Emamectin benzoate induces ROS-mediated DNA damage and apoptosis in Trichoplusia Tn5B1-4 cells

Luan, Shaorong; Yun, Xinming; Rao, Wenbing; Xiao, Ciying; Xu, Zhikang; Lang, Jialin; Huang, Qingchun

doi:10.1016/j.cbi.2017.06.004

Cited by 31 publications

(18 citation statements)

References 46 publications

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“…Emamectin benzoate (EB) is a macrocyclic lactone insecticide and acts by disrupting the nervous system, inhibiting muscle contraction, damaging the detoxic ability, and thereby leading to changes in metabolism and behavior of pest insects ( Isaac et al, 2002 ; Luan et al, 2017 ). With long residual ingestion activity on target arthropods and low toxicity to beneficial arthropods, EB is widely used for control of pest insects ( López et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Vitellogenin and Vitellogenin Receptor of Conopomorpha sinensis Bradley and Their Responses to Sublethal Concentrations of Insecticide

Yao

Dong

et al. 2018

Front. Physiol.

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Conopomorpha sinensis Bradley is the dominant borer pest of Litchi chinesis and Euphoria longan. Current management of C. sinensis relies upon insecticide application to adult moths. In addition to the direct mortality induced by insecticides, a sublethal dose of insecticides also affects growth, survival, and reproduction in the exposed insects. Vitellogenin (Vg) and vitellogenin receptor (VgR) are normally identified as essential reproduction-related proteins in insects. In this study, we characterized these two genes from C. sinensis, and investigated their differential responses to sublethal concentrations of insecticide. Cloned CsVg and CsVgR consist of 5391 and 5424-bp open reading frames, which encode proteins of 1796 and 1807 amino acid residues, respectively. The CsVg protein contains the typical vitellogenin, DUF1943 and VWFD domains as other reported lepidopteran Vgs. The CsVgR was characterized as a typical low density lipoprotein receptor with two highly conserved LBD and EGF precursor domains, one hydrophobic transmembrane domain, one cytoplasmic domain, and 13 putative N-glycosylation sites. We next assessed the sublethal effect of four major insecticides on egg-laying in C. sinensis. The toxicity against C. sinensis varied among the insecticides tested, with LC50 values ranging from 0.23 ppm for chlorpyrifos to 20.00 ppm for β-cypermethrin, among which emamectin benzoate (EB) showed a significant negative impact on egg-laying, survival rate, ovarian development, and mating rate of C. sinensis at LC30 doses. Further investigation showed that the transcriptional level of CsVg and CsVgR were impaired in different way at 24, 48, and 72 h after EB exposure, and this result was in agreement with the diminished egg-laying of C. sinensis in the sublethal concentration EB-treated group. A repressed transcription level of CsVgR was observed at 48 h after treatment, suggesting that EB elicits a delayed response in the abundance of CsVgR. These results established different roles of CsVg and CsVgR in response to the sublethal effect of insecticides. CsVg might be a better parameter than CsVgR for assessing the effect of sublethal insecticides on reproduction in C. sinensis.

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Section: Discussionmentioning

confidence: 99%

Characterization of Vitellogenin and Vitellogenin Receptor of Conopomorpha sinensis Bradley and Their Responses to Sublethal Concentrations of Insecticide

Yao

Dong

et al. 2018

Front. Physiol.

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“…Pesticides are significant threats to a honeybee’s life and can leading to impaired biochemical and physiological functions [47]. For instance, emamectin benzoate is a novel macrocyclic lactone insecticide that can induce intracellular ROS accumulation and cell apoptosis [48]. Paraquat is one of the most widely used herbicides in agriculture and is a strong redox agent that yields paraquat monocation radicals, which then react with molecular oxygen to produce superoxide anions [49].…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Wang

Cui

et al. 2018

Bioscience Reports

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Mitogen-activated protein kinase kinases (MKKs) are important components of the MAPK signaling pathways, which play a key role in responding to stress and inflammatory stimuli. Here, a new MKK gene, AccMKK6, was identified and functionally analyzed in Apis cerana cerana. Real-time quantitative PCR (qPCR) and Western blot analysis demonstrated that the AccMKK6 expression level was up-regulated by several environmental stresses. Moreover, the knockdown of AccMKK6 by RNA interference technology altered the expression levels of some antioxidant genes. In addition, the knockdown of AccMKK6 resulted in increased malonyldialdehyde (MDA) concentration and decreased antioxidant-related enzymes activity in honeybees. To explore the MAPK signaling pathways involved in AccMKK6, we identified the transcription factor kayak in A. cerana cerana. We analyzed the interactions of AccMKK6, Accp38b, and Acckayak using the yeast two-hybrid system. AccMKK6 and Acckayak showed similar expression profiles after several stress treatments. In addition, the expression level of Acckayak was significantly increased when AccMKK6 was silenced. Therefore, we speculate that AccMKK6 may be involved in the MAPK cascades, which play a crucial role in counteracting oxidative stress caused by external stimuli.

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“…Compared with that of avermectin, the insecticidal activity of emamectin benzoate is improved by 1-3 orders of magnitude, and it has very high activity against the larvae of lepidoptera insects, mites, and many other injurious insects. Emamectin benzoate has both the gastric toxicity and action of a contact poison, with a good effect at a very low dose (0.084~2 g/ha) [27]. After testing, the 0.1% emamectin benzoate used in the mite bait has slight toxicity through the skin, mouth, and nose.…”

Section: Discussionmentioning

confidence: 99%

Research on allergic rhinitis improvement in asthmatic children after dust mite exposure reduction: a randomized, double-blind, cross-placebo study protocol

Chen

Yuan

et al. 2020

Trials

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Background: Allergic rhinitis (AR) in children is a major respiratory inflammatory disease with a high incidence that is increasing yearly. In China, 54.93% of children with asthma have AR, which often requires synchronous treatment. House dust mites (HDMs) are common allergens that often cause attacks of AR and asthma. Reducing allergen exposure is one of the most important measures to control and treat AR and asthma attacks. Hestelia Mite Bait, containing 0.1% emamectin, is a new tool for trapping and killing dust mites, reducing the number of dust mites on mattresses and thereby potentially reducing stimulation by allergens and ultimately improving asthma and rhinitis symptoms. This single-centre, randomized, double-blind, cross-placebo trial will explore the improvement in AR in asthmatic children after dust mite exposure reduction. Methods: We will recruit 60 children (aged 3-12 years) who have been diagnosed with AR and asthma and are allergic to dust mites as confirmed by a serum allergen test. Participants will randomly receive the Hestelia Mite Bait intervention for 8 weeks and the placebo intervention for 8 weeks. There will be a 4-week washout period between the two interventions. The primary outcome is the visual analogue scale (VAS) score of AR symptoms; the secondary outcomes include the Rhinitis Control Assessment Test (RCAT) score, Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ) score, changes in the dust mite level, drug usage for asthma and AR, Asthma Control Questionnaire-5 (ACQ-5) score, and frequencies of acute asthma attacks, emergency visits, and hospitalizations. Discussion: This study aims to scientifically and objectively evaluate the effects of mite bait on rhinitis and asthma improvement after dust mite exposure reduction and provides a convenient means for future prevention and treatment of allergic diseases involving the airways in children.

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Emamectin benzoate induces ROS-mediated DNA damage and apoptosis in Trichoplusia Tn5B1-4 cells

Cited by 31 publications

References 46 publications

Characterization of Vitellogenin and Vitellogenin Receptor of Conopomorpha sinensis Bradley and Their Responses to Sublethal Concentrations of Insecticide

Characterization of Vitellogenin and Vitellogenin Receptor of Conopomorpha sinensis Bradley and Their Responses to Sublethal Concentrations of Insecticide

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Research on allergic rhinitis improvement in asthmatic children after dust mite exposure reduction: a randomized, double-blind, cross-placebo study protocol

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