Embedding objects during 3D printing to add new functionalities

Yuen, Po Ki

doi:10.1063/1.4958909

Cited by 44 publications

(25 citation statements)

References 29 publications

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“…Future improvement in the SLA printing technology may allow for similar functional integration achievable in FDM-printed microfluidics, where functional parts (e.g. a cover slide) can be incorporated during the printing process [43] to realize a uni-body device with an imaging port.…”

Section: Discussionmentioning

confidence: 99%

A 3D printed microfluidic perfusion device for multicellular spheroid cultures

et al. 2017

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The advent of 3D printing technologies promises to make microfluidic organ-on-chip technologies more accessible for the biological research community. To date, hydrogel-encapsulated cells have been successfully incorporated into 3D printed microfluidic devices. However, there is currently no 3D printed microfluidic device that can support multicellular spheroid culture, which facilitates extensive cell–cell contacts important for recapitulating many multicellular functional biological structures. Here, we report a first instance of fabricating a 3D printed microfluidic cell culture device capable of directly immobilizing and maintaining the viability and functionality of 3D multicellular spheroids. We evaluated the feasibility of two common 3D printing technologies i.e. stereolithography (SLA) and PolyJet printing, and found that SLA could prototype a device comprising of cell immobilizing micro-structures that were housed within a microfluidic network with higher fidelity. We have also implemented a pump-free perfusion system, relying on gravity-driven flow to perform medium perfusion in order to reduce the complexity and footprint of the device setup, thereby improving its adaptability into a standard biological laboratory. Finally, we demonstrated the biological performance of the 3D printed device by performing pump-free perfusion cultures of patient-derived parental and metastatic oral squamous cell carcinoma tumor and liver cell (HepG2) spheroids with good cell viability and functionality. This paper presents a proof-of-concept in simplifying and integrating the prototyping and operation of a microfluidic spheroid culture device, which will facilitate its applications in various drug efficacy, metabolism and toxicity studies.

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Section: Discussionmentioning

confidence: 99%

A 3D printed microfluidic perfusion device for multicellular spheroid cultures

et al. 2017

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“…Separation membranes have also been integrated with 3D printing indirectly, either in the mid‐print, or postprint. Yuen used FDM for the fabrication of various fluidic devices with integrated porous membranes, or light‐diffusing fibers [39]. The bottom half of the fluidic device with open serpentine channels was first printed, then the printing was paused, and a porous commercially available membrane was inserted, and the printing was resumed to finish the top half of the fluid device.…”

Section: D Printed Separation Membranesmentioning

confidence: 99%

3D Printing in analytical sample preparation

Ceballos

Balavandy

et al. 2020

J of Separation Science

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In the last 5 years, additive manufacturing (three-dimensional printing) has emerged as a highly valuable technology to advance the field of analytical sample preparation. Three-dimensional printing enabled the cost-effective and rapid fabrication of devices for sample preparation, especially in flow-based mode, opening new possibilities for the development of automated analytical methods. Recent advances involve membrane-based three-dimensional printed separation devices fabricated by print-pause-print and multi-material three-dimensional printing, or improved three-dimensional printed holders for solid-phase extraction containing sorbent bead packings, extraction disks, fibers, and magnetic particles. Other recent developments rely on the direct three-dimensional printing of extraction sorbents, the functionalization of commercial three-dimensional printable resins, or the coating of three-dimensional printed devices with functional micro/nanomaterials. In addition, improved devices for liquid-liquid extraction such as extraction chambers, or phase separators are opening new possibilities for analytical method development combined with high-performance liquid chromatography. The present review outlines the current state-of-the-art of three-dimensional printing in analytical sample preparation. K E Y W O R D S3D printing, liquid-liquid extraction, membrane separation, sample preparation, solid-phase extraction 1854

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“…3D printing technology will be 3D modeling parts into 2D layered slices, according to the layered slice of the material stacked to the molding position, repeated stacking action caused by the workpiece molding, different from the traditional manufacturing model to cut the workpiece material subtraction way, often cause unnecessary cost consumption. 3D printing technology using layers of stacking method of production, it can avoid unnecessary waste of material [1].…”

Section: Laminated Manufacturing Technology (3d Printing)mentioning

confidence: 99%

“…In recent years, in the literature related research, Yuen [1] proposed a modelling method based on tectonics for the characteristics of blade design iteration, such as leaf design parameters, FDM production procedures, and to explore its use of PLA plastic material strength trends. Lee et al [2] fabricated helical vanes with laminated manufacturing techniques to explore their design parameters, such as leaf length, number of leaves, leaf turns, pitch height and other conditions, for the mixing efficiency of viscous liquids, to understand the feasibility of the laminated manufacturing technology spiral blades.…”

Section: Introductionmentioning

confidence: 99%

Simulation analysis of turbine blade in 3D printing aquarium

2017

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Abstract. 3D printing of the flexibility is the most admirable place, no matter when or where, as long as the machine can make the abstract design of finished products or difficult to process the finished product printed out as a sample. And in the product design, through the 3D print out the entity, to more specific observation of the advantages and disadvantages of finished products, which shorten the time of many creative research and development, but also relatively reduce the defective factors. As in recent years, 3D printing technology is progressing, material adhesion, precision and parts of the degree of cooperation has increased, coupled with many parts taking into account the cost, production and other issues, and then let a lot of light load small parts or special parts choose to use 3D to print the finished product to replace. This study focuses on the plastic turbine blades that drive water in the aquarium, but the 3D printing is done by stacking. However, the general stress analysis software can set the material to analyze the deformation results of the force, nor the 3D to analyze the software. Therefore, this study first analyzes the deformation of turbine blade by software, and then verifies the situation of 3D printing turbine blade, and then compares the actual results of software analysis and 3D printing. The results can provide the future of 3D product consider the strength factor. The study found that the spiral blade design allows the force points to be dispersed to avoid hard focus.

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Embedding objects during 3D printing to add new functionalities

Cited by 44 publications

References 29 publications

A 3D printed microfluidic perfusion device for multicellular spheroid cultures

A 3D printed microfluidic perfusion device for multicellular spheroid cultures

3D Printing in analytical sample preparation

Simulation analysis of turbine blade in 3D printing aquarium

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