen, from which 13 NDV-specific nanobodies were screened from an immunized Bactrian camel. Then, 5 nanobodies were selected to produce fenobodies and RANbodies. The best pairing of fenobodies (NDV-fenobody-4, 800 ng/well) and RANbodies (NDV-RANbody-49, 1:10) was determined to develop the sandwich ELISA for detecting NDV. The detection limits of the assay were determined to be 2 2 of hemagglutination (HA) titers and 10 ng of purified NDV particles. Compared with two commercial assays, the developed assay shows higher sensitivity and specificity. Meanwhile, it exhibits 98.7% agreement with the HA test and can detect the reference NDV strains belonging to Class II but not Class I. Conclusions: In the presented study, the 13 anti-NDV nanobodies binding the NDV particles were first produced. Then, for the first time, the sandwich ELISA to detect the NDV in the different samples has been developed using the fenobody and RANbody as reagents derived from the nanobodies. Considering the rapidly increasing generation of nanobodies, the platform can reduce the cost of production for the sandwich ELISA and be universally used to develop assays for detecting other antigens.

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Section: Discussionmentioning

confidence: 99%

Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus

Zhu

et al. 2020

J Nanobiotechnol

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“…Ferritin heavy chain protein has found many biological applications in nanomedicines and molecular diagnostics [15][16][17]. Lately, a few constructs based on ferritin have been developed as antivirus and anticancer vaccines [10,11,13].…”

Section: Construction Of Rbm-hftnmentioning

confidence: 99%

Human H-ferritin presenting RBM of spike glycoprotein as potential vaccine of SARS-CoV-2

Yao

Lao

Liu

et al. 2020

Preprint

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The outbreak of COVID-19 has so far inflicted millions of people all around the world and will have a long lasting effect on every aspect of everyone's life. Yet there is no effective approved treatment for the disease. In an effort of utilizing human ferritin as nanoplatform for drug delivery, we engineered a fusion protein by presenting receptor-binding motif (RBM) of SARS-CoV-2 virus spike glycoprotein on the N-terminus of ferritin subunits. The designed fusion protein with a cage-like structure, similar to that of corona virus, is a potential anti-SARS-CoV-2 vaccine. We hereby show the construction, preparation, and characterization of the fusion protein RBM-HFtn. Our initial affinity study confirmed its biological activity towards ACE2 receptor which suggests its mode of action against SARS-CoV-2 could be either through vaccine therapy or blocking the cellular entry of virus as antagonist of ACE2 receptor.

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“…However, in addition to iron, ferritin has the unique ability to encapsulate and deliver other molecules. Many recent reviews have focused on various aspects of the use of this nanocage as a drug delivery system [9,10]. This review aims to discuss the peculiar role of an apoferritin cage not only for the specific delivery of MRI CAs but also to obtain significant relaxivity enhancement with consequent efficacy improvement.…”

Section: Introductionmentioning

confidence: 99%

“…These peptide chains are present in different ratios in various organs to form a cage architecture of 12 nm in external diameter with an interior cavity of 8 nm [11]. Although functions of ferritin are traditionally associated with intracellular iron storage, recent studies have demonstrated ferritin ability for iron delivery and transport [9]. In particular, studies have demonstrated H-ferritin-mediated iron delivery to the brain through the blood-brain barrier, demonstrating that H-ferritin can replace transferrin as an iron source for oligodendrocytes in vitro [12].…”

Section: Introductionmentioning

confidence: 99%

Ferritin: A Platform for MRI Contrast Agents Delivery

et al. 2019

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The search for high relaxivities and increased specificity continues to be central to the development of paramagnetic contrast agents for magnetic resonance imaging (MRI). Ferritin, due to its unique surface properties, architecture, and biocompatibility, has emerged as a natural nanocage that can potentially help to reach both these goals. This review aims to highlight recent advances in the use of ferritin as a nanoplatform for the delivery of metal-based MRI contrast agents (containing Gd3+, Mn2+, or Fe2O3) alone or in combination with active molecules used for therapeutic purposes. The collected results unequivocally show that the use of ferritin for contrast agent delivery leads to more accurate imaging of cancer cells and a significantly improved targeted therapy.

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Emerging and Dynamic Biomedical Uses of Ferritin

Cited by 84 publications

References 137 publications

Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus

Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus

Human H-ferritin presenting RBM of spike glycoprotein as potential vaccine of SARS-CoV-2

Ferritin: A Platform for MRI Contrast Agents Delivery

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