EMCD, a hypoglycemic triterpene isolated from Momordica charantia wild variant, attenuates TNF-α-induced inflammation in FL83B cells in an AMP-activated protein kinase-independent manner

Cheng, Hsueh‐Ling; Kuo, Ching-Yi; Liao, Yun‐Wen; Lin, Chen-Chen

doi:10.1016/j.ejphar.2012.05.033

Cited by 54 publications

(43 citation statements)

References 27 publications

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“…TNF-α has been demonstrated to enhance the expression of PTP-1B in FL83B cells (Cheng et al, 2012). Some natural products were shown to inhibit the activity of PTP-1B (Popov, 2011), and PTP-1B inhibitors were reported to have hypoglycaemic effects in diabetic animal models (Jiang, Guo, Shi, Guo, & Wang, 2013).…”

Section: Mechanisms Underlying the Actions Of Dhm And Thcmentioning

confidence: 99%

“…The supernatant was collected and the protein concentration was analyzed using Bradford assay reagent (Bio-Rad, Hercules, CA, USA). Equal amounts of proteins were sampled and subjected to electrophoresis and Western blotting as described previously (Cheng, Chang, Cheng, Liu, & Chen, 2006;Cheng, Kuo, Liao, & Lin, 2012). The resulting X-ray films were scanned and band intensities were analyzed using the software of an imaging system (UVP BioSpectrum, UVP, LLC, Upland, CA, USA).…”

Section: Western Blot Analysismentioning

confidence: 99%

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Bitter melon triterpenes work as insulin sensitizers and insulin substitutes in insulin-resistant cells

Chang

Chou

Liao

et al. 2015

Journal of Functional Foods

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Section: Mechanisms Underlying the Actions Of Dhm And Thcmentioning

confidence: 99%

Section: Western Blot Analysismentioning

confidence: 99%

Bitter melon triterpenes work as insulin sensitizers and insulin substitutes in insulin-resistant cells

Chang

Chou

Liao

et al. 2015

Journal of Functional Foods

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“…Some pharmacological and safety studies of this herb have been carried out 16,17 . In addition to hypoglycaemic activity, M.charantia has been shown to have antioxidant [18][19][20] , anti-tumour [21][22][23][24][25] , neuroprotective 26 , anti-inflammatory [27][28][29] and antimicrobial activity 30,31 . It has a resistance modifying effect for aminoglycosides against methicillin-resistant Staphylococcus aureus 32 .…”

Section: Introductionmentioning

confidence: 99%

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

Nkambo¹,

Anyama²,

Onegi³

2014

Afr H. Sci.

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Background: Momordica charantia L. is a medicinal plant commonly used in the management of diabetes mellitus. Objectives: We investigated the blood glucose lowering effect of the methanolic fruit extract of the Ugandan variety of M. charantia L. in alloxan-induced diabetic albino rats. Methods: 500g of M. charantia powder were macerated in methanol and the extract administered to two groups of alloxaninduced diabetic rats. The first group received 125mg/kg, the second 375mg/kg and a third group 7mg/ kg of metformin. A fourth group received 1ml normal saline. Fasting blood glucose (FBG) levels were measured at 0.5,1,2,3,5,8 and 12 hours and compared using one-way ANOVA. Results: There was an initial rise in FBG for 1 hour after administration of extracts followed by steep reductions. Significant reduction in FBG occurred at 2 hours for 125mg/kg of extract (-3.2%, 313±25.9 to 303±25.0mg/dL, p = 0.049), 375mg/ kg of extract (-3.9%, 356±19.7 to 342±20.3mg/dL, p = 0.001), and metformin (-2.6%, 344±21.7 to 335±21.1mg/dL, p = 0.003) when compared to normal saline. The maximum percentage reduction in FBG by both extracts occurred between 3 and 12 hours post dose. Conclusions: The methanolic fruit extract of M. charantia exhibits dose dependent hypoglycaemic activity in vivo.

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“…The supernatant was collected and the protein concentration analysed using Bradford assay reagent (Bio-Rad, Hercules, CA, USA). Equal amounts of proteins were sampled and subjected to electrophoresis and Western blotting as described previously (Cheng, Kuo, Liao, & Lin, 2012). Alternatively, immunoreactive bands on the PVDF membrane (Millipore, Bedford, MA, USA) were detected using an imaging system (UVP BioSpectrum, UVP, LLC, Upland, CA, USA) and band intensities analysed by the supplied software.…”

Section: Western Blot Analysismentioning

confidence: 99%

Constituents of the stem of Cucurbita moschata exhibit antidiabetic activities through multiple mechanisms

Chang

Hsu

et al. 2014

Journal of Functional Foods

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EMCD, a hypoglycemic triterpene isolated from Momordica charantia wild variant, attenuates TNF-α-induced inflammation in FL83B cells in an AMP-activated protein kinase-independent manner

Cited by 54 publications

References 27 publications

Bitter melon triterpenes work as insulin sensitizers and insulin substitutes in insulin-resistant cells

Bitter melon triterpenes work as insulin sensitizers and insulin substitutes in insulin-resistant cells

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

Constituents of the stem of Cucurbita moschata exhibit antidiabetic activities through multiple mechanisms

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