Emergence ofKlebsiella pneumoniaeST273 CarryingblaNDM-7and ST656 CarryingblaNDM-1in Manila, Philippines

Chou, Andrew; Roa, Marylette B.; Evangelista, Michael A.; Sulit, Arielle Kae; Lagamayo, Evelina; Torres, Brian Carlmichael; Klinzing, David C.; Daroy, Maria Luisa G; Navoa-Ng, Josephine Anne; Sucgang, Richard; Zechiedrich, Lynn

doi:10.1089/mdr.2015.0205

Cited by 29 publications

(16 citation statements)

References 26 publications

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“…We found that CPE isolates that are often found in clinical settings were also present in the environment. KPC, GES, and OXA-48 producers, which have not yet been detected in clinical settings in the Philippines (14,15,17), were detected in both hospital sewage and river water. Although it is unclear whether these CPE isolates originated from humans or the natural environment, it is possible that they may spread from the natural environment to clinical settings in the future.…”

Section: Resultsmentioning

confidence: 94%

Environmental Presence and Genetic Characteristics of Carbapenemase-Producing Enterobacteriaceae from Hospital Sewage and River Water in the Philippines

Suzuki

Nazareno

Nakano

et al. 2020

Appl Environ Microbiol

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This study aimed to evaluate the prevalence and genetic characteristics of carbapenemase-producing Enterobacteriaceae (CPE) in hospital sewage and river water in the Philippines, which has a typical tropical maritime climate. We collected 83 water samples from 7 hospital sewage and 10 river water sites. CPE were identified using CHROMagar mSuperCARBA, and Gram-negative strains were identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) or 16S rRNA gene sequencing. Resistance genes in Enterobacteriaceae strains were identified using PCR and DNA sequencing, and transferability of carbapenemase genes from the CPE was investigated with conjugation experiments. Genotyping was performed using multilocus sequence typing (MLST) for Escherichia coli and Klebsiella pneumoniae. Out of 124 Enterobacteriaceae isolates, we identified 51 strains as CPE and divided these into 7 species, 11 E. coli, 14 Klebsiella spp., 15 Enterobacter spp., and 11 others, including 4 additional species. Conjugation experiments via broth mating and using E. coli J53 revealed that 24 isolates can transfer carbapenemase-encoding plasmids. MLST analysis showed that 6 of 11 E. coli isolates belonged to clonal complex 10 (CC10). Of 11 K. pneumoniae strains, 9 unique sequence types (STs) were identified, including ST147. Five types of carbapenemase genes were identified, with the most prevalent being NDM (n = 39), which is epidemic in clinical settings in the Philippines. E. coli CC10 and K. pneumoniae ST147, which are often detected in clinical settings, were the dominant strains. In summary, our results indicate that hospital sewage and river water are contaminated by CPE strains belonging to clinically important clonal groups. IMPORTANCE Carbapenemase-producing Enterobacteriaceae (CPE) cause severe health care-associated infections, and their increasing prevalence is a serious concern. Recently, natural ecosystems have been recognized as important reservoirs of antibiotic resistance genes. We investigated the prevalence and genetic characteristics of CPE isolated from the environment (hospital sewage and river water) in the Philippines and found several CPE, including Escherichia coli and other species, with different carbapenemases. The most prevalent carbapenemase gene type was NDM, which is endemic in clinical settings. This study revealed that isolates belonging to carbapenemase-producing E. coli CC10 and K. pneumoniae sequence type 147 (ST147), which are often detected in clinical settings, were dominant in the natural environment. Our work here provides a report on the presence and characteristics of CPE in the environment in the Philippines and demonstrates that both hospital sewage and river water are contaminated by CPE strains belonging to clinically important clonal groups.

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Section: Resultsmentioning

confidence: 94%

Environmental Presence and Genetic Characteristics of Carbapenemase-Producing Enterobacteriaceae from Hospital Sewage and River Water in the Philippines

Suzuki

Nazareno

Nakano

et al. 2020

Appl Environ Microbiol

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“…These different standard sequences have also been obtained elsewhere in the world. The type 273 sequence was found in a strain of K. pneumoniae, isolated from a patient with pneumonia in the Philippines, which carried the blaNDM-7 gene [22]. K. pneumoniae ST 273 has been reported in Italy, Norway, Russia and the United Kingdom.…”

Section: Discussionmentioning

confidence: 98%

Molecular Detection of the Arr-2 Gene in Escherichia coli and Klebsiella pneumoniae Resistant to Rifampicin in Abidjan, Côte D'Ivoire

Victoire

Guessennd

Toty

et al. 2018

MRJI

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“…These isolates corresponded to ST656 and ST392, which have not been previously described in Mexico but which have been described in nosocomial infections in other regions of world. K. pneumoniae ST665 has been described in China and Philippines [31] , [32] as carrying ESBL and carbapenemase genes respectively. In the case of K. pneumoniae , ST392 harbouring KPC-3 and CTX-M alleles has been documented in the United States [33] , [34] .…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections

Garza–Ramos

Barrios-Camacho

Moreno-Dominguez

et al. 2018

New Microbes and New Infections

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Klebsiella spp. isolates from community-acquired infections were characterized. A total of 39 Klebsiella spp. isolates were obtained from outpatients at four rural hospitals in Mexico (2013–2014). The biochemical tests identified all as being K. pneumoniae. The molecular multiplex-PCR test identified 36 (92.4%) K. pneumoniae isolates and one (2.5%) K. variicola isolate, and phylogenetic analysis of the rpoB gene identified two isolates (5.1%) belonging to K. quasipneumoniae subsp. quasipneumoniae and K. quasivariicola. The last one was confirmed by phylogenetic analysis of six-loci concatenated genes. Mostly the isolates were multidrug resistant; however, a minority were extended-spectrum β-lactamase producing (10.2%). The extended-spectrum β-lactamase CTX-M-15 gene was identified in these isolates. Analysis of biofilm production and the hypermucoviscosity phenotype showed a total of 35 (92.3%) and seven (17.9%) of the isolates were positive for these phenotypes respectively. The K2 (4/39, 10.2%), K5 (2/39, 5.1%) and K54 (1/39, 2.5%) serotypes were identified in seven (17.9%) of the isolates, and only 28.5% (2/7) hypermucoviscous isolates were positive for the K2 and K5 serotypes. In general, the sequence type (ST) analysis and phylogenetic analysis of seven multilocus sequence typing loci were heterogeneous; however, ST29 was the most prevalent ST in the analysed isolates, accounting for 19% (4/21) of the total isolates. Two of the four ST29 isolates had the hypermucoviscosity phenotype. The virulence factors for fimbriae were the most prevalent, followed by siderophores. Community-acquired infections are caused by various species from Klebsiella genus, with different profiles of antibiotic resistance and heterogeneous virulence factors.

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Emergence ofKlebsiella pneumoniaeST273 Carryingbla_NDM-7and ST656 Carryingbla_NDM-1in Manila, Philippines

Cited by 29 publications

References 26 publications

Environmental Presence and Genetic Characteristics of Carbapenemase-Producing Enterobacteriaceae from Hospital Sewage and River Water in the Philippines

Environmental Presence and Genetic Characteristics of Carbapenemase-Producing Enterobacteriaceae from Hospital Sewage and River Water in the Philippines

Molecular Detection of the Arr-2 Gene in Escherichia coli and Klebsiella pneumoniae Resistant to Rifampicin in Abidjan, Côte D'Ivoire

Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections

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