Emergence of Tilapia Lake Virus associated with mortalities of farmed Nile Tilapia Oreochromis niloticus (Linnaeus 1758) in India

Behera, Bijay Kumar; Pradhan, Pravata Kumar; Swaminathan, T. Raja; Sood, Neeraj; Paria, Prasenjit; Das, A. K.; Verma, Dev Kumar; Kumar, Raj; Yadav, Manoj Kumar; Dev, A.K.; Parida, Pranaya Kumar; Das, Basanta Kumar; Lal, Kuldeep Kumar; Jena, J. K.

doi:10.1016/j.aquaculture.2017.11.025

Cited by 188 publications

(169 citation statements)

References 19 publications

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“…In all the trials, the TiLV infection was reproduced in the experimentally challenged tilapia species. The mortality pattern of TiLV‐infected fish observed in the present study was found to be the same as reported by Tattiyapong et al () and Behera et al (). Many field samples showed positive to TiLV by RT‐PCR, but it is essential to reproduce the disease and re‐isolate the causative agent from challenged fish to confirm the aetiology of disease.…”

Section: Discussionsupporting

confidence: 91%

“…Previous studies revealed that the TiLV was found to be a serious viral pathogen and very contagious in Nile and red tilapia, making it a serious threat to tilapia culture worldwide. Previous studies also revealed that Nile and red tilapia are highly susceptible to TiLV (Amal et al, ; Behera et al, ; Eyngor et al, ; Tattiyapong et al, ). Hence, it is important to investigate TiLV infection in other fish species and to identify the natural potential carriers or reservoirs for this killer virus.…”

Section: Discussionmentioning

confidence: 89%

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In vitro propagation of tilapia lake virus in cell lines developed from Oreochromis mossambicus

Nanthini¹,

Majeed²,

Vimal³

et al. 2019

Journal of Fish Diseases

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Tilapia lake virus (TiLV)‐suspected samples of tilapia were collected from grow‐out ponds located with clinical signs and mortality ranged from 5% to 50%. The reverse transcription–polymerase chain reaction (RT‐PCR) assay revealed the presence of TiLV in the disease outbreak ponds. Cell lines were developed from heart, gill and eye of Mozambique tilapia and characterized. Morphologically, these cell lines are composed of epithelioid cells. The optimum growth of these cells was observed at 28°C and 20% concentration of FBS. After cryopreservation, 70%–90% of cells were found to be viable. The cells of all three cell lines were found to be positive to fibronectin and pancytokeratin. PCR amplification of 16S rRNA and COI of O. mossambicus confirmed the origin of these cell lines from O. mossambicus. Heart and gill cell lines were found to be highly susceptible to TiLV and found to be useful for its isolation from infected fish samples. The experimental infection was carried out in O. niloticus and O. mossambicus using the TiLV propagated in susceptible cell lines. The RT‐PCR results revealed the presence of TiLV in brain, gill, liver, kidney, spleen, eye, muscle, intestine and heart of experimentally infected O. niloticus and O. mossambicus.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 89%

In vitro propagation of tilapia lake virus in cell lines developed from Oreochromis mossambicus

Nanthini¹,

Majeed²,

Vimal³

et al. 2019

Journal of Fish Diseases

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“…At present, TiLV has been reported in several Asian countries. Several publications and notifications to the OIE have been made by the Chinese Taipei (OIE, ), India (Behera et al., ), Israel (OIE, ), Thailand (OIE, ), Malaysia (Amal et al., ; OIE, ) and the Philippines (OIE, ). Other than the above countries, Ecuador, Colombia and Egypt have also been reported to be affected by TiLV (Fathi et al., ; Ferguson et al., ; Tsofack et al., ).…”

Section: Summary Of Tests Results Conducted On the Tilapia And River mentioning

confidence: 99%

First detection of tilapia lake virus (TiLV) in wild river carp (Barbonymus schwanenfeldii) at Timah Tasoh Lake, Malaysia

Abdullah¹,

Ramly²,

Ridzwan³

et al. 2018

Journal of Fish Diseases

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“…Tilapia lake virus (TiLV) was first identified in wild and farm‐raised tilapia showing clinical signs of skin discoloration, ocular alterations and lethargy in Israel (Eyngor et al., ). Subsequently, TiLV was found in many countries including Ecuador (Bacharach et al., ), Colombia (Kembou Tsofack et al., ), Egypt (Fathi et al., ; Nicholson et al., ), Thailand (Dong et al., ; Surachetpong et al., ), India (Behera et al., ), Indonesia (Koesharyani, Gardenia, Widowati, Khumaira, & Rustianti, ), Uganda and Tanzania (Mugimba et al., ), and Malaysia (Amal et al., ). In clinically infected fish, TiLV has been detected in several organs including liver, spleen, anterior kidney, gills, heart as well as muscle (Dong et al., ; Tattiyapong, Dachavichitlead, & Surachetpong, ; Tattiyapong, Sirikanchana, & Surachetpong, ).…”

Section: Introductionmentioning

confidence: 99%

“…In clinically infected fish, TiLV has been detected in several organs including liver, spleen, anterior kidney, gills, heart as well as muscle (Dong et al., ; Tattiyapong, Dachavichitlead, & Surachetpong, ; Tattiyapong, Sirikanchana, & Surachetpong, ). To date, various methods have been used for the diagnosis of TiLV such as reverse transcription–polymerase chain reaction (RT‐PCR), RT‐quantitative PCR (RT‐qPCR), histopathology and viral isolation in susceptible cells (Behera et al., ; Jaemwimol et al., ; Kembou Tsofack et al., ; Tattiyapong et al., ; Waiyamitra et al., ).…”

Section: Introductionmentioning

confidence: 99%

Minimal risk of tilapia lake virus transmission via frozen tilapia fillets

Thammatorn

Rawiwan

Surachetpong

2018

Journal of Fish Diseases

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Recent outbreaks of a novel tilapia lake virus (TiLV) have raised concerns regarding the international spread of TiLV in frozen tilapia products. This study investigated the potential risks of frozen tilapia fillet as a source of TiLV transmission. It revealed that TiLV genomic RNA could be detected in tilapia fillet and the virus isolated from non‐frozen and frozen fillets with clinical TiLV infection stored up to 28 days caused a cytopathic effect (CPE) formation in the susceptible cell line in vitro. However, frozen fillets from clinical TiLV infection stored for 90 and 120 days did not cause CPE in the susceptible cell line. Similarly, CPE was not observed in TiLV isolated from subclinically TiLV‐infected fish fillets. In addition, in vivo bioassay revealed that despite the presence of TiLV isolated from subclinically TiLV‐infected fillet stored at −20°C for 14 days, there was no evidence of TiLV disease in naïve red hybrid tilapia based on the absence of clinical signs and mortality and without the detection of TiLV genomic RNA using reverse transcription–quantitative polymerase chain reaction assay. Collectively, these findings suggested minimal risk of transmission of TiLV via frozen tilapia fillets.

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Emergence of Tilapia Lake Virus associated with mortalities of farmed Nile Tilapia Oreochromis niloticus (Linnaeus 1758) in India

Cited by 188 publications

References 19 publications

In vitro propagation of tilapia lake virus in cell lines developed from Oreochromis mossambicus

In vitro propagation of tilapia lake virus in cell lines developed from Oreochromis mossambicus

First detection of tilapia lake virus (TiLV) in wild river carp (Barbonymus schwanenfeldii) at Timah Tasoh Lake, Malaysia

Minimal risk of tilapia lake virus transmission via frozen tilapia fillets

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